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The map label for this gene is gsiC [H]

Identifier: 66047442

GI number: 66047442

Start: 5020381

End: 5021328

Strand: Reverse

Name: gsiC [H]

Synonym: Psyr_4215

Alternate gene names: 66047442

Gene position: 5021328-5020381 (Counterclockwise)

Preceding gene: 66047447

Following gene: 66047441

Centisome position: 82.4

GC content: 61.08

Gene sequence:

>948_bases
ATGAATCTGGGTCGTTATCGTTTTATTCTCCAGCGTCCGCTGCAGCTGCTGCCGGTGCTGTTCGGCATCAGCCTGGTCAC
CTTCATTCTGGTGCGTTCGATCCCCGGCGATCCGGCGCGCATGCTGCTGGGTTCGCGTTCCACACCACAGGCCCTGGCCA
ACATCCGCGCCCAGTATGGTCTGGATGAGCCTCTGTGGACACAGTACGTGTATTTCATGAAAAACCTGGTGCAGGGCGAT
ATGGGCAAATCGCTGCTGTACAAGATAGACGTACTCAAGCTGATCAGCACACGGATCGAGCCCACCGTATTTCTGGTGTC
GGGCAGCGTTCTGCTGGCGCTGCTGATTGCCGTGCCCCTGGCCACGCTCGCTGCGCGTAACAAAGGCAGTTGGAGCGACA
ACCTGATCCGCGTCTTTACCACCACCGGGCTGGGCATGCCGGCGTTCTGGCTGGGCATCATGCTGATCCTGCTGTTCAGT
GTGCAGCTTGGCTGGTTTCCGGTGTCGGGCTACGGCAGCGACTGGCTGGACAAGGCCCATCACCTGGTGCTGCCCTGTCT
GACCATCGCGCTGGCTCTGTCCGCGGTGCTGATTCGCAACCTGCGCGCGAGCATGCTCGTGGAGCTGCAAGCCGATCATG
TGACGGCGGCCCGGGCGCGTGGCCTCAGTGAAGGTGCGCTGTTTCGGCGCCACGTGTTGCCCAATTCGCTGGTGCCTTCG
ATCAACCTGCTGGCGGTGAACATCGGCTGGCTGATCAGCGGCACCGTGGTGATCGAGAGTCTGTTTGCGATTCCCGGCAT
CGGTCAGTTGCTGGTGCGCGGCATCTTCAGTCGCGATTACATGGTGGTGCAGGGGGTGGCCATGGTGCTGGCCTGTGCCA
CGGTGATCGTGAACTTCCTGGCCGATGTGGCCACGGTGGCCATTGACCCGCGGGTGAATATCAAATGA

Upstream 100 bases:

>100_bases
CAATGTTCGACTACAACGTTGCTCTGCCCTTTTACGTAGAGGGGGCGGCGGCGTACTTTCCGGTCCTGCTGAATTTTCTG
TCACAGCATCGGGGCGTCTG

Downstream 100 bases:

>100_bases
CCGTGGCCCATGCCGATCTTCGTCCGCACTGGCGTTCCCGGCTGCGCCTGCGTTTGCGCAATGGCCGGCTGGCGATGCTG
CTGGGCGGCTCTTTATTGGG

Product: binding-protein dependent transport system inner membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNLGRYRFILQRPLQLLPVLFGISLVTFILVRSIPGDPARMLLGSRSTPQALANIRAQYGLDEPLWTQYVYFMKNLVQGD
MGKSLLYKIDVLKLISTRIEPTVFLVSGSVLLALLIAVPLATLAARNKGSWSDNLIRVFTTTGLGMPAFWLGIMLILLFS
VQLGWFPVSGYGSDWLDKAHHLVLPCLTIALALSAVLIRNLRASMLVELQADHVTAARARGLSEGALFRRHVLPNSLVPS
INLLAVNIGWLISGTVVIESLFAIPGIGQLLVRGIFSRDYMVVQGVAMVLACATVIVNFLADVATVAIDPRVNIK

Sequences:

>Translated_315_residues
MNLGRYRFILQRPLQLLPVLFGISLVTFILVRSIPGDPARMLLGSRSTPQALANIRAQYGLDEPLWTQYVYFMKNLVQGD
MGKSLLYKIDVLKLISTRIEPTVFLVSGSVLLALLIAVPLATLAARNKGSWSDNLIRVFTTTGLGMPAFWLGIMLILLFS
VQLGWFPVSGYGSDWLDKAHHLVLPCLTIALALSAVLIRNLRASMLVELQADHVTAARARGLSEGALFRRHVLPNSLVPS
INLLAVNIGWLISGTVVIESLFAIPGIGQLLVRGIFSRDYMVVQGVAMVLACATVIVNFLADVATVAIDPRVNIK
>Mature_315_residues
MNLGRYRFILQRPLQLLPVLFGISLVTFILVRSIPGDPARMLLGSRSTPQALANIRAQYGLDEPLWTQYVYFMKNLVQGD
MGKSLLYKIDVLKLISTRIEPTVFLVSGSVLLALLIAVPLATLAARNKGSWSDNLIRVFTTTGLGMPAFWLGIMLILLFS
VQLGWFPVSGYGSDWLDKAHHLVLPCLTIALALSAVLIRNLRASMLVELQADHVTAARARGLSEGALFRRHVLPNSLVPS
INLLAVNIGWLISGTVVIESLFAIPGIGQLLVRGIFSRDYMVVQGVAMVLACATVIVNFLADVATVAIDPRVNIK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=35.7142857142857, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1789888, Length=310, Percent_Identity=37.741935483871, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789965, Length=336, Percent_Identity=32.7380952380952, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787761, Length=327, Percent_Identity=37.3088685015291, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787497, Length=308, Percent_Identity=31.8181818181818, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=28.6764705882353, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788504, Length=359, Percent_Identity=25.9052924791086, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34442; Mature: 34442

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLGRYRFILQRPLQLLPVLFGISLVTFILVRSIPGDPARMLLGSRSTPQALANIRAQYG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHCC
LDEPLWTQYVYFMKNLVQGDMGKSLLYKIDVLKLISTRIEPTVFLVSGSVLLALLIAVPL
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
ATLAARNKGSWSDNLIRVFTTTGLGMPAFWLGIMLILLFSVQLGWFPVSGYGSDWLDKAH
HHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHH
HLVLPCLTIALALSAVLIRNLRASMLVELQADHVTAARARGLSEGALFRRHVLPNSLVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
INLLAVNIGWLISGTVVIESLFAIPGIGQLLVRGIFSRDYMVVQGVAMVLACATVIVNFL
CEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ADVATVAIDPRVNIK
HHHHHHEECCCCCCC
>Mature Secondary Structure
MNLGRYRFILQRPLQLLPVLFGISLVTFILVRSIPGDPARMLLGSRSTPQALANIRAQYG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHCC
LDEPLWTQYVYFMKNLVQGDMGKSLLYKIDVLKLISTRIEPTVFLVSGSVLLALLIAVPL
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
ATLAARNKGSWSDNLIRVFTTTGLGMPAFWLGIMLILLFSVQLGWFPVSGYGSDWLDKAH
HHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHH
HLVLPCLTIALALSAVLIRNLRASMLVELQADHVTAARARGLSEGALFRRHVLPNSLVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
INLLAVNIGWLISGTVVIESLFAIPGIGQLLVRGIFSRDYMVVQGVAMVLACATVIVNFL
CEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ADVATVAIDPRVNIK
HHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]