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The map label for this gene is trmA [H]

Identifier: 66047514

GI number: 66047514

Start: 5112792

End: 5113877

Strand: Direct

Name: trmA [H]

Synonym: Psyr_4287

Alternate gene names: 66047514

Gene position: 5112792-5113877 (Clockwise)

Preceding gene: 66047513

Following gene: 66047515

Centisome position: 83.9

GC content: 63.9

Gene sequence:

>1086_bases
ATGAGTGTTCCCTTCGACCCTGCGAGCTACGACCGTCAGCTCGAAGAAAAAACCGTCCGCCTGCGCGAGTTGCTGGCGCC
GTTCGATGCTCCCGAGCCACAGGTGTTCGACTCGCCACGCGAACACTATAGGCTGCGCGCCGAGTTCCGCCTGTGGCGCG
AAGACCAGAAGCGCTATTACGCGATGTTCGCGCCGGGCGACAACAGGACGCCGATCCTGCTCGAGGGCCTGCCGATCGCC
AGCGAGCGTATCAACGCGCTGATGCCAGTGCTGCGCGAGCGCTGGGAAGCCAGCCCGACGTTGAACCACAAGCTGTTTCA
GGTGGACTTCCTGACCACGCTGGCAGGCGACGCGATGATCACCCTGTGTTATCACCGCCCGCTGGACGCCGAATGGCAGG
CCGCCGCCGCGCAACTGGCCGCCGAACTGGACGTCAGCCTGATTGGCCGCTCCAAGGGGCAGAAGCTGGTCATCGGTCAT
GATTACGTGACCGAGAAGCTCGACGTGGCTGGCCGCACCTTCAGTTACCGGCAACCGGAAGGCGCTTTCACCCAGCCCAA
CGGCACGGTGAACGGCAAGATGCTCAACTGGGCCTTCGATGCGCTGGGCGAGCGGCAGGACGACCTGCTGGAGCTGTATT
GCGGCAATGGCAACTTCACCCTGCCGCTGGCTACACGCGTGCGCAAGGTGCTGGCGACCGAAATCAGCAAGACCTCGGTC
AATGCGGCGCTGAGCAACCTGGATGACAACGGCGTGGACAACGTCACGCTGGTGCGTCTGTCGGCCGAAGAACTGACCGA
AGCCCTGAACGAAGTGCGCCCGTTCCGCCGCCTGCACGGCGTGGACTTGAAGAGCTACGATTTCGGCAGCGTGTTCGTCG
ACCCGCCGCGCGCCGGCATGGACCCGGACACCTGCGAACTGACCCGGCGCTTCGAGCGCATTCTGTACATATCCTGCAAC
CCGGAAACCCTGGCCGCCAACATCGCCCAACTGCACGACACCCACCGGGTCGAGCGCTGCGCGCTGTTCGATCAGTTTCC
GTACACCCACCACATGGAGTCAGGCGTGCTGCTGGTTAGGCGCTGA

Upstream 100 bases:

>100_bases
GGACAGCGATCAAGCTGCTGTCGGGACGCTGGCGCGAGCTGAATTCGGCGCTGGTGATCCTGTCGATCCTGTTCGTGATC
AAGCTGGGCTGGTTCAACGC

Downstream 100 bases:

>100_bases
GGCTCCTCTGACTGACTATCGTGCCGACGCTCCGCGTTGGCATGCCGTTCTGGACGCTGCGCGTCCTATCCTGAACATGC
GGCGCGACGCAGATCTGTGA

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSVPFDPASYDRQLEEKTVRLRELLAPFDAPEPQVFDSPREHYRLRAEFRLWREDQKRYYAMFAPGDNRTPILLEGLPIA
SERINALMPVLRERWEASPTLNHKLFQVDFLTTLAGDAMITLCYHRPLDAEWQAAAAQLAAELDVSLIGRSKGQKLVIGH
DYVTEKLDVAGRTFSYRQPEGAFTQPNGTVNGKMLNWAFDALGERQDDLLELYCGNGNFTLPLATRVRKVLATEISKTSV
NAALSNLDDNGVDNVTLVRLSAEELTEALNEVRPFRRLHGVDLKSYDFGSVFVDPPRAGMDPDTCELTRRFERILYISCN
PETLAANIAQLHDTHRVERCALFDQFPYTHHMESGVLLVRR

Sequences:

>Translated_361_residues
MSVPFDPASYDRQLEEKTVRLRELLAPFDAPEPQVFDSPREHYRLRAEFRLWREDQKRYYAMFAPGDNRTPILLEGLPIA
SERINALMPVLRERWEASPTLNHKLFQVDFLTTLAGDAMITLCYHRPLDAEWQAAAAQLAAELDVSLIGRSKGQKLVIGH
DYVTEKLDVAGRTFSYRQPEGAFTQPNGTVNGKMLNWAFDALGERQDDLLELYCGNGNFTLPLATRVRKVLATEISKTSV
NAALSNLDDNGVDNVTLVRLSAEELTEALNEVRPFRRLHGVDLKSYDFGSVFVDPPRAGMDPDTCELTRRFERILYISCN
PETLAANIAQLHDTHRVERCALFDQFPYTHHMESGVLLVRR
>Mature_360_residues
SVPFDPASYDRQLEEKTVRLRELLAPFDAPEPQVFDSPREHYRLRAEFRLWREDQKRYYAMFAPGDNRTPILLEGLPIAS
ERINALMPVLRERWEASPTLNHKLFQVDFLTTLAGDAMITLCYHRPLDAEWQAAAAQLAAELDVSLIGRSKGQKLVIGHD
YVTEKLDVAGRTFSYRQPEGAFTQPNGTVNGKMLNWAFDALGERQDDLLELYCGNGNFTLPLATRVRKVLATEISKTSVN
AALSNLDDNGVDNVTLVRLSAEELTEALNEVRPFRRLHGVDLKSYDFGSVFVDPPRAGMDPDTCELTRRFERILYISCNP
ETLAANIAQLHDTHRVERCALFDQFPYTHHMESGVLLVRR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=357, Percent_Identity=50.9803921568627, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789148, Length=362, Percent_Identity=30.939226519337, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787083, Length=211, Percent_Identity=29.3838862559242, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24666579, Length=229, Percent_Identity=25.764192139738, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 41053; Mature: 40922

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPFDPASYDRQLEEKTVRLRELLAPFDAPEPQVFDSPREHYRLRAEFRLWREDQKRYY
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEHHHHHHHHHCCCEEE
AMFAPGDNRTPILLEGLPIASERINALMPVLRERWEASPTLNHKLFQVDFLTTLAGDAMI
EEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCEEE
TLCYHRPLDAEWQAAAAQLAAELDVSLIGRSKGQKLVIGHDYVTEKLDVAGRTFSYRQPE
EEEECCCCCCHHHHHHHHHHHHCCHHEEECCCCCEEEEECHHHHHHHHHCCCCCCCCCCC
GAFTQPNGTVNGKMLNWAFDALGERQDDLLELYCGNGNFTLPLATRVRKVLATEISKTSV
CCCCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHH
NAALSNLDDNGVDNVTLVRLSAEELTEALNEVRPFRRLHGVDLKSYDFGSVFVDPPRAGM
HHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
DPDTCELTRRFERILYISCNPETLAANIAQLHDTHRVERCALFDQFPYTHHMESGVLLVR
CCHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
R
C
>Mature Secondary Structure 
SVPFDPASYDRQLEEKTVRLRELLAPFDAPEPQVFDSPREHYRLRAEFRLWREDQKRYY
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEHHHHHHHHHCCCEEE
AMFAPGDNRTPILLEGLPIASERINALMPVLRERWEASPTLNHKLFQVDFLTTLAGDAMI
EEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCEEE
TLCYHRPLDAEWQAAAAQLAAELDVSLIGRSKGQKLVIGHDYVTEKLDVAGRTFSYRQPE
EEEECCCCCCHHHHHHHHHHHHCCHHEEECCCCCEEEEECHHHHHHHHHCCCCCCCCCCC
GAFTQPNGTVNGKMLNWAFDALGERQDDLLELYCGNGNFTLPLATRVRKVLATEISKTSV
CCCCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHH
NAALSNLDDNGVDNVTLVRLSAEELTEALNEVRPFRRLHGVDLKSYDFGSVFVDPPRAGM
HHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
DPDTCELTRRFERILYISCNPETLAANIAQLHDTHRVERCALFDQFPYTHHMESGVLLVR
CCHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA