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The map label for this gene is lipA [H]

Identifier: 66047585

GI number: 66047585

Start: 5211943

End: 5212950

Strand: Reverse

Name: lipA [H]

Synonym: Psyr_4358

Alternate gene names: 66047585

Gene position: 5212950-5211943 (Counterclockwise)

Preceding gene: 66047586

Following gene: 66047578

Centisome position: 85.55

GC content: 62.1

Gene sequence:

>1008_bases
ATGACTGACACCGTGCAAACCCTGATCCCGACGCTGGATGTTTCCGAGCGAGTGGCCCGTCCGAAAGTCGAAGCGGGCGT
CAAGCTGCGCGGAGCGGAAAAGGTTGCGCGCATCCCGGTCAAGATCATCCCGACGGTAGATCTGCCGAAGAAACCCGACT
GGATTCGCGTGCGCATCCCGGTTTCTCCGGAAGTCGACCGCATCAAGCAATTGCTGCGCAAGCACAAGCTGCACAGCGTC
TGTGAAGAGGCGTCCTGCCCCAACCTGGGCGAATGCTTCTCCGGTGGCACTGCAACGTTCATGATCATGGGCGACATCTG
CACCCGTCGCTGCCCGTTCTGTGACGTGGGCCATGGTCGTCCGAAGCCGCTCGACGTCAACGAACCGAAGAGCCTCGCCA
TCGCGATTGCCGACCTGCGCCTGAAGTACGTGGTGATCACCTCGGTGGACCGCGACGACCTGCGTGACGGCGGTGCCCAG
CACTTCGCCGACTGCATCCGTGAAATCCGCCTGCTGTCGCCGGGCGTGCAGCTCGAAACGCTGGTGCCCGATTACCGTGG
CCGGATGGACGTGGCGCTGGAAATCACCGCCGCCGAGCCGCCGGATGTGTTCAACCACAACCTGGAAACCGTACCGCGTC
TGTACAAGGCTGCGCGTCCGGGGTCGGACTACCAGTGGTCGCTGACCCTGCTGCAGCGCTTCAAGCAGATGGTCCCGCAC
GTACCGACCAAGTCCGGGCTGATGCTGGGTTTGGGCGAGACCGACGAAGAAGTCATCGAAGTCATGCAGCGCATGCGCGA
ACACGACATCGACATGCTGACCCTGGGTCAATACCTGCAGCCTTCACGCAATCACTTGCCCGTGCAGCGCTTTGTCCACC
CGGACACCTTTGCCTGGTTCGCCGAAGAAGGCTACAAGATGGGCTTCAAGAACGTCGCTTCAGGGCCGCTGGTACGCTCT
TCGTACCACGCCGACGAGCAGGCCAAGATCGCCAAGGCGATGCTCTGA

Upstream 100 bases:

>100_bases
CAGATAGAATTTTCCGAGGTAAGTGCCCGACTGCGTGCGCAGCTCGTCAAACACCTCGACTACGCTGAGCAGGCGACCCT
TACGGGCGGAATAAACCATT

Downstream 100 bases:

>100_bases
CAGCACGCCGCAAAAACAGGAAGGGCCAGGTAGCGATACCTGGCCCTTCTTTTTTGCGGTGGATTATCGTGCCGATTCTC
TGCGGACTATCATGCCAACG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTDTVQTLIPTLDVSERVARPKVEAGVKLRGAEKVARIPVKIIPTVDLPKKPDWIRVRIPVSPEVDRIKQLLRKHKLHSV
CEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVNEPKSLAIAIADLRLKYVVITSVDRDDLRDGGAQ
HFADCIREIRLLSPGVQLETLVPDYRGRMDVALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMVPH
VPTKSGLMLGLGETDEEVIEVMQRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWFAEEGYKMGFKNVASGPLVRS
SYHADEQAKIAKAML

Sequences:

>Translated_335_residues
MTDTVQTLIPTLDVSERVARPKVEAGVKLRGAEKVARIPVKIIPTVDLPKKPDWIRVRIPVSPEVDRIKQLLRKHKLHSV
CEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVNEPKSLAIAIADLRLKYVVITSVDRDDLRDGGAQ
HFADCIREIRLLSPGVQLETLVPDYRGRMDVALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMVPH
VPTKSGLMLGLGETDEEVIEVMQRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWFAEEGYKMGFKNVASGPLVRS
SYHADEQAKIAKAML
>Mature_334_residues
TDTVQTLIPTLDVSERVARPKVEAGVKLRGAEKVARIPVKIIPTVDLPKKPDWIRVRIPVSPEVDRIKQLLRKHKLHSVC
EEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVNEPKSLAIAIADLRLKYVVITSVDRDDLRDGGAQH
FADCIREIRLLSPGVQLETLVPDYRGRMDVALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMVPHV
PTKSGLMLGLGETDEEVIEVMQRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWFAEEGYKMGFKNVASGPLVRSS
YHADEQAKIAKAML

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=47.8723404255319, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=48.1632653061224, Blast_Score=235, Evalue=5e-62,
Organism=Escherichia coli, GI1786846, Length=310, Percent_Identity=65.4838709677419, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32564533, Length=330, Percent_Identity=41.2121212121212, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=289, Percent_Identity=43.9446366782007, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=48.5815602836879, Blast_Score=268, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 37665; Mature: 37534

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTVQTLIPTLDVSERVARPKVEAGVKLRGAEKVARIPVKIIPTVDLPKKPDWIRVRIP
CCCCHHHHHCCCCHHHHHCCCHHHCCEEECCCHHHHHCCEEEEECCCCCCCCCEEEEEEC
VSPEVDRIKQLLRKHKLHSVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECHHHCCCCCCCCCCCC
PKPLDVNEPKSLAIAIADLRLKYVVITSVDRDDLRDGGAQHFADCIREIRLLSPGVQLET
CCCCCCCCCCCEEEEEEEEEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
LVPDYRGRMDVALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMVPH
ECCCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCC
VPTKSGLMLGLGETDEEVIEVMQRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWF
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHHCCCCHHHHH
AEEGYKMGFKNVASGPLVRSSYHADEQAKIAKAML
HHHCHHCCHHHHCCCCCEECCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TDTVQTLIPTLDVSERVARPKVEAGVKLRGAEKVARIPVKIIPTVDLPKKPDWIRVRIP
CCCHHHHHCCCCHHHHHCCCHHHCCEEECCCHHHHHCCEEEEECCCCCCCCCEEEEEEC
VSPEVDRIKQLLRKHKLHSVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECHHHCCCCCCCCCCCC
PKPLDVNEPKSLAIAIADLRLKYVVITSVDRDDLRDGGAQHFADCIREIRLLSPGVQLET
CCCCCCCCCCCEEEEEEEEEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
LVPDYRGRMDVALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMVPH
ECCCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCC
VPTKSGLMLGLGETDEEVIEVMQRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWF
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHHCCCCHHHHH
AEEGYKMGFKNVASGPLVRSSYHADEQAKIAKAML
HHHCHHCCHHHHCCCCCEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA