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The map label for this gene is rlmH [H]

Identifier: 66047593

GI number: 66047593

Start: 5220479

End: 5220946

Strand: Reverse

Name: rlmH [H]

Synonym: Psyr_4366

Alternate gene names: 66047593

Gene position: 5220946-5220479 (Counterclockwise)

Preceding gene: 66047594

Following gene: 66047592

Centisome position: 85.68

GC content: 63.46

Gene sequence:

>468_bases
GTGCGTCTGCGTCTGATCGCTGTGGGTTCGCGCATGCCCAAGTGGGTGGAAGAAGGTTGGCACGAATATGCCAAGCGGAT
GCCCTCAGAGCTTGCGCTTGAACTGGTGGAAATCCCGCTCAATACCCGAGGCAAAAACGCCGACGTCGCGCGCTTTATCC
GTCAGGAAGGCGAGGCGATGCTGGCGAAAGTGCAGCCGGGCGAGCGGATTGTCACGCTGGAAGTGCAGGGCAAACCGTGG
AGCACCGAACAGTTGGCGGTCGAGCTCGATCGCTGGCGTCTCGATGCGCGCACGGTCAACCTGATGGTCGGCGGCCCCGA
GGGGCTGGCGCCTGAAGTCTGTGCCCGCAGTGAACAACGCTGGTCTCTGTCGCCGCTGACCCTGCCGCACCCGCTGGTGC
GCATCCTGATCGGCGAGCAGATGTATCGCGCCTGGACGGTCCTGTCCGGGCACCCTTATCACAAGTAG

Upstream 100 bases:

>100_bases
ACGCCAGTTCTATGACCTCGAGCGCTTGTGGCAGGGTGCTGAACAAAGCCGTGCTGCCAGCGCTGCGCACCACACACCGG
GCAACGAATAAGGTCTTGAT

Downstream 100 bases:

>100_bases
CATCGTCTTTACTGTGAATACGGCCTAAATGTCTCAACCGATCCGCCTCAAAGACCACGAAAAGGACGCACGCCTGGTGC
GCGGCAGGGTCGTGTTCGGT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MRLRLIAVGSRMPKWVEEGWHEYAKRMPSELALELVEIPLNTRGKNADVARFIRQEGEAMLAKVQPGERIVTLEVQGKPW
STEQLAVELDRWRLDARTVNLMVGGPEGLAPEVCARSEQRWSLSPLTLPHPLVRILIGEQMYRAWTVLSGHPYHK

Sequences:

>Translated_155_residues
MRLRLIAVGSRMPKWVEEGWHEYAKRMPSELALELVEIPLNTRGKNADVARFIRQEGEAMLAKVQPGERIVTLEVQGKPW
STEQLAVELDRWRLDARTVNLMVGGPEGLAPEVCARSEQRWSLSPLTLPHPLVRILIGEQMYRAWTVLSGHPYHK
>Mature_155_residues
MRLRLIAVGSRMPKWVEEGWHEYAKRMPSELALELVEIPLNTRGKNADVARFIRQEGEAMLAKVQPGERIVTLEVQGKPW
STEQLAVELDRWRLDARTVNLMVGGPEGLAPEVCARSEQRWSLSPLTLPHPLVRILIGEQMYRAWTVLSGHPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=56.7741935483871, Blast_Score=197, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17728; Mature: 17728

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRLIAVGSRMPKWVEEGWHEYAKRMPSELALELVEIPLNTRGKNADVARFIRQEGEAM
CEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEE
LAKVQPGERIVTLEVQGKPWSTEQLAVELDRWRLDARTVNLMVGGPEGLAPEVCARSEQR
EEEECCCCEEEEEEECCCCCCHHHHHHHHHHEEECCEEEEEEEECCCCCCHHHHCCCCCC
WSLSPLTLPHPLVRILIGEQMYRAWTVLSGHPYHK
CCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MRLRLIAVGSRMPKWVEEGWHEYAKRMPSELALELVEIPLNTRGKNADVARFIRQEGEAM
CEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEE
LAKVQPGERIVTLEVQGKPWSTEQLAVELDRWRLDARTVNLMVGGPEGLAPEVCARSEQR
EEEECCCCEEEEEEECCCCCCHHHHHHHHHHEEECCEEEEEEEECCCCCCHHHHCCCCCC
WSLSPLTLPHPLVRILIGEQMYRAWTVLSGHPYHK
CCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA