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The map label for this gene is lipB [H]

Identifier: 66047586

GI number: 66047586

Start: 5212943

End: 5213599

Strand: Reverse

Name: lipB [H]

Synonym: Psyr_4359

Alternate gene names: 66047586

Gene position: 5213599-5212943 (Counterclockwise)

Preceding gene: 66047587

Following gene: 66047585

Centisome position: 85.56

GC content: 60.27

Gene sequence:

>657_bases
ATGGCGGCTACGCTGGGTTTTCGTGATCTGGGCCTGATCGATTACGAAACCGCCTGGCACGCCATGAAGCGCTTTACCGA
CGAGCGTGGCCGCGAGGCTGCCGATGAGGTCTGGCTGGTTCAGCATCCGCCGGTTTTCACTCAGGGACAGTCCGGCAAGG
CCGAGCATTTGCTGCTGCCGGGTAATATTCCTGTCGTACAGGTCGATCGTGGCGGCCAAGTGACTTATCATGGTCCGGGC
CAACTGGTGGCTTATCTGATGCTGGACGTCAGGCGCCTTGGTTTCGGCGTTCGCGATCTGGTCACCCGGATCGAGAACAC
CCTCATCGCCTTGCTCGCCGACTACGGCGTCAAGGCAGCAGCCAAGGCTGATGCCCCTGGCGTATACGTCGACGGCGCGA
AGATAGCGTCGCTCGGTCTGCGCATTCGCAACGGCTGCTCGTTTCATGGTCTGGCGTTGAACGTCGACATGGACCTTGAG
CCGTTCCGCCGCATCAACCCGTGCGGATACGCCGGGCTGGCCATGACCCAACTGAGCGATCAGGCAGGGCAGATAGAATT
TTCCGAGGTAAGTGCCCGACTGCGTGCGCAGCTCGTCAAACACCTCGACTACGCTGAGCAGGCGACCCTTACGGGCGGAA
TAAACCATTATGACTGA

Upstream 100 bases:

>100_bases
ACACCACTGTGCAACTGCACATTGTCGCTACCGGCGAAGACCAGTTGCGCGATATCAATAGTGCCTTGCGCGCTACGGGT
TTCGTGCACATGGTGCTCTG

Downstream 100 bases:

>100_bases
CACCGTGCAAACCCTGATCCCGACGCTGGATGTTTCCGAGCGAGTGGCCCGTCCGAAAGTCGAAGCGGGCGTCAAGCTGC
GCGGAGCGGAAAAGGTTGCG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MAATLGFRDLGLIDYETAWHAMKRFTDERGREAADEVWLVQHPPVFTQGQSGKAEHLLLPGNIPVVQVDRGGQVTYHGPG
QLVAYLMLDVRRLGFGVRDLVTRIENTLIALLADYGVKAAAKADAPGVYVDGAKIASLGLRIRNGCSFHGLALNVDMDLE
PFRRINPCGYAGLAMTQLSDQAGQIEFSEVSARLRAQLVKHLDYAEQATLTGGINHYD

Sequences:

>Translated_218_residues
MAATLGFRDLGLIDYETAWHAMKRFTDERGREAADEVWLVQHPPVFTQGQSGKAEHLLLPGNIPVVQVDRGGQVTYHGPG
QLVAYLMLDVRRLGFGVRDLVTRIENTLIALLADYGVKAAAKADAPGVYVDGAKIASLGLRIRNGCSFHGLALNVDMDLE
PFRRINPCGYAGLAMTQLSDQAGQIEFSEVSARLRAQLVKHLDYAEQATLTGGINHYD
>Mature_217_residues
AATLGFRDLGLIDYETAWHAMKRFTDERGREAADEVWLVQHPPVFTQGQSGKAEHLLLPGNIPVVQVDRGGQVTYHGPGQ
LVAYLMLDVRRLGFGVRDLVTRIENTLIALLADYGVKAAAKADAPGVYVDGAKIASLGLRIRNGCSFHGLALNVDMDLEP
FRRINPCGYAGLAMTQLSDQAGQIEFSEVSARLRAQLVKHLDYAEQATLTGGINHYD

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=114, Percent_Identity=41.2280701754386, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI87081767, Length=205, Percent_Identity=55.609756097561, Blast_Score=241, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323268, Length=130, Percent_Identity=36.1538461538462, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355483, Length=148, Percent_Identity=32.4324324324324, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 23818; Mature: 23686

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATLGFRDLGLIDYETAWHAMKRFTDERGREAADEVWLVQHPPVFTQGQSGKAEHLLLP
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCEECCCCCCCCEEEEC
GNIPVVQVDRGGQVTYHGPGQLVAYLMLDVRRLGFGVRDLVTRIENTLIALLADYGVKAA
CCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
AKADAPGVYVDGAKIASLGLRIRNGCSFHGLALNVDMDLEPFRRINPCGYAGLAMTQLSD
CCCCCCCEEECCHHEEHHCEEECCCCCCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHCC
QAGQIEFSEVSARLRAQLVKHLDYAEQATLTGGINHYD
CCCCEEHHHHHHHHHHHHHHHHCHHHHHHEECCCCCCC
>Mature Secondary Structure 
AATLGFRDLGLIDYETAWHAMKRFTDERGREAADEVWLVQHPPVFTQGQSGKAEHLLLP
CCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCEECCCCCCCCEEEEC
GNIPVVQVDRGGQVTYHGPGQLVAYLMLDVRRLGFGVRDLVTRIENTLIALLADYGVKAA
CCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
AKADAPGVYVDGAKIASLGLRIRNGCSFHGLALNVDMDLEPFRRINPCGYAGLAMTQLSD
CCCCCCCEEECCHHEEHHCEEECCCCCCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHCC
QAGQIEFSEVSARLRAQLVKHLDYAEQATLTGGINHYD
CCCCEEHHHHHHHHHHHHHHHHCHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA