Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 66047381

GI number: 66047381

Start: 4940327

End: 4941673

Strand: Reverse

Name: pmbA [H]

Synonym: Psyr_4154

Alternate gene names: 66047381

Gene position: 4941673-4940327 (Counterclockwise)

Preceding gene: 66047383

Following gene: 66047380

Centisome position: 81.09

GC content: 62.73

Gene sequence:

>1347_bases
ATGAGTGCAACCCAAAGCGTCGGCCCACAAGCCTTGCCCGAGCTGCAGGAACAGGTCGAGCAGATCATTGCCGAAGCCCG
GCGTCAGGGCGCCAGCGCCTGCGAAGTCGCCGTTTCGGTGGACCAGGGGCTGTCGACCTCGGTCCGTCAGCGTGAAGTCG
AAACCGTCGAGTTCAACCGCGATCAGGGCTTTGGTATCACCCTGTATGTCGGACAGCGCAAGGGCTCGGCCAGCACGTCG
GCAACCGGTGCCGATGCCATTCGTGAAACGGTCGCTGCTGCACTGGCGATTGCCGAACACACCTCCGAAGATGAAAGCTC
CGGGCTGGCTGACGCTGCGCTGATGGCCAAAGAGGTCATGGATTTTGACCTTTATCACACCTGGGACATCACGCCCGAGC
AGGCCATCGAGAAAGCGCTGCTCTGCGAAGCGGCGGCCTTCGATGCCGACAGCCGCATCAAGAATGCCGACGGCACTACG
CTCAATACCCATCAGGGCTGCCGTGTGTACGGCAACAGCAATGGCTTCATCGGTGGCTATGCGTCAACGCGGCACAGCCT
CAGCTGTGTGATGATCGCCGAGGGCGAAGGTCAGATGCAGCGCGATTACTGGTATGACGTCAACCGCCAGGGCGAGCTGC
TGGCCGATGCGCAGAGCATTGGTCGCAAGGCGGCGTTGCGTGCAGCGAGCCGTCTGGGCGCGCGTCCTGTGCCGACCTGT
GAAGTGCCGGTGCTGTTTGCTGCAGAGCTGGCAGGCGGCCTGTTCGGCAGCTTTCTCGGCGCCATTTCCGGGGGTACCCT
GTATCGCAAGTCGTCGTTCCTCGAAGGCGCGCTGGGTCAGCGCCTGTTCCCTGAGTGGATGACCATTGATGAGCGCCCGC
ACCTCAGGCATGCGATGGGCAGCTCGGCGTTCGACAGCGATGGTCTGGCGACCTTCGCCAAACCGTTCGTGGCAGGTGGC
GATCTGGTTTCCTATGTGCTGAGCACGTATTCCGGCCGCAAGCTGGGCATGCCGAGCACCGCCAACGCGGGCGGTGTGCA
CAACCTGTTCGTTAGCCATGGCTCGGACGACCAGGCTGCGCTGATCCGGCGCATGGGACGTGGCCTGCTGGTGACCGAAT
TGATGGGCAGCGGTCTGAACATGGTCACCGGCGACTATTCACGCGGCGCGGCAGGCTTCTGGGTCGAGAACGGCGAGATT
CAGTTCCCGGTTCAGGAAGTGACCATTGCCGGCAACATGCGCGACATGTTCAAGCAGATCGTCGCCGTTGGCAGCGATCT
TGAGCTGCGCAGCAACATCCGCACCGGCTCGGTACTGATCGAGCGCATGATGGTGGCGGGGAGCTGA

Upstream 100 bases:

>100_bases
GTCCGCAAAGAAACGCCGCCATGATAACCAGTCGTGGGCCGCTTGTCCGGCCCGGTCGGTATTCGGCCTGTGCTGAAAGA
AGAATTTGATGGAGAAAGTC

Downstream 100 bases:

>100_bases
CAGATCCCCGATTCAGGCTATCGTTCCTCACGCTCCCGCGTGTGAATGCAGTTCACGACGCTCTGCGTCGTCAAGAGGAC
GCAGAGCGTCCGGAAAGGCA

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSATQSVGPQALPELQEQVEQIIAEARRQGASACEVAVSVDQGLSTSVRQREVETVEFNRDQGFGITLYVGQRKGSASTS
ATGADAIRETVAAALAIAEHTSEDESSGLADAALMAKEVMDFDLYHTWDITPEQAIEKALLCEAAAFDADSRIKNADGTT
LNTHQGCRVYGNSNGFIGGYASTRHSLSCVMIAEGEGQMQRDYWYDVNRQGELLADAQSIGRKAALRAASRLGARPVPTC
EVPVLFAAELAGGLFGSFLGAISGGTLYRKSSFLEGALGQRLFPEWMTIDERPHLRHAMGSSAFDSDGLATFAKPFVAGG
DLVSYVLSTYSGRKLGMPSTANAGGVHNLFVSHGSDDQAALIRRMGRGLLVTELMGSGLNMVTGDYSRGAAGFWVENGEI
QFPVQEVTIAGNMRDMFKQIVAVGSDLELRSNIRTGSVLIERMMVAGS

Sequences:

>Translated_448_residues
MSATQSVGPQALPELQEQVEQIIAEARRQGASACEVAVSVDQGLSTSVRQREVETVEFNRDQGFGITLYVGQRKGSASTS
ATGADAIRETVAAALAIAEHTSEDESSGLADAALMAKEVMDFDLYHTWDITPEQAIEKALLCEAAAFDADSRIKNADGTT
LNTHQGCRVYGNSNGFIGGYASTRHSLSCVMIAEGEGQMQRDYWYDVNRQGELLADAQSIGRKAALRAASRLGARPVPTC
EVPVLFAAELAGGLFGSFLGAISGGTLYRKSSFLEGALGQRLFPEWMTIDERPHLRHAMGSSAFDSDGLATFAKPFVAGG
DLVSYVLSTYSGRKLGMPSTANAGGVHNLFVSHGSDDQAALIRRMGRGLLVTELMGSGLNMVTGDYSRGAAGFWVENGEI
QFPVQEVTIAGNMRDMFKQIVAVGSDLELRSNIRTGSVLIERMMVAGS
>Mature_447_residues
SATQSVGPQALPELQEQVEQIIAEARRQGASACEVAVSVDQGLSTSVRQREVETVEFNRDQGFGITLYVGQRKGSASTSA
TGADAIRETVAAALAIAEHTSEDESSGLADAALMAKEVMDFDLYHTWDITPEQAIEKALLCEAAAFDADSRIKNADGTTL
NTHQGCRVYGNSNGFIGGYASTRHSLSCVMIAEGEGQMQRDYWYDVNRQGELLADAQSIGRKAALRAASRLGARPVPTCE
VPVLFAAELAGGLFGSFLGAISGGTLYRKSSFLEGALGQRLFPEWMTIDERPHLRHAMGSSAFDSDGLATFAKPFVAGGD
LVSYVLSTYSGRKLGMPSTANAGGVHNLFVSHGSDDQAALIRRMGRGLLVTELMGSGLNMVTGDYSRGAAGFWVENGEIQ
FPVQEVTIAGNMRDMFKQIVAVGSDLELRSNIRTGSVLIERMMVAGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=425, Percent_Identity=49.6470588235294, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1789640, Length=412, Percent_Identity=20.873786407767, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47773; Mature: 47642

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATQSVGPQALPELQEQVEQIIAEARRQGASACEVAVSVDQGLSTSVRQREVETVEFNR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHEEEECC
DQGFGITLYVGQRKGSASTSATGADAIRETVAAALAIAEHTSEDESSGLADAALMAKEVM
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
DFDLYHTWDITPEQAIEKALLCEAAAFDADSRIKNADGTTLNTHQGCRVYGNSNGFIGGY
HCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCCCEEECC
ASTRHSLSCVMIAEGEGQMQRDYWYDVNRQGELLADAQSIGRKAALRAASRLGARPVPTC
CCCCCCEEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EVPVLFAAELAGGLFGSFLGAISGGTLYRKSSFLEGALGQRLFPEWMTIDERPHLRHAMG
CCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHC
SSAFDSDGLATFAKPFVAGGDLVSYVLSTYSGRKLGMPSTANAGGVHNLFVSHGSDDQAA
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
LIRRMGRGLLVTELMGSGLNMVTGDYSRGAAGFWVENGEIQFPVQEVTIAGNMRDMFKQI
HHHHHCCCEEHHHHHCCCCCEEECCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHH
VAVGSDLELRSNIRTGSVLIERMMVAGS
HHCCCCCHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SATQSVGPQALPELQEQVEQIIAEARRQGASACEVAVSVDQGLSTSVRQREVETVEFNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHEEEECC
DQGFGITLYVGQRKGSASTSATGADAIRETVAAALAIAEHTSEDESSGLADAALMAKEVM
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
DFDLYHTWDITPEQAIEKALLCEAAAFDADSRIKNADGTTLNTHQGCRVYGNSNGFIGGY
HCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCCCEEECC
ASTRHSLSCVMIAEGEGQMQRDYWYDVNRQGELLADAQSIGRKAALRAASRLGARPVPTC
CCCCCCEEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EVPVLFAAELAGGLFGSFLGAISGGTLYRKSSFLEGALGQRLFPEWMTIDERPHLRHAMG
CCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHC
SSAFDSDGLATFAKPFVAGGDLVSYVLSTYSGRKLGMPSTANAGGVHNLFVSHGSDDQAA
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
LIRRMGRGLLVTELMGSGLNMVTGDYSRGAAGFWVENGEIQFPVQEVTIAGNMRDMFKQI
HHHHHCCCEEHHHHHCCCCCEEECCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHH
VAVGSDLELRSNIRTGSVLIERMMVAGS
HHCCCCCHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]