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The map label for this gene is fumA [H]

Identifier: 66047258

GI number: 66047258

Start: 4799474

End: 4800997

Strand: Direct

Name: fumA [H]

Synonym: Psyr_4031

Alternate gene names: 66047258

Gene position: 4799474-4800997 (Clockwise)

Preceding gene: 66047256

Following gene: 66047261

Centisome position: 78.76

GC content: 62.01

Gene sequence:

>1524_bases
ATGACCGTGATCAAGCAAGACGACCTGATTCAGAGCGTTGCAGACGCACTGCAGTTCATTTCCTATTACCACCCCGTGGA
CTTCATCCAGGCCATGCACGAGGCGTATCTGCGTGAAGAGTCGCCTGCCGCTCGTGACTCCATCGCCCAGATCCTGATCA
ACTCGCGCATGTGCGCGACCGGTCATCGCCCGATCTGCCAGGACACCGGCATCGTCACGGTTTTCGTGCGCGTGGGGATG
GATGTTCGCTGGGACGGCGCCACCATGGGCCTGGACGACATGATCAACGAAGGCGTGCGTCGCGCGTACAACCTGCCTGA
AAACGTGCTGCGTGCCTCGATCCTTGCCGATCCGGCAGGCGCTCGCAAAAACACCAAGGACAACACCCCGGCCGTCATTC
ACTACTCCATCGTCCCGGGTAACACCGTGGAAGTGGACGTCGCGGCGAAAGGCGGCGGTTCGGAAAACAAATCGAAGATG
GCGATGCTCAACCCGTCCGATTCGATCGTCGACTGGGTTCTGAAAACCGTACCGACCATGGGCGCAGGCTGGTGCCCGCC
GGGCATGCTCGGCATCGGCATCGGCGGTACGGCTGAAAAAGCCGCCGTTATGGCCAAGGAAGTGTTGATGGAGTCCATCG
ACATTCATGAACTTAAAGCCCGCGGCCCGCAGAATCGTATCGAAGAGATGCGTCTGGAGCTGTTCGAGAAGGTCAACCAG
CTGGGCATTGGCGCTCAGGGCCTGGGCGGTCTGACCACCGTGCTCGACGTCAAGATCATGGATTACCCGACCCACGCCGC
GTCGCTGCCGGTGTGCATGATCCCCAATTGCGCCGCCACCCGCCACGCGCACTTCGTGCTCGACGGCAGCGGCCCTGCTT
CGCTGGAGGCGCCATCGCTGGACGCCTACCCGGAAATCGTCTGGGAAGCCGGCCCATCGGCCCGCCGCGTCAACCTCGAT
ACGCTGACCCCGGAAGAAGTACAAAGCTGGAAACCGGGCGAAACCGTTTTGCTCAATGGCAAGATGCTCACCGGCCGCGA
CGCGGCGCACAAGCGCATGGTCGAAATGCTCAACCGGGGTGAAACCCTGCCGGTAGACCTCAAGGGTCGCTTCATCTACT
ACGTCGGCCCGGTTGATCCGGTGCGCGAAGAAGTCGTCGGCCCGGCCGGCCCGACCACGGCCACCCGGATGGACAAGTTC
ACCCGGCAGATCCTCGACCAGACCGGCCTGCTGGGCATGATCGGCAAATCCGAACGCGGCCCGACCGCCATCGAAGCGAT
CCGTGACCACAAGGCCGTGTACCTGATGGCCGTCGGTGGTGCTGCCTATTTGGTGGCTCAGGCGATCAAGAAATCCCGCG
TCGTGGCCTTCGCCGAGCTGGGCATGGAAGCGATCTACGAGTTCGAGGTCAAGGACATGCCGGTGACCGTGGCCGTAGAC
AGCAACGGTGAGTCCGTACACATCACCGGCCCGCAGATCTGGCAGAAGAAGATCAGCGACAGTCTGGCGGTGGAAGTGCA
GTAA

Upstream 100 bases:

>100_bases
CCCGCTATCGCCAACCTGACAGCTCTGGTTCAAGCCGCAGTTCCAGACTGCCGCCGATAGCGTAATTGAATGTTCCCGTC
CTTACAGAGGAGCGCGCCGC

Downstream 100 bases:

>100_bases
GCACCGGATAAAACCCGTCTCATTGAGGCGGGTTTTATCGTTTATGGTCAGCGCCAGGTTTGCGCCGCGCCGCTTTTTAT
AGGCCGATGCAGCGGCATCC

Product: Fe-S type hydro-lyase tartrate/fumarate alpha region:Fe-S type hydro-lyase tartrate/fumarate beta region

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSIAQILINSRMCATGHRPICQDTGIVTVFVRVGM
DVRWDGATMGLDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKM
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPSLDAYPEIVWEAGPSARRVNLD
TLTPEEVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNRGETLPVDLKGRFIYYVGPVDPVREEVVGPAGPTTATRMDKF
TRQILDQTGLLGMIGKSERGPTAIEAIRDHKAVYLMAVGGAAYLVAQAIKKSRVVAFAELGMEAIYEFEVKDMPVTVAVD
SNGESVHITGPQIWQKKISDSLAVEVQ

Sequences:

>Translated_507_residues
MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSIAQILINSRMCATGHRPICQDTGIVTVFVRVGM
DVRWDGATMGLDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKM
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPSLDAYPEIVWEAGPSARRVNLD
TLTPEEVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNRGETLPVDLKGRFIYYVGPVDPVREEVVGPAGPTTATRMDKF
TRQILDQTGLLGMIGKSERGPTAIEAIRDHKAVYLMAVGGAAYLVAQAIKKSRVVAFAELGMEAIYEFEVKDMPVTVAVD
SNGESVHITGPQIWQKKISDSLAVEVQ
>Mature_506_residues
TVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSIAQILINSRMCATGHRPICQDTGIVTVFVRVGMD
VRWDGATMGLDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKMA
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQL
GIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPSLDAYPEIVWEAGPSARRVNLDT
LTPEEVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNRGETLPVDLKGRFIYYVGPVDPVREEVVGPAGPTTATRMDKFT
RQILDQTGLLGMIGKSERGPTAIEAIRDHKAVYLMAVGGAAYLVAQAIKKSRVVAFAELGMEAIYEFEVKDMPVTVAVDS
NGESVHITGPQIWQKKISDSLAVEVQ

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=468, Percent_Identity=33.974358974359, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1790564, Length=451, Percent_Identity=32.8159645232816, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789443, Length=169, Percent_Identity=33.1360946745562, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789442, Length=285, Percent_Identity=28.0701754385965, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54923; Mature: 54792

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSIAQILINSRMCAT
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECC
GHRPICQDTGIVTVFVRVGMDVRWDGATMGLDDMINEGVRRAYNLPENVLRASILADPAG
CCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
ARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKMAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPSL
HCCCCCCCCCCEEEEEEEEEECCCCHHCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCC
DAYPEIVWEAGPSARRVNLDTLTPEEVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNRG
CCCCHHHCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHCC
ETLPVDLKGRFIYYVGPVDPVREEVVGPAGPTTATRMDKFTRQILDQTGLLGMIGKSERG
CCCEEEECCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCC
PTAIEAIRDHKAVYLMAVGGAAYLVAQAIKKSRVVAFAELGMEAIYEFEVKDMPVTVAVD
CHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEEEC
SNGESVHITGPQIWQKKISDSLAVEVQ
CCCCEEEECCHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSIAQILINSRMCAT
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECC
GHRPICQDTGIVTVFVRVGMDVRWDGATMGLDDMINEGVRRAYNLPENVLRASILADPAG
CCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
ARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKMAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPSL
HCCCCCCCCCCEEEEEEEEEECCCCHHCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCC
DAYPEIVWEAGPSARRVNLDTLTPEEVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNRG
CCCCHHHCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHCC
ETLPVDLKGRFIYYVGPVDPVREEVVGPAGPTTATRMDKFTRQILDQTGLLGMIGKSERG
CCCEEEECCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCC
PTAIEAIRDHKAVYLMAVGGAAYLVAQAIKKSRVVAFAELGMEAIYEFEVKDMPVTVAVD
CHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEEEC
SNGESVHITGPQIWQKKISDSLAVEVQ
CCCCEEEECCHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]