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The map label for this gene is nylA [H]

Identifier: 66047495

GI number: 66047495

Start: 5089512

End: 5090963

Strand: Direct

Name: nylA [H]

Synonym: Psyr_4268

Alternate gene names: 66047495

Gene position: 5089512-5090963 (Clockwise)

Preceding gene: 66047494

Following gene: 66047497

Centisome position: 83.52

GC content: 65.98

Gene sequence:

>1452_bases
ATGAACGACCTGCACGAATTATTCAATACGCTGGACGGCCGTGGCCTGGCAACACTGGTACGCAATGGCGAGGTGAGACC
CGGCGAGCTGCTGGAAACCGCTATCGCGCGGGTCGAGCAGGTCGAGCCCGGCCTGAACGCCGTCAGCGAAAGGCTTTACG
AGCAGGCGCGCAGCCAGGCGAGCGGAAGCAGCGTCAGACAGGGCGTCATGGCAGGCGTACCGACCCTGGTGAAAGACATG
TTCACCCCGATGACCGGAGCACGGATGACCAGTGGCTCGCATGCGCTGGGCGAGTTTCGCTCAAACATCGACTGCGAAAT
CGTCAGCCGCCTGCGCCAGGCCGGCTGCCAACTGATCGGAACGACCACCGCCCCAGAATTCGGCGTGTCCTACAGCACCG
AATCGCAACGCTTCGGCGCGACCTGCAATCCATGGAATCTGCAGCACAGCGCGGGTGGTTCCAGCGGGGGTGCCGCCGCT
CTGGTGGCGGCGCGCGCCATCCCCTTCGCTCATGGCAACGACGGCGGCGGCTCGCTTCGCGTACCGGCCTCCTGCTGCGG
CGTATTCGGCTTCAAGCCCTCGCGCGGCCTGCTGCCGTCCGGCCCGATGGTCGGCGAGGGCTGGGCAGGCCTGAGCACCG
CTCATGCGATTACCCTGTCGGTAGGCGACAGCGCCGCCCTGCTTGATGCCACTGCCGGGAGTGACCTGGGGGCTCCCTAT
GCGGCGCCCATGCCAGCGACGCCCTTTGCACACGCCGCAGGACGCGATCCCAGGCGCCTGAAAATCGCCCTGATCTCGGC
AATATCACCCTGGCCGGCCGAACCGGAAGCCCTGGCCGCACTGCAACACACTGCGGCCTTGCTGGAGGCGCTCGGGCATT
ACGTGGTCCCGACGACGTTGCCGGTTCAGTTACCTGAATTCCTCGATGCGACGTTCGACATCATCGGCTCACACACCCAG
GGCTACCTCGATCTGCTGGGCAAAATGCGCGGTTTCCCGGTGGCCATGGATGAACTGGAGCCGCGCACCCGTGTGATTCT
GCGCGAACGCGGGCAACTGCCTGCAACACGCTACATTTCCGCGGTCGAATCGATGCATGCACTGGGCCGGGCGATGGCCG
GTTTCTTCGAAGAGTACGACATTGTTCTCACCCCGACGCTCAACCGCGCGCCGCCCCGCCTGGGGGAGCTGGCCTTCGAC
GACGACAGCCGCTCCCTGCAGGACCTCATCGCGCTTTCGCACCGCTACTCGCCCTACACGGCGATCTTCAACGCGACAGG
CCAGCCGGCCATGTCCGTGCCCCTCTACTGGACAGCCGATTCGCTGCCGCTGGGCTCTCACTTCGCCGCGCGGTTTGGCG
ACGAACTGACCTTGCTGAGCCTCGCCGGGCAACTGGAACGCGCCCAGCCGTGGGCCGCACGCAGGCCGCCGCTAAACGCT
TGTGCGGGTTGA

Upstream 100 bases:

>100_bases
GCAACCGTGCCGAGGGCTACACCGTGGCGCTGCGTACCGGCATCAGCTTTTAATTGGCGTGCGCACCGCGACGACACGCT
TTCAATCCTGGAGACGATCG

Downstream 100 bases:

>100_bases
TGCGAAGGGATTATTGGCGGTGGCGAGACCACCGCCTGCGTTGCAGGCGGCGGTCTGCCGGCTACAGGCTTATGGCTCGC
CAGCCGCTTGCTGTGCCGGC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNDLHELFNTLDGRGLATLVRNGEVRPGELLETAIARVEQVEPGLNAVSERLYEQARSQASGSSVRQGVMAGVPTLVKDM
FTPMTGARMTSGSHALGEFRSNIDCEIVSRLRQAGCQLIGTTTAPEFGVSYSTESQRFGATCNPWNLQHSAGGSSGGAAA
LVAARAIPFAHGNDGGGSLRVPASCCGVFGFKPSRGLLPSGPMVGEGWAGLSTAHAITLSVGDSAALLDATAGSDLGAPY
AAPMPATPFAHAAGRDPRRLKIALISAISPWPAEPEALAALQHTAALLEALGHYVVPTTLPVQLPEFLDATFDIIGSHTQ
GYLDLLGKMRGFPVAMDELEPRTRVILRERGQLPATRYISAVESMHALGRAMAGFFEEYDIVLTPTLNRAPPRLGELAFD
DDSRSLQDLIALSHRYSPYTAIFNATGQPAMSVPLYWTADSLPLGSHFAARFGDELTLLSLAGQLERAQPWAARRPPLNA
CAG

Sequences:

>Translated_483_residues
MNDLHELFNTLDGRGLATLVRNGEVRPGELLETAIARVEQVEPGLNAVSERLYEQARSQASGSSVRQGVMAGVPTLVKDM
FTPMTGARMTSGSHALGEFRSNIDCEIVSRLRQAGCQLIGTTTAPEFGVSYSTESQRFGATCNPWNLQHSAGGSSGGAAA
LVAARAIPFAHGNDGGGSLRVPASCCGVFGFKPSRGLLPSGPMVGEGWAGLSTAHAITLSVGDSAALLDATAGSDLGAPY
AAPMPATPFAHAAGRDPRRLKIALISAISPWPAEPEALAALQHTAALLEALGHYVVPTTLPVQLPEFLDATFDIIGSHTQ
GYLDLLGKMRGFPVAMDELEPRTRVILRERGQLPATRYISAVESMHALGRAMAGFFEEYDIVLTPTLNRAPPRLGELAFD
DDSRSLQDLIALSHRYSPYTAIFNATGQPAMSVPLYWTADSLPLGSHFAARFGDELTLLSLAGQLERAQPWAARRPPLNA
CAG
>Mature_483_residues
MNDLHELFNTLDGRGLATLVRNGEVRPGELLETAIARVEQVEPGLNAVSERLYEQARSQASGSSVRQGVMAGVPTLVKDM
FTPMTGARMTSGSHALGEFRSNIDCEIVSRLRQAGCQLIGTTTAPEFGVSYSTESQRFGATCNPWNLQHSAGGSSGGAAA
LVAARAIPFAHGNDGGGSLRVPASCCGVFGFKPSRGLLPSGPMVGEGWAGLSTAHAITLSVGDSAALLDATAGSDLGAPY
AAPMPATPFAHAAGRDPRRLKIALISAISPWPAEPEALAALQHTAALLEALGHYVVPTTLPVQLPEFLDATFDIIGSHTQ
GYLDLLGKMRGFPVAMDELEPRTRVILRERGQLPATRYISAVESMHALGRAMAGFFEEYDIVLTPTLNRAPPRLGELAFD
DDSRSLQDLIALSHRYSPYTAIFNATGQPAMSVPLYWTADSLPLGSHFAARFGDELTLLSLAGQLERAQPWAARRPPLNA
CAG

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=25.2918287937743, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI195972892, Length=203, Percent_Identity=31.0344827586207, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI166795287, Length=316, Percent_Identity=29.746835443038, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=308, Percent_Identity=28.2467532467532, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=195, Percent_Identity=30.7692307692308, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=342, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=161, Percent_Identity=33.5403726708075, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=319, Percent_Identity=23.5109717868339, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=307, Percent_Identity=27.0358306188925, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=27.0358306188925, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=218, Percent_Identity=29.3577981651376, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356731, Length=193, Percent_Identity=35.7512953367876, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=197, Percent_Identity=36.5482233502538, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550774, Length=192, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648435, Length=192, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=192, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161078093, Length=528, Percent_Identity=24.6212121212121, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652985, Length=193, Percent_Identity=30.5699481865285, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922090, Length=193, Percent_Identity=30.5699481865285, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652981, Length=193, Percent_Identity=30.5699481865285, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652983, Length=193, Percent_Identity=30.5699481865285, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=26.5182186234818, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51123; Mature: 51123

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLHELFNTLDGRGLATLVRNGEVRPGELLETAIARVEQVEPGLNAVSERLYEQARSQA
CCHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
SGSSVRQGVMAGVPTLVKDMFTPMTGARMTSGSHALGEFRSNIDCEIVSRLRQAGCQLIG
CCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEEE
TTTAPEFGVSYSTESQRFGATCNPWNLQHSAGGSSGGAAALVAARAIPFAHGNDGGGSLR
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
VPASCCGVFGFKPSRGLLPSGPMVGEGWAGLSTAHAITLSVGDSAALLDATAGSDLGAPY
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCC
AAPMPATPFAHAAGRDPRRLKIALISAISPWPAEPEALAALQHTAALLEALGHYVVPTTL
CCCCCCCCCCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEECCCCC
PVQLPEFLDATFDIIGSHTQGYLDLLGKMRGFPVAMDELEPRTRVILRERGQLPATRYIS
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHCCHHHHHHHHHCCCCCHHHHHH
AVESMHALGRAMAGFFEEYDIVLTPTLNRAPPRLGELAFDDDSRSLQDLIALSHRYSPYT
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEE
AIFNATGQPAMSVPLYWTADSLPLGSHFAARFGDELTLLSLAGQLERAQPWAARRPPLNA
EEEECCCCCCEECEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHH
CAG
CCC
>Mature Secondary Structure
MNDLHELFNTLDGRGLATLVRNGEVRPGELLETAIARVEQVEPGLNAVSERLYEQARSQA
CCHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
SGSSVRQGVMAGVPTLVKDMFTPMTGARMTSGSHALGEFRSNIDCEIVSRLRQAGCQLIG
CCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEEE
TTTAPEFGVSYSTESQRFGATCNPWNLQHSAGGSSGGAAALVAARAIPFAHGNDGGGSLR
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
VPASCCGVFGFKPSRGLLPSGPMVGEGWAGLSTAHAITLSVGDSAALLDATAGSDLGAPY
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCC
AAPMPATPFAHAAGRDPRRLKIALISAISPWPAEPEALAALQHTAALLEALGHYVVPTTL
CCCCCCCCCCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEECCCCC
PVQLPEFLDATFDIIGSHTQGYLDLLGKMRGFPVAMDELEPRTRVILRERGQLPATRYIS
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHCCHHHHHHHHHCCCCCHHHHHH
AVESMHALGRAMAGFFEEYDIVLTPTLNRAPPRLGELAFDDDSRSLQDLIALSHRYSPYT
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEE
AIFNATGQPAMSVPLYWTADSLPLGSHFAARFGDELTLLSLAGQLERAQPWAARRPPLNA
EEEECCCCCCEECEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHH
CAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]