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The map label for this gene is ddl [H]

Identifier: 66047146

GI number: 66047146

Start: 4661904

End: 4662995

Strand: Reverse

Name: ddl [H]

Synonym: Psyr_3919

Alternate gene names: 66047146

Gene position: 4662995-4661904 (Counterclockwise)

Preceding gene: 66047147

Following gene: 66047145

Centisome position: 76.52

GC content: 59.89

Gene sequence:

>1092_bases
TTGAAAAAGTCAGTCGCCATCGTTTTTGGTGGGCAATCCAGCGAGCATGAGGTTTCGTTGCAGTCGGCACGCAACGTGAT
CAATGCGATTGATCGTGAACGCTATGCACTGACCCTGATCGGCGTCGACAAGCTGGGCCGCTGGCTGCATTTCGACGAAG
CCGATTACCTGCTTAATGCCACTGATCCTGCACGGATCAAACTAAGCGCATCAGGCAAGCCATTGTCCTTGCTGCCGGGC
AGTATCGGCGGGCAGTTTGTCGACGTCGAGTCAGGCCAGCCACTGGCAGCCATCGATGTGGTATTCCCGCTGATCCACGG
CGCTTTTGGTGAAGACGGCGCGTTGCAGGGTCTGCTGCGCATGCTGTCCGTGCCTTTTGTCGGCGCCGATGTGCTGTCCT
CTGCGGCCTGCATGGACAAGGACGTGACCAAGCGTCTGCTGCGCGATGCGGGCATTGCCGTCGCGCCGTTCAGGGTCCTG
TCGCGTGGAGAGAGCCTTTCTTTCACGGAGGCTGCCGACCAGTTGGGGCTGCCGATGTTCGTCAAGCCGGCCAACCAGGG
TTCATCGGTGGGCGTGAGCAAGGTGTCTGACGAGGCCGGTTTCGCGGCAGCACTGGCGCTGGGTTTCGAGTTCGATCACA
AGCTGCTGATCGAGCAGGGCATCGTTGGCCGGGAAGTGGAGTGCGCGGTATTGGGTAACTTCGATGCGCAGGTCAGTGTC
TGTGGCGAAGTGATTGCCAACGATGAATTCTATGCCTACGACACCAAGTACCTGAATGGCGATCAGGCACGCATCGCCAT
TCCCGCCGAGCTGCCCGCCGACCTGAGTGATCAGGTGCGTGACGTCGCGTTGCAGGCGTACAAGGTGCTGGGCTGCGCAG
GGCTCTCGCGGGTCGATTTCTTCGTCACCGAAGGGCGCGACATCATCATCAACGAGGTCAACACGCTGCCGGGCTTCACC
TCGATCAGCATGTACCCCAAGCTCTGGCAGGCCAGCGGTCTGTCGTATGCCGAACTGATCCATCGCCTGATCGTTCTGGC
GCTGGAACGGGCTGATCAGGCGCGGCTGCTGAAAACGGAGATCTTTCCATGA

Upstream 100 bases:

>100_bases
GATTTTTTTGTCTGAATTTCAGGCCAACGGCCAATCATCTACTGGACTAACAGCCGACATTGCTTTTATCTTCGCGGCCT
GAAAAACAGGAGTAACCGCT

Downstream 100 bases:

>100_bases
AGATCATCCGCATCGCGGCGGCGCTGCTGATCGGTGCAGATGGCCGGACCCTGCTGGTGCGCAAGCGCGGTACCCAGGCC
TTCATGCAGCCGGGCGGCAA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKKSVAIVFGGQSSEHEVSLQSARNVINAIDRERYALTLIGVDKLGRWLHFDEADYLLNATDPARIKLSASGKPLSLLPG
SIGGQFVDVESGQPLAAIDVVFPLIHGAFGEDGALQGLLRMLSVPFVGADVLSSAACMDKDVTKRLLRDAGIAVAPFRVL
SRGESLSFTEAADQLGLPMFVKPANQGSSVGVSKVSDEAGFAAALALGFEFDHKLLIEQGIVGREVECAVLGNFDAQVSV
CGEVIANDEFYAYDTKYLNGDQARIAIPAELPADLSDQVRDVALQAYKVLGCAGLSRVDFFVTEGRDIIINEVNTLPGFT
SISMYPKLWQASGLSYAELIHRLIVLALERADQARLLKTEIFP

Sequences:

>Translated_363_residues
MKKSVAIVFGGQSSEHEVSLQSARNVINAIDRERYALTLIGVDKLGRWLHFDEADYLLNATDPARIKLSASGKPLSLLPG
SIGGQFVDVESGQPLAAIDVVFPLIHGAFGEDGALQGLLRMLSVPFVGADVLSSAACMDKDVTKRLLRDAGIAVAPFRVL
SRGESLSFTEAADQLGLPMFVKPANQGSSVGVSKVSDEAGFAAALALGFEFDHKLLIEQGIVGREVECAVLGNFDAQVSV
CGEVIANDEFYAYDTKYLNGDQARIAIPAELPADLSDQVRDVALQAYKVLGCAGLSRVDFFVTEGRDIIINEVNTLPGFT
SISMYPKLWQASGLSYAELIHRLIVLALERADQARLLKTEIFP
>Mature_363_residues
MKKSVAIVFGGQSSEHEVSLQSARNVINAIDRERYALTLIGVDKLGRWLHFDEADYLLNATDPARIKLSASGKPLSLLPG
SIGGQFVDVESGQPLAAIDVVFPLIHGAFGEDGALQGLLRMLSVPFVGADVLSSAACMDKDVTKRLLRDAGIAVAPFRVL
SRGESLSFTEAADQLGLPMFVKPANQGSSVGVSKVSDEAGFAAALALGFEFDHKLLIEQGIVGREVECAVLGNFDAQVSV
CGEVIANDEFYAYDTKYLNGDQARIAIPAELPADLSDQVRDVALQAYKVLGCAGLSRVDFFVTEGRDIIINEVNTLPGFT
SISMYPKLWQASGLSYAELIHRLIVLALERADQARLLKTEIFP

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=362, Percent_Identity=58.2872928176796, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1786280, Length=352, Percent_Identity=33.2386363636364, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39017; Mature: 39017

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVAIVFGGQSSEHEVSLQSARNVINAIDRERYALTLIGVDKLGRWLHFDEADYLLNA
CCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEEEEHHHHCCEEEECCCCEEEEC
TDPARIKLSASGKPLSLLPGSIGGQFVDVESGQPLAAIDVVFPLIHGAFGEDGALQGLLR
CCCCEEEEECCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
MLSVPFVGADVLSSAACMDKDVTKRLLRDAGIAVAPFRVLSRGESLSFTEAADQLGLPMF
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHCCCCCCHHHHHHHCCCCEE
VKPANQGSSVGVSKVSDEAGFAAALALGFEFDHKLLIEQGIVGREVECAVLGNFDAQVSV
EEECCCCCCCCCHHCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEECCCCHHHH
CGEVIANDEFYAYDTKYLNGDQARIAIPAELPADLSDQVRDVALQAYKVLGCAGLSRVDF
HHHHHCCCCEEEEECEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
FVTEGRDIIINEVNTLPGFTSISMYPKLWQASGLSYAELIHRLIVLALERADQARLLKTE
EEECCCEEEEEECCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHC
IFP
CCC
>Mature Secondary Structure
MKKSVAIVFGGQSSEHEVSLQSARNVINAIDRERYALTLIGVDKLGRWLHFDEADYLLNA
CCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEEEEHHHHCCEEEECCCCEEEEC
TDPARIKLSASGKPLSLLPGSIGGQFVDVESGQPLAAIDVVFPLIHGAFGEDGALQGLLR
CCCCEEEEECCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
MLSVPFVGADVLSSAACMDKDVTKRLLRDAGIAVAPFRVLSRGESLSFTEAADQLGLPMF
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHCCCCCCHHHHHHHCCCCEE
VKPANQGSSVGVSKVSDEAGFAAALALGFEFDHKLLIEQGIVGREVECAVLGNFDAQVSV
EEECCCCCCCCCHHCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEECCCCHHHH
CGEVIANDEFYAYDTKYLNGDQARIAIPAELPADLSDQVRDVALQAYKVLGCAGLSRVDF
HHHHHCCCCEEEEECEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
FVTEGRDIIINEVNTLPGFTSISMYPKLWQASGLSYAELIHRLIVLALERADQARLLKTE
EEECCCEEEEEECCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHC
IFP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]