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The map label for this gene is mscS [H]

Identifier: 66047313

GI number: 66047313

Start: 4872023

End: 4872853

Strand: Reverse

Name: mscS [H]

Synonym: Psyr_4086

Alternate gene names: 66047313

Gene position: 4872853-4872023 (Counterclockwise)

Preceding gene: 66047314

Following gene: 66047303

Centisome position: 79.97

GC content: 62.45

Gene sequence:

>831_bases
ATGGATTTAAACGCAGAAGTCGGCCATCTGTGGCAAGCGTCGCAGGCCTGGATTCCGATGATCATGGAGTACGGCAGCCG
CTTGCTGCTGGCAGTCGTCACACTGTGCGTAGGCTGGTGGCTGATCAACCGGCTGACCGGCAAACTGGGCGCGTTGCTGG
CCTTGCGACACGCCGATCTGGCCTTGCAGGGCTTCGTCAGCAACCTGGCCAACGTCATTCTGAAAATCCTGCTGGTGGTC
AGCGTCGCGTCGATGATCGGCGTGGAAACCACCTCGTTCGTGGCGGCGATCGGTGCGGCCGGTCTGGCGATCGGTCTGGC
CCTGCAGGGCAGCCTGGCGAACTTCGCCGGTGGCGTGCTGATTCTGTTGTTCCGCCCGTTCCGTATCGGTGACTGGATCG
AAGCCCAGGGTGTGTCGGGAACCGTCGACAACATCCAGATCTTCCACACCGTGCTGCGCACCGGCGACAACCGCACCGTG
ATCATTCCCAACGGCAACCTGTCCAACGGCATCATCACCAACACCAACCGTCAGCCGACCCGCAAGATCACCTTCGACAT
CGGCGTCAGCCACGACGCCGATCTCAAGAAGGCCTTGCAGGTCCTGCTGGACATGGCCGACGACCCGCGCGTCCTCAAGG
ACCCGGCCCCCCAGGCTGTGGTGGCTGCGCTGGGGGAGAACGCAATTACCTTGTCCCTGCGTGTCTGGACCAGCAGTGGC
GACATGGGCGACGTGACGTCGATGTTCAACATCGAAGCGCGCGACCGCCTGAAGGACGCAGGCATCGAGATCCCACTGCC
GCAACGGATTGTGCGGGTGGTTCAGGAATAG

Upstream 100 bases:

>100_bases
AACCCTGCGTTCCCCGGCCAGTCCGAATCGCCCTGAGCATTGAAATGACGGCAGGCCGCCGTGGTTTTGATCGCAGTTCG
TGACGAGTAAGGAGATAACG

Downstream 100 bases:

>100_bases
TGGTTTGTGCCGTAAGACAAAGCCCTGGCTCGTGAGAGTCGGGGCTTTTCTTTATTTACAGGCTCAGGAAAAGGCTATTT
CCATGGTTGATGATTGTCTG

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDLNAEVGHLWQASQAWIPMIMEYGSRLLLAVVTLCVGWWLINRLTGKLGALLALRHADLALQGFVSNLANVILKILLVV
SVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVLILLFRPFRIGDWIEAQGVSGTVDNIQIFHTVLRTGDNRTV
IIPNGNLSNGIITNTNRQPTRKITFDIGVSHDADLKKALQVLLDMADDPRVLKDPAPQAVVAALGENAITLSLRVWTSSG
DMGDVTSMFNIEARDRLKDAGIEIPLPQRIVRVVQE

Sequences:

>Translated_276_residues
MDLNAEVGHLWQASQAWIPMIMEYGSRLLLAVVTLCVGWWLINRLTGKLGALLALRHADLALQGFVSNLANVILKILLVV
SVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVLILLFRPFRIGDWIEAQGVSGTVDNIQIFHTVLRTGDNRTV
IIPNGNLSNGIITNTNRQPTRKITFDIGVSHDADLKKALQVLLDMADDPRVLKDPAPQAVVAALGENAITLSLRVWTSSG
DMGDVTSMFNIEARDRLKDAGIEIPLPQRIVRVVQE
>Mature_276_residues
MDLNAEVGHLWQASQAWIPMIMEYGSRLLLAVVTLCVGWWLINRLTGKLGALLALRHADLALQGFVSNLANVILKILLVV
SVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVLILLFRPFRIGDWIEAQGVSGTVDNIQIFHTVLRTGDNRTV
IIPNGNLSNGIITNTNRQPTRKITFDIGVSHDADLKKALQVLLDMADDPRVLKDPAPQAVVAALGENAITLSLRVWTSSG
DMGDVTSMFNIEARDRLKDAGIEIPLPQRIVRVVQE

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=257, Percent_Identity=36.1867704280156, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1786670, Length=219, Percent_Identity=31.9634703196347, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787591, Length=176, Percent_Identity=27.8409090909091, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367355, Length=220, Percent_Identity=25.9090909090909, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29688; Mature: 29688

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNAEVGHLWQASQAWIPMIMEYGSRLLLAVVTLCVGWWLINRLTGKLGALLALRHADL
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQGFVSNLANVILKILLVVSVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEE
ILLFRPFRIGDWIEAQGVSGTVDNIQIFHTVLRTGDNRTVIIPNGNLSNGIITNTNRQPT
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCC
RKITFDIGVSHDADLKKALQVLLDMADDPRVLKDPAPQAVVAALGENAITLSLRVWTSSG
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCC
DMGDVTSMFNIEARDRLKDAGIEIPLPQRIVRVVQE
CCCCHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MDLNAEVGHLWQASQAWIPMIMEYGSRLLLAVVTLCVGWWLINRLTGKLGALLALRHADL
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQGFVSNLANVILKILLVVSVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEE
ILLFRPFRIGDWIEAQGVSGTVDNIQIFHTVLRTGDNRTVIIPNGNLSNGIITNTNRQPT
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCC
RKITFDIGVSHDADLKKALQVLLDMADDPRVLKDPAPQAVVAALGENAITLSLRVWTSSG
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCC
DMGDVTSMFNIEARDRLKDAGIEIPLPQRIVRVVQE
CCCCHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]