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The map label for this gene is mlaE [H]

Identifier: 66047367

GI number: 66047367

Start: 4929128

End: 4929925

Strand: Reverse

Name: mlaE [H]

Synonym: Psyr_4140

Alternate gene names: 66047367

Gene position: 4929925-4929128 (Counterclockwise)

Preceding gene: 66047368

Following gene: 66047366

Centisome position: 80.9

GC content: 57.52

Gene sequence:

>798_bases
ATGCGCAATAAGTCACTCATGGACCGGGTTCGCCTGCTGGGGCGCTCGGCCATTGATATTGTTACTGTTCTGGGGCGTTC
CGGTCTGTTTCTGGTGCATGCCTTGCTCGGTCGTGGCGGTGCGGGCAGCAGTTTCCAACTGCTGGTCAGGCAGCTGTATT
CGGTTGGCGTCATGTCGCTGGTCATCATTGTGGTGTCCGGCATGTTCATCGGCATGGTGCTGGCGCTGCAGGGCTTCAGC
ATCCTGACCAAATACGGTTCGGAGCAGGCGGTTGGCCAGATGGTTGCCCTGACGCTGTTGCGTGAACTCGGCCCTGTGGT
GACGGCCTTGCTGTTCGCCGGTCGTGCGGGCTCTGCGTTGACCGCCGAGATCGGCAACATGAAGTCCACCGAGCAGCTGT
CGAGCCTTGAAATGATCGGTGTCGATCCGCTCAAGTACATCGTGGCGCCGCGTCTGTGGGCCGGGTTCATCTCGCTGCCG
ATTCTGGCGATGATCTTCAGCGTGGTCGGTATCTGGGGCGCTTCCTGGGTGGCGATCGATTGGCTGGGAGTCTATGACGG
TTCTTTCTGGTCGAACATGCAGAATAGCGTTTCTTTCACGAACGATGTGCTCAATGGGGTGATCAAAAGCGTCGTATTTG
CCTTCGTGGTGACATGGATTGCCGTTTTCCAGGGTTACGATTGCGAGCCTACTTCCGAGGGGATCAGTCGCGCGACGACC
AGAACCGTCGTTTACGCCTCGTTGGCCGTGCTGGGACTGGACTTTATTTTGACCGCTTTGATGTTTGGAGATTTCTGA

Upstream 100 bases:

>100_bases
ATCCACGTATTCGTCAATTCATGACCGGTGAACCGGACGGCCCTGTACCGTTCCACTATCCGGCGCCGGATTTTCGCGAA
GATCTATTGGGGAAGCGCTG

Downstream 100 bases:

>100_bases
TGCAAAACCGTGCCATCGAAACCGGAGTCGGTCTGTTCCTGCTGGCGGGGATACTGGCTCTGCTCCTGCTGGCCCTGCGA
GTCAGTGGTCTGAGCACAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRNKSLMDRVRLLGRSAIDIVTVLGRSGLFLVHALLGRGGAGSSFQLLVRQLYSVGVMSLVIIVVSGMFIGMVLALQGFS
ILTKYGSEQAVGQMVALTLLRELGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLEMIGVDPLKYIVAPRLWAGFISLP
ILAMIFSVVGIWGASWVAIDWLGVYDGSFWSNMQNSVSFTNDVLNGVIKSVVFAFVVTWIAVFQGYDCEPTSEGISRATT
RTVVYASLAVLGLDFILTALMFGDF

Sequences:

>Translated_265_residues
MRNKSLMDRVRLLGRSAIDIVTVLGRSGLFLVHALLGRGGAGSSFQLLVRQLYSVGVMSLVIIVVSGMFIGMVLALQGFS
ILTKYGSEQAVGQMVALTLLRELGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLEMIGVDPLKYIVAPRLWAGFISLP
ILAMIFSVVGIWGASWVAIDWLGVYDGSFWSNMQNSVSFTNDVLNGVIKSVVFAFVVTWIAVFQGYDCEPTSEGISRATT
RTVVYASLAVLGLDFILTALMFGDF
>Mature_265_residues
MRNKSLMDRVRLLGRSAIDIVTVLGRSGLFLVHALLGRGGAGSSFQLLVRQLYSVGVMSLVIIVVSGMFIGMVLALQGFS
ILTKYGSEQAVGQMVALTLLRELGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLEMIGVDPLKYIVAPRLWAGFISLP
ILAMIFSVVGIWGASWVAIDWLGVYDGSFWSNMQNSVSFTNDVLNGVIKSVVFAFVVTWIAVFQGYDCEPTSEGISRATT
RTVVYASLAVLGLDFILTALMFGDF

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=62.1621621621622, Blast_Score=319, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28478; Mature: 28478

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]