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The map label for this gene is pykA [H]

Identifier: 66047256

GI number: 66047256

Start: 4796791

End: 4798242

Strand: Direct

Name: pykA [H]

Synonym: Psyr_4029

Alternate gene names: 66047256

Gene position: 4796791-4798242 (Clockwise)

Preceding gene: 66047253

Following gene: 66047258

Centisome position: 78.72

GC content: 62.12

Gene sequence:

>1452_bases
ATGACCGTTCGCCGCACCAAAATCGTCGCCACACTTGGCCCTGCCAGCAACTCGCCGGAAGTCATCGAACAACTGATCCT
TTCGGGTCTGGACGTTGCTCGTCTGAACTTCTCTCACGGTACTCCGGACGAGCACAAGGCTCGCGCCAGGCTGATCCGCG
AAATCGCCGCCAGACACGGGCGCTTCGTCGCTCTGCTGGGCGACCTGCAGGGTCCGAAGATTCGTATCGCAAAGTTCACC
GACAAGCGAATAGAGCTGAAGGTCGGTGACAAGTTCACCTTCTCCACTGCTCATCCGCTGACCAGTGGCAACCAGCAGAT
CGTGGGTATCGATTACCCGGATCTGGTCAAGGATTGCGGCGTAGGCGACGAGTTGCTGCTCGACGATGGTCGCGTGGTCA
TGCGCGTCGACACCCAGACCGCTGACGAACTGCACTGCACCGTGCTGATCGGTGGTCCGCTGTCCGACCACAAAGGCATC
AACCGTCGTGGTGGTGGCCTGACGGCGCCAGCACTGACCGAAAAAGACAAGCAGGACATCAAGCTCGCTGCCGAGATGGA
CCTGGACTACCTGGCCGTTTCCTTCCCGCGCGACGCCGCCGACATGGAATACGCCCGCCAGTTGCGCGACGAATCCGGTG
GTACGGCCTGGCTGGTGGCCAAGATCGAACGTGCCGAAGCCGTTGCCAACGACGAAGTGCTGGATGAGCTGATCCGCGCC
AGTGACGCGGTCATGGTCGCTCGTGGTGACCTGGGTGTCGAAATCGGTGATGCCGAACTGGTCGGCGTACAGAAACGCAT
CATCCTGCACGCCCGTCGCCACAACAAGGCGGTGATCGTCGCCACGCAGATGATGGAGTCGATGATCTCCAGCCCCATGC
CGACGCGTGCCGAAGTGTCCGACGTGGCCAACGCCGTGCTCGACTACACCGACGCCGTGATGCTGTCGGCCGAAAGTGCT
GCAGGCTCCTACCCGATCGAAGCGGTTCAGGCCATGGCCCGTATCTGCATCGGTGCTGAAAAGCACCCGACCGGCAAGAC
GTCCAGCCACCGCATCGGTCACTCGTTCACCCGTTGTGACGAAAGCATCGCGCTGGCGGCCATGTACACCGCCAACCACT
TCCCGGGTGTGAAGGCGATCATCGCACTGACCGAAAGCGGCTACACCCCGCTGATCATGTCGCGCATCCGCTCCTCGGTG
CCGATCTACGCGTTCTCTCCGCACCGTGGTACTCAGGCGCGTGCTGCGATGTTCCGTGGCGTCTACACCGTGCCATTCGA
CCCGGCAGCGCTGCCGCCAGGTCAGGTCAGCCAGGCCGCAGTCGACGAACTGCTCAAGCGTGGTCTGGTCGAGCAAGGTG
ACTGGGTCATCCTCACCAAGGGCGACAGCTACCACACCATCGGCGGCACCAACGGCATGAAGATTCTGCACGTCGGCGAC
CCGTTGGTCTGA

Upstream 100 bases:

>100_bases
GATTGCCCTCCCTGACCCTGACACGGTAGTGCAAAGGAACAATGACTAAAGACCCAGACAGGAGTAGCCTCTCGCTGCGC
TTCAATTAAGGAGTTTGTGC

Downstream 100 bases:

>100_bases
TCGCAGCACGCTGCAAGCCAAGGCCCCGTCTCGCAAGAGTCGGGGCCTTTTGCGTTGGGTCAGCCTTTTGTGATTATCGT
GTCGAAGAACGAGAACTGTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTVRRTKIVATLGPASNSPEVIEQLILSGLDVARLNFSHGTPDEHKARARLIREIAARHGRFVALLGDLQGPKIRIAKFT
DKRIELKVGDKFTFSTAHPLTSGNQQIVGIDYPDLVKDCGVGDELLLDDGRVVMRVDTQTADELHCTVLIGGPLSDHKGI
NRRGGGLTAPALTEKDKQDIKLAAEMDLDYLAVSFPRDAADMEYARQLRDESGGTAWLVAKIERAEAVANDEVLDELIRA
SDAVMVARGDLGVEIGDAELVGVQKRIILHARRHNKAVIVATQMMESMISSPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGSYPIEAVQAMARICIGAEKHPTGKTSSHRIGHSFTRCDESIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSV
PIYAFSPHRGTQARAAMFRGVYTVPFDPAALPPGQVSQAAVDELLKRGLVEQGDWVILTKGDSYHTIGGTNGMKILHVGD
PLV

Sequences:

>Translated_483_residues
MTVRRTKIVATLGPASNSPEVIEQLILSGLDVARLNFSHGTPDEHKARARLIREIAARHGRFVALLGDLQGPKIRIAKFT
DKRIELKVGDKFTFSTAHPLTSGNQQIVGIDYPDLVKDCGVGDELLLDDGRVVMRVDTQTADELHCTVLIGGPLSDHKGI
NRRGGGLTAPALTEKDKQDIKLAAEMDLDYLAVSFPRDAADMEYARQLRDESGGTAWLVAKIERAEAVANDEVLDELIRA
SDAVMVARGDLGVEIGDAELVGVQKRIILHARRHNKAVIVATQMMESMISSPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGSYPIEAVQAMARICIGAEKHPTGKTSSHRIGHSFTRCDESIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSV
PIYAFSPHRGTQARAAMFRGVYTVPFDPAALPPGQVSQAAVDELLKRGLVEQGDWVILTKGDSYHTIGGTNGMKILHVGD
PLV
>Mature_482_residues
TVRRTKIVATLGPASNSPEVIEQLILSGLDVARLNFSHGTPDEHKARARLIREIAARHGRFVALLGDLQGPKIRIAKFTD
KRIELKVGDKFTFSTAHPLTSGNQQIVGIDYPDLVKDCGVGDELLLDDGRVVMRVDTQTADELHCTVLIGGPLSDHKGIN
RRGGGLTAPALTEKDKQDIKLAAEMDLDYLAVSFPRDAADMEYARQLRDESGGTAWLVAKIERAEAVANDEVLDELIRAS
DAVMVARGDLGVEIGDAELVGVQKRIILHARRHNKAVIVATQMMESMISSPMPTRAEVSDVANAVLDYTDAVMLSAESAA
GSYPIEAVQAMARICIGAEKHPTGKTSSHRIGHSFTRCDESIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSVP
IYAFSPHRGTQARAAMFRGVYTVPFDPAALPPGQVSQAAVDELLKRGLVEQGDWVILTKGDSYHTIGGTNGMKILHVGDP
LV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=497, Percent_Identity=37.2233400402414, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI33286420, Length=497, Percent_Identity=37.2233400402414, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI33286418, Length=497, Percent_Identity=37.2233400402414, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI10835121, Length=497, Percent_Identity=34.4064386317907, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI32967597, Length=497, Percent_Identity=34.4064386317907, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI310128732, Length=293, Percent_Identity=37.8839590443686, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI310128730, Length=293, Percent_Identity=37.8839590443686, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=36.4016736401674, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=36.4016736401674, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=35.3488372093023, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=55.8823529411765, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1787965, Length=462, Percent_Identity=37.2294372294372, Blast_Score=284, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=502, Percent_Identity=36.2549800796813, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=36.1396303901437, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=36.1396303901437, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=36.1396303901437, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=492, Percent_Identity=36.1788617886179, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=489, Percent_Identity=33.9468302658487, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=494, Percent_Identity=32.9959514170041, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=35.9756097560976, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=35.9756097560976, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=37.8048780487805, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=30.7535641547862, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24646914, Length=279, Percent_Identity=37.9928315412186, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52152; Mature: 52021

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRRTKIVATLGPASNSPEVIEQLILSGLDVARLNFSHGTPDEHKARARLIREIAARHG
CCCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
RFVALLGDLQGPKIRIAKFTDKRIELKVGDKFTFSTAHPLTSGNQQIVGIDYPDLVKDCG
CEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCEEEECCCHHHHHHCC
VGDELLLDDGRVVMRVDTQTADELHCTVLIGGPLSDHKGINRRGGGLTAPALTEKDKQDI
CCCEEEECCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHE
KLAAEMDLDYLAVSFPRDAADMEYARQLRDESGGTAWLVAKIERAEAVANDEVLDELIRA
EEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHCCHHHHHHHHHC
SDAVMVARGDLGVEIGDAELVGVQKRIILHARRHNKAVIVATQMMESMISSPMPTRAEVS
CCEEEEEECCCCEEECCCCEEHHHHHHHEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDYTDAVMLSAESAAGSYPIEAVQAMARICIGAEKHPTGKTSSHRIGHSFTRCD
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCC
ESIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSVPIYAFSPHRGTQARAAMFRG
CHHEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCC
VYTVPFDPAALPPGQVSQAAVDELLKRGLVEQGDWVILTKGDSYHTIGGTNGMKILHVGD
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCEEEEECCC
PLV
CCC
>Mature Secondary Structure 
TVRRTKIVATLGPASNSPEVIEQLILSGLDVARLNFSHGTPDEHKARARLIREIAARHG
CCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
RFVALLGDLQGPKIRIAKFTDKRIELKVGDKFTFSTAHPLTSGNQQIVGIDYPDLVKDCG
CEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCEEEECCCHHHHHHCC
VGDELLLDDGRVVMRVDTQTADELHCTVLIGGPLSDHKGINRRGGGLTAPALTEKDKQDI
CCCEEEECCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHE
KLAAEMDLDYLAVSFPRDAADMEYARQLRDESGGTAWLVAKIERAEAVANDEVLDELIRA
EEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHCCHHHHHHHHHC
SDAVMVARGDLGVEIGDAELVGVQKRIILHARRHNKAVIVATQMMESMISSPMPTRAEVS
CCEEEEEECCCCEEECCCCEEHHHHHHHEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDYTDAVMLSAESAAGSYPIEAVQAMARICIGAEKHPTGKTSSHRIGHSFTRCD
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCC
ESIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSVPIYAFSPHRGTQARAAMFRG
CHHEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCC
VYTVPFDPAALPPGQVSQAAVDELLKRGLVEQGDWVILTKGDSYHTIGGTNGMKILHVGD
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCEEEEECCC
PLV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]