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The map label for this gene is thiE [H]

Identifier: 66047568

GI number: 66047568

Start: 5194772

End: 5195389

Strand: Direct

Name: thiE [H]

Synonym: Psyr_4341

Alternate gene names: 66047568

Gene position: 5194772-5195389 (Clockwise)

Preceding gene: 66047567

Following gene: 66047569

Centisome position: 85.25

GC content: 63.59

Gene sequence:

>618_bases
ATGAAACTGCGTGGCCTGTATGCCATCACCGACAGCCAGTTGCTGTCCGGAAAATTTCTGTCGTACGTCGAAGCGGCGCT
GGACGGCGGCGTGACGCTGCTGCAATACCGCGACAAGAACAGTGACGAGTCCCGCCGCCTGCGCGAAGCGACCGAACTGC
TCAAGCTCTGCGAACGCTACAAGACCCGGCTGATCATCAACGATGACGCTGAACTGGCTGCGCGGCTGGGGGTGGGCGTG
CATTTGGGACAGTCCGACGGCTCGCTGCCCGATGCCCGCGCCCTGCTCGGGCACAAGGCGATTGTCGGGGCTACTTGTCA
TGGCCGCGTGGAATTGGCCGAGCAAGCCAAGGCTGACGGCGCGACGTACGTGGCATTTGGCAGGTTCTTCAACTCCCTGA
CCAAACCCGGCGCCCCGGCCGTGCCGCTGGACCTGATCGCTCAGGTGCGTGCCAGGGTCCACCTGCCGATTGCGGTGATT
GGCGGCATTACCCTGGAGAACGCACCGCAACTGGTCGAACACGGGGCCGACCTGCTCGCCGTGGTGCACGGCCTGTTCGG
CGCTGAAAATACTCAGGAAGTGACGCGCCGGGCGAAAGCTTTCACGGCGTTGTTGTAG

Upstream 100 bases:

>100_bases
GAACGCTGAGGGATGCCGAACAGTTGGGCCGGGGGCAATTCGTGCCACGTCGGCTGCCGCTGGATTTTTGCTCGTAACAC
ATCGCCATGAGGGTCGATTC

Downstream 100 bases:

>100_bases
AGCGGAATAGTTTTGGGCGCGAGCCGGGCGACGCTTCGCTTGCTCGCGAAGGCGATGGTTTAGACCCGCTATTTTCGGGG
CCTGCATTGCCCCCTTCGCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKLRGLYAITDSQLLSGKFLSYVEAALDGGVTLLQYRDKNSDESRRLREATELLKLCERYKTRLIINDDAELAARLGVGV
HLGQSDGSLPDARALLGHKAIVGATCHGRVELAEQAKADGATYVAFGRFFNSLTKPGAPAVPLDLIAQVRARVHLPIAVI
GGITLENAPQLVEHGADLLAVVHGLFGAENTQEVTRRAKAFTALL

Sequences:

>Translated_205_residues
MKLRGLYAITDSQLLSGKFLSYVEAALDGGVTLLQYRDKNSDESRRLREATELLKLCERYKTRLIINDDAELAARLGVGV
HLGQSDGSLPDARALLGHKAIVGATCHGRVELAEQAKADGATYVAFGRFFNSLTKPGAPAVPLDLIAQVRARVHLPIAVI
GGITLENAPQLVEHGADLLAVVHGLFGAENTQEVTRRAKAFTALL
>Mature_205_residues
MKLRGLYAITDSQLLSGKFLSYVEAALDGGVTLLQYRDKNSDESRRLREATELLKLCERYKTRLIINDDAELAARLGVGV
HLGQSDGSLPDARALLGHKAIVGATCHGRVELAEQAKADGATYVAFGRFFNSLTKPGAPAVPLDLIAQVRARVHLPIAVI
GGITLENAPQLVEHGADLLAVVHGLFGAENTQEVTRRAKAFTALL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=194, Percent_Identity=32.4742268041237, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=143, Percent_Identity=37.0629370629371, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22023; Mature: 22023

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRGLYAITDSQLLSGKFLSYVEAALDGGVTLLQYRDKNSDESRRLREATELLKLCERY
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
KTRLIINDDAELAARLGVGVHLGQSDGSLPDARALLGHKAIVGATCHGRVELAEQAKADG
HCEEEECCCHHHHHHHCCCEEECCCCCCCCHHHHHHHCHHHEECCCCCHHHHHHHHCCCC
ATYVAFGRFFNSLTKPGAPAVPLDLIAQVRARVHLPIAVIGGITLENAPQLVEHGADLLA
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHCCHHHHH
VVHGLFGAENTQEVTRRAKAFTALL
HHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLRGLYAITDSQLLSGKFLSYVEAALDGGVTLLQYRDKNSDESRRLREATELLKLCERY
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
KTRLIINDDAELAARLGVGVHLGQSDGSLPDARALLGHKAIVGATCHGRVELAEQAKADG
HCEEEECCCHHHHHHHCCCEEECCCCCCCCHHHHHHHCHHHEECCCCCHHHHHHHHCCCC
ATYVAFGRFFNSLTKPGAPAVPLDLIAQVRARVHLPIAVIGGITLENAPQLVEHGADLLA
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHCCHHHHH
VVHGLFGAENTQEVTRRAKAFTALL
HHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA