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The map label for this gene is hemL [H]

Identifier: 66047569

GI number: 66047569

Start: 5195661

End: 5196944

Strand: Direct

Name: hemL [H]

Synonym: Psyr_4342

Alternate gene names: 66047569

Gene position: 5195661-5196944 (Clockwise)

Preceding gene: 66047568

Following gene: 66047570

Centisome position: 85.26

GC content: 62.54

Gene sequence:

>1284_bases
ATGTCCCGTTCCGAAACACTGTTCGCCAACGCCCAAAAACACATCCCCGGTGGCGTCAATTCGCCGGTTCGCGCCTTCAA
GAGCGTGGGTGGCACGCCGCTGTTCTTCAAGCACGCTGCGGGCGCCTACGTGACTGACGAAGACGACAAGCGTTATGTGG
ATTACGTCGGCTCCTGGGGGCCGATGATTCTTGGCCATAGCCACCCCGATGTGCTCGATGCCGTGCGCAGCCAGCTGGAA
CATGGCCTGTCCTACGGTGCACCGACGGCCATGGAAACCGAAATGGCCGACCTGGTCTGCGAACTGGTGCCGTCCATGGA
AATGGTCCGCATGGTCAGCTCGGGCACTGAAGCGACCATGAGCGCGATCCGTCTGGCCCGTGGTTTCACCGGCCGCGACA
GCATCCTCAAGTTCGAAGGCTGCTACCACGGTCACTCCGACAGCCTGCTGGTCAAGGCCGGCTCCGGCGCACTGACCCTG
GGCGTGCCCAGCTCGCCTGGTGTACCCGCAGCTTTCGCGAAACACACTCTGACCGTGCCCTTCAACAACCTAGACGCCGT
GCGTGACCTGCTGGCCGAAGTGGGTCAGGAAGTGGCGTGCATCATCGTTGAACCCGTGGCCGGCAACATGAACTGTGTGC
CGCCGGCGCCTGGCTACCTGCAAGGGCTGCGCGAGCTGTGCGACGAGCACGGCGTGGTGCTGATTTTCGATGAAGTGATG
ACCGGTTTTCGCGTAGCCCTCGGCGGCGCACAGGCTTACTACGGCGTGACGCCCGACCTGAGCACCTTCGGCAAGATCAT
CGGTGGCGGCATGCCGGTCGGCTGCTTTGGCGGCAAACGCGAAATCATGTCGCACATCGCACCGCTCGGCCCGGTCTATC
AGGCCGGCACTCTGTCGGGCAACCCGCTGGCGATGGCGGCCGGCCTCACCACGCTGCGCCTGATCAGCCGTCCAGGCTTT
CACGATGAACTGAGCGACTACACCCGCCGCCTGCTCGAAGGTCTGCAACAGCGCGCCGATGCAGCCGGTATTCCGTTCGT
GACGACCCAGGCCGGCGGCATGTTCGGCCTGTATTTCAGCGAAGCCGACGAAATCGTCACGTTTGAAGACGTGATGACCA
GTGACTCCGAGCGCTTCAAGCGTTTCTTCCACCTGATGCTGGACGGCGGCGTGTACCTCGCGCCAAGCGCCTTCGAAGCG
GGTTTCACCTCAATCGCCCATGGTGATGCCGAGTTGAAACTGACCCTGGATGCCGCAGAAAAGGCATTCGCTGCGCTGAA
ATAA

Upstream 100 bases:

>100_bases
TGGCTCATGCCACCCAATAAAGCAGCCCACCCTGAAGCTGCCCGCCTCGGCTGACCCCAGCGTAGACACGAATTCCCCGC
TTCAAAGCAGAGACCCGATC

Downstream 100 bases:

>100_bases
GCTCCTCAGGCATCACTCGCGCAGGGTGTCAGTGAACCGGCACGAGTGATGACTGCAAATGAAACTGAACGTTAAGTATG
CATTTTGCTTCTACAATGCA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAAGAYVTDEDDKRYVDYVGSWGPMILGHSHPDVLDAVRSQLE
HGLSYGAPTAMETEMADLVCELVPSMEMVRMVSSGTEATMSAIRLARGFTGRDSILKFEGCYHGHSDSLLVKAGSGALTL
GVPSSPGVPAAFAKHTLTVPFNNLDAVRDLLAEVGQEVACIIVEPVAGNMNCVPPAPGYLQGLRELCDEHGVVLIFDEVM
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKREIMSHIAPLGPVYQAGTLSGNPLAMAAGLTTLRLISRPGF
HDELSDYTRRLLEGLQQRADAAGIPFVTTQAGGMFGLYFSEADEIVTFEDVMTSDSERFKRFFHLMLDGGVYLAPSAFEA
GFTSIAHGDAELKLTLDAAEKAFAALK

Sequences:

>Translated_427_residues
MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAAGAYVTDEDDKRYVDYVGSWGPMILGHSHPDVLDAVRSQLE
HGLSYGAPTAMETEMADLVCELVPSMEMVRMVSSGTEATMSAIRLARGFTGRDSILKFEGCYHGHSDSLLVKAGSGALTL
GVPSSPGVPAAFAKHTLTVPFNNLDAVRDLLAEVGQEVACIIVEPVAGNMNCVPPAPGYLQGLRELCDEHGVVLIFDEVM
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKREIMSHIAPLGPVYQAGTLSGNPLAMAAGLTTLRLISRPGF
HDELSDYTRRLLEGLQQRADAAGIPFVTTQAGGMFGLYFSEADEIVTFEDVMTSDSERFKRFFHLMLDGGVYLAPSAFEA
GFTSIAHGDAELKLTLDAAEKAFAALK
>Mature_426_residues
SRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAAGAYVTDEDDKRYVDYVGSWGPMILGHSHPDVLDAVRSQLEH
GLSYGAPTAMETEMADLVCELVPSMEMVRMVSSGTEATMSAIRLARGFTGRDSILKFEGCYHGHSDSLLVKAGSGALTLG
VPSSPGVPAAFAKHTLTVPFNNLDAVRDLLAEVGQEVACIIVEPVAGNMNCVPPAPGYLQGLRELCDEHGVVLIFDEVMT
GFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKREIMSHIAPLGPVYQAGTLSGNPLAMAAGLTTLRLISRPGFH
DELSDYTRRLLEGLQQRADAAGIPFVTTQAGGMFGLYFSEADEIVTFEDVMTSDSERFKRFFHLMLDGGVYLAPSAFEAG
FTSIAHGDAELKLTLDAAEKAFAALK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=325, Percent_Identity=28.9230769230769, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI13994255, Length=327, Percent_Identity=25.6880733944954, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI24119277, Length=327, Percent_Identity=26.605504587156, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI284507298, Length=237, Percent_Identity=30.379746835443, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=66.6666666666667, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1789016, Length=355, Percent_Identity=31.2676056338028, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789759, Length=306, Percent_Identity=31.0457516339869, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI145693181, Length=398, Percent_Identity=29.8994974874372, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788044, Length=309, Percent_Identity=31.7152103559871, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787560, Length=317, Percent_Identity=31.8611987381703, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1786991, Length=328, Percent_Identity=27.4390243902439, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=295, Percent_Identity=28.4745762711864, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=36.3636363636364, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17541228, Length=359, Percent_Identity=25.3481894150418, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=332, Percent_Identity=30.1204819277108, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=315, Percent_Identity=25.0793650793651, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357415, Length=348, Percent_Identity=25.8620689655172, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45394; Mature: 45263

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAAGAYVTDEDDKRYVDYVGSWG
CCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEECCCCCHHHHHHCCCC
PMILGHSHPDVLDAVRSQLEHGLSYGAPTAMETEMADLVCELVPSMEMVRMVSSGTEATM
CEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGFTGRDSILKFEGCYHGHSDSLLVKAGSGALTLGVPSSPGVPAAFAKHTLTVP
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHCCEEEEC
FNNLDAVRDLLAEVGQEVACIIVEPVAGNMNCVPPAPGYLQGLRELCDEHGVVLIFDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHH
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKREIMSHIAPLGPVYQAGTLSG
HHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCC
NPLAMAAGLTTLRLISRPGFHDELSDYTRRLLEGLQQRADAAGIPFVTTQAGGMFGLYFS
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEC
EADEIVTFEDVMTSDSERFKRFFHLMLDGGVYLAPSAFEAGFTSIAHGDAELKLTLDAAE
CCCCEEEHHHHHCCCHHHHHHHHHHHHCCCEEECCCHHHHCCHHHCCCCCEEEEEECHHH
KAFAALK
HHHHHCC
>Mature Secondary Structure 
SRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAAGAYVTDEDDKRYVDYVGSWG
CCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEECCCCCHHHHHHCCCC
PMILGHSHPDVLDAVRSQLEHGLSYGAPTAMETEMADLVCELVPSMEMVRMVSSGTEATM
CEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGFTGRDSILKFEGCYHGHSDSLLVKAGSGALTLGVPSSPGVPAAFAKHTLTVP
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHCCEEEEC
FNNLDAVRDLLAEVGQEVACIIVEPVAGNMNCVPPAPGYLQGLRELCDEHGVVLIFDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHH
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKREIMSHIAPLGPVYQAGTLSG
HHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCC
NPLAMAAGLTTLRLISRPGFHDELSDYTRRLLEGLQQRADAAGIPFVTTQAGGMFGLYFS
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEC
EADEIVTFEDVMTSDSERFKRFFHLMLDGGVYLAPSAFEAGFTSIAHGDAELKLTLDAAE
CCCCEEEHHHHHCCCHHHHHHHHHHHHCCCEEECCCHHHHCCHHHCCCCCEEEEEECHHH
KAFAALK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA