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The map label for this gene is algU [H]

Identifier: 66047185

GI number: 66047185

Start: 4706018

End: 4706599

Strand: Reverse

Name: algU [H]

Synonym: Psyr_3958

Alternate gene names: 66047185

Gene position: 4706599-4706018 (Counterclockwise)

Preceding gene: 66047187

Following gene: 66047184

Centisome position: 77.24

GC content: 54.3

Gene sequence:

>582_bases
ATGCTAACCCAGGAAGAGGATCAGCAGCTTGTCGAGCGCGTACAGCGTGGCGATACGCGAGCATTTGATCTGTTGGTGCT
GAAGTATCAGCACAAAATTCTAGGGTTGATCGTGCGATTCGTGCACGACACCCATGAGGCTCAAGACGTTGCACAGGAAG
CCTTTATCAAGGCTTACCGTGCTCTCGGAAATTTTCGCGGTGACAGTGCTTTCTACACGTGGCTGTATCGCATCGCCATT
AACACGGCGAAGAACTATCTGGTCTCGCGAGGTCGGCGACCACCGGATAGCGATGTAAGGTCTGAAGACGCGGAGTTCTA
CGACGGCGACCACGGCCTCAAGGACATCGAGTCGCCGGAGCGTGCATTGTTGAGGGATGAGATCGAAGGCACCGTCCATC
GGACCATCCAGCTTCTCCCGGAAGATTTACGTACGGCACTAACTTTACGTGAATTCGATGGTCTGAGTTATGAAGACATT
GCGAGCGTCATGCAGTGTCCTGTTGGTACCGTGCGCTCTCGGATCTTCCGCGCTCGGGAAGCCATCGATAAAGCCCTGCA
GCCGTTGTTGCAGGAATCCTGA

Upstream 100 bases:

>100_bases
TGCTTGAAGGGGGAGAACTTTTGCGAAAAGCCCGAGTCTATGTTTGCGAGCCTGAACAATATCAGTTGCAACACTCCTTC
ATGCTCAACGAGGAGTGTTC

Downstream 100 bases:

>100_bases
GACAGCGGCGATAGCCCAAGAGAGGAACCCGCCATGAGTCGTGAAGCCCTGCAGGAATCGCTGTCCGCGGTACTGGATAA
CGAAGCGGACGAACTGGAAT

Product: RNA polymerase sigma factor AlgU

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MLTQEEDQQLVERVQRGDTRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYRALGNFRGDSAFYTWLYRIAI
NTAKNYLVSRGRRPPDSDVRSEDAEFYDGDHGLKDIESPERALLRDEIEGTVHRTIQLLPEDLRTALTLREFDGLSYEDI
ASVMQCPVGTVRSRIFRAREAIDKALQPLLQES

Sequences:

>Translated_193_residues
MLTQEEDQQLVERVQRGDTRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYRALGNFRGDSAFYTWLYRIAI
NTAKNYLVSRGRRPPDSDVRSEDAEFYDGDHGLKDIESPERALLRDEIEGTVHRTIQLLPEDLRTALTLREFDGLSYEDI
ASVMQCPVGTVRSRIFRAREAIDKALQPLLQES
>Mature_193_residues
MLTQEEDQQLVERVQRGDTRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYRALGNFRGDSAFYTWLYRIAI
NTAKNYLVSRGRRPPDSDVRSEDAEFYDGDHGLKDIESPERALLRDEIEGTVHRTIQLLPEDLRTALTLREFDGLSYEDI
ASVMQCPVGTVRSRIFRAREAIDKALQPLLQES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=63.8743455497382, Blast_Score=254, Evalue=2e-69,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22251; Mature: 22251

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQEEDQQLVERVQRGDTRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYR
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGNFRGDSAFYTWLYRIAINTAKNYLVSRGRRPPDSDVRSEDAEFYDGDHGLKDIESPE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCHHCCCHH
RALLRDEIEGTVHRTIQLLPEDLRTALTLREFDGLSYEDIASVMQCPVGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIDKALQPLLQES
HHHHHHHHHHCCC
>Mature Secondary Structure
MLTQEEDQQLVERVQRGDTRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYR
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGNFRGDSAFYTWLYRIAINTAKNYLVSRGRRPPDSDVRSEDAEFYDGDHGLKDIESPE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCHHCCCHH
RALLRDEIEGTVHRTIQLLPEDLRTALTLREFDGLSYEDIASVMQCPVGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIDKALQPLLQES
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]