Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 66047408

GI number: 66047408

Start: 4974119

End: 4975600

Strand: Reverse

Name: nusA [H]

Synonym: Psyr_4181

Alternate gene names: 66047408

Gene position: 4975600-4974119 (Counterclockwise)

Preceding gene: 66047409

Following gene: 66047407

Centisome position: 81.65

GC content: 54.86

Gene sequence:

>1482_bases
ATGAGCAAAGAAGTACTGCTGGTTGTTGAGTCGGTGTCCAATGAAAAGGGCGTTCCGGCTAGTGTCATTTTTGAAGCGCT
GGAGCTGGCTCTGGCCACCGCTACCAAGAAGCGTTTTGAAGATGAAGTAGAACTGCGTGTAGAGATCAATCGCCAGACAG
GCAACTACGAGACTTTCCGTCGCTGGACAGTTGTCGAAGACGAAGATCTGGACGATCCTGCTTACGAATTGGCTGTTGAT
CAGGCCCAGGCGAAAAAGCCGGGTGCTGTTGCAGGCGACCTGATCGAAGAAAAAATCGATTCCATCGAATTCGGTCGTAT
CGCGGCGCAGACCGCCAAGCAAGTCATTGTGCAGAAAGTTCGCGAAGCCGAGCGTGCTCAGGTAGTCGATGCCTACCGCG
AGCGTCTGGGTGAAATCATTTCTGGTACTGTTAAAAAGGTTACACGTGACAATGTCATCGTTGACCTGGGTAACAATGCC
GAAGCCTTGCTGGCTCGTGAAGACATCATTTCCCGGGAAACTTTCCGGGTCGGTGTTCGCGTCCGTGCTCTGCTGAAAGA
AATTCGCACCGAAAACCGTGGCCCTCAGCTGATCCTGTCGCGTACTGCGCCAGAGATGCTGATCGAGCTTTTCCGCATTG
AAGTGCCGGAAATTGCCGAAGGTCTCATCGAGGTGATGGCTGCCTCCCGTGACCCGGGTTCTCGTGCGAAAATCGCTGTT
CGCTCCAAGGATAAACGAATCGACCCGCAAGGTGCCTGTATCGGCATGCGCGGTTCGCGAGTTCAGGCTGTTTCCGGCGA
GCTGGGTGGCGAGCGTGTGGATATCGTTCTCTGGGACGATAACCCGGCGCAGTTCGTGATCAATGCAATGTCCCCTGCAG
AAGTAGCGGCAATCATCGTAGATGAAGACGCGCATGCAATGGATATCGCAGTGGGAGCGGACAACCTGGCCCAGGCTATT
GGTCGTGGCGGTCAGAACGTGCGTCTGGCAAGTCAGCTGACTGGCTGGACCCTGAACGTAATGACCGAATCGGACATCCA
GGCCAAGCAGCAAGCTGAAACCGGTGACATCCTGCGCAACTTCATCGAAGAGCTCGAAGTCGATGAAGAATTGGCACAGG
TGCTGGTTGATGAAGGCTTCACCAGCCTGGAAGAGATTGCCTACGTACCGTTGGAAGAAATGCTCAACATCGACGGCTTT
GACGAAGACATCGTCAACGAGCTTCGCGCTCGTGCCAAGGATCGTTTGTTGACTAAAGCCATCGCTACAGAGGAAAAGCT
GGCAGACGCCCATCCGGCCGAAGACCTGCTCTCGCTTGAGGGTATGGACAAGGATTTGGCGATGGAACTGGCGGTGCGCG
GCGTAATTACCCGCGAAGACCTGGCCGAGCAGTCTATAGATGACCTGCTCGACATCGACGGCATTGACGATGATCGTGCC
GGCAAGTTGATCATGGCCGCCCGAGCCCACTGGTTCGAGTAA

Upstream 100 bases:

>100_bases
ACATGATCGACAAGGCCAACATTATTCCCACGTTTGACTGAGACGTGCCGGTTACTGCGGATCCCGCGGATCCAATGGCT
TGCGAAAGGCGAGGCGTACG

Downstream 100 bases:

>100_bases
TTGGGCGCGGCCTGAGGAGAGAAGTGCATGACGCAAGTCACGGTGAAAGAACTGGCCAAAGTGGTCGACACACCGGTAGA
GCGCCTGTTACAGCAGATGC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVELRVEINRQTGNYETFRRWTVVEDEDLDDPAYELAVD
QAQAKKPGAVAGDLIEEKIDSIEFGRIAAQTAKQVIVQKVREAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNA
EALLAREDIISRETFRVGVRVRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIVDEDAHAMDIAVGADNLAQAI
GRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRNFIEELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGF
DEDIVNELRARAKDRLLTKAIATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
GKLIMAARAHWFE

Sequences:

>Translated_493_residues
MSKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVELRVEINRQTGNYETFRRWTVVEDEDLDDPAYELAVD
QAQAKKPGAVAGDLIEEKIDSIEFGRIAAQTAKQVIVQKVREAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNA
EALLAREDIISRETFRVGVRVRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIVDEDAHAMDIAVGADNLAQAI
GRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRNFIEELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGF
DEDIVNELRARAKDRLLTKAIATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
GKLIMAARAHWFE
>Mature_492_residues
SKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVELRVEINRQTGNYETFRRWTVVEDEDLDDPAYELAVDQ
AQAKKPGAVAGDLIEEKIDSIEFGRIAAQTAKQVIVQKVREAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNAE
ALLAREDIISRETFRVGVRVRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAVR
SKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIVDEDAHAMDIAVGADNLAQAIG
RGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRNFIEELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGFD
EDIVNELRARAKDRLLTKAIATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRAG
KLIMAARAHWFE

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=493, Percent_Identity=63.6916835699797, Blast_Score=625, Evalue=1e-180,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54429; Mature: 54298

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS00037 MYB_1 ; PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVELRVEINRQTGNYETFR
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
RWTVVEDEDLDDPAYELAVDQAQAKKPGAVAGDLIEEKIDSIEFGRIAAQTAKQVIVQKV
EEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
REAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNAEALLAREDIISRETFRVGVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEE
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIV
ECCCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEEECCCCHHEEEECCCCCCEEEEEE
DEDAHAMDIAVGADNLAQAIGRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRN
ECCCCEEEEEECHHHHHHHHHCCCCCEEEHHHCCCCEEEEEECCHHHHHHHCHHHHHHHH
FIEELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGFDEDIVNELRARAKDRLLTKA
HHHHHCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GKLIMAARAHWFE
CCEEEEHHHHCCC
>Mature Secondary Structure 
SKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVELRVEINRQTGNYETFR
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
RWTVVEDEDLDDPAYELAVDQAQAKKPGAVAGDLIEEKIDSIEFGRIAAQTAKQVIVQKV
EEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
REAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNAEALLAREDIISRETFRVGVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEE
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIV
ECCCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEEECCCCHHEEEECCCCCCEEEEEE
DEDAHAMDIAVGADNLAQAIGRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRN
ECCCCEEEEEECHHHHHHHHHCCCCCEEEHHHCCCCEEEEEECCHHHHHHHCHHHHHHHH
FIEELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGFDEDIVNELRARAKDRLLTKA
HHHHHCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GKLIMAARAHWFE
CCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]