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The map label for this gene is nasC [H]

Identifier: 66047126

GI number: 66047126

Start: 4639221

End: 4641329

Strand: Reverse

Name: nasC [H]

Synonym: Psyr_3899

Alternate gene names: 66047126

Gene position: 4641329-4639221 (Counterclockwise)

Preceding gene: 66047127

Following gene: 66047125

Centisome position: 76.17

GC content: 61.69

Gene sequence:

>2109_bases
ATGACCCGGACTGAACACTACCGAGCCTGCCACCTGTGCGAGGCCATTTGCGGTCTTGTGATCGAGACCGTCGTGGAGGC
GGATTCTGCGCCGCGCATTGCCTCGATCAAGGGCGACCCGCTGGACACGTTCAGCCGCGGTCATATCTGCCCCAAGGCGA
TTGCGTTGCAGGACATCCAGAATGACCCTGATCGTTTGCGCCAGCCGATGCTGCGCAGCGGTGATCAGTGGCAGCCGATT
GCCTGGCAGCAGGCGTTCGATCTGGTGGCGGAGCGGCTTTACGCCATTCAGCAGCGCCATGGGCAGAACGCGGTAGCTGT
CTATCAGGGCAACCCCAGCGTGCATAACTACGGGTTGATGACCCACAGCAATTACTTTCTCGGCCTGCTCAAGACCCGCA
ATCGTTTTTCGGCGACGTCGGTCGATCAGTTGCCGCACCACCTCAGCAGTTTCCTGATGTATGGCCACGGCATGCTGTTG
CCGATTCCGGATATCGATCACACCGACTTCATGCTGATTCTCGGCGGCAATCCGTTGGCGTCCAATGGCAGCATCATGAC
GGTTCCGGATGTTGAAAAGCGTCTGAAAGCGATCCAGCAGCGCGGCGGCAAACTGGTGGTCATCGACCCGCGCCGCAGTG
AGACGGCTGCCATCGCTGACCAGCATCTGTTCGTACGTCCGGGCGGTGACGCAGCGTTGCTGTTCGGGCTGCTCAATACG
CTGTTCGAAGAGGGGCTGACCCGCGAGAGCCATTTGCCTGTCGACGGCCTGGAGCAGGTTCGCCAGGCCATTGCCGGCTT
CACTGCAGAGGCCATGAGCCCGCGCTGCGGTATCGCCGCCGAGCAGATCCGGCAACTGGCACGGGATTTCGCGGCCGCTG
ACAAGGCGGTGTGTTATGGGCGAATGGGCGTGTCGACTCAGACCTTTGGCACGCTGTGTCATTGGCTGGCACAACTGATC
AATCTGGTGACGGGCAACCTGGACCGTGTGGGTGGCGCACTCTGTACGGAGCCCGCCGTCGACCTCGTCAGCTCGACATC
CGGCGGTCATTTCAATCGGTGGCAAAGCAGGGTGTCCGGCCTGCCAGAGTATGGCGGCGAGTTGCCGGTTTCGGCGCTGG
CCGAGGAAATGCTTGTGGACGGGGAAGGGCAGGTTCGCGCGTTGGTGACCGTTGCGGGTAACCCGGTTCTGTCGACGCCC
AACGGCCGTCAGCTGGAGCAGGCGCTCGAAGGGCTTGAGTTCATGCTCAGCATCGATCTTTACATCAACGAGACCACTCG
CCACGCCGACTTGATCCTGCCGTCCACCTCGGCACTGGAAAATGATCACTACGACACCACCTTCAACACGCTCGCAGTGC
GCAACGTCACGCGCTTCAATCGGGCCATTTTCGACAAGCCCGAAGGTGCCCTGCACGACTGGGAGATCTTCGTCGGGCTG
GCCAGCGCCTTTGCTGCCAAGGCCGAGCGAGCGCTGAAACCCACGTTGCCGCCTGCGCAGATGATCGATCGCGGGCTGCG
TGCAGGCCTCTATGGCGATGCATCGTCCCATAAGCTGTCACTGGAAACCTTGGACAGTCATCCGCACGGCCTCGATCTGG
GGGCGTTGAAGGCCAATCTTGCACAGCGCCTGAAGACCGCCAATGGCCGCATTCAGGCCGCGCCTGAGGTGATCATGGCC
GACCTGGCGCGCTTTGCAGCCGATCCTTCTCCTCAGGCAAGCGAGTTGCTGCTGATTGGCCGCAGGCATGTGCGCAGTAA
CAATTCATGGATGCACAACTACCATCGGCTGGTGAAGGGCAAACCCAGGCATCAACTGTTGATGCATCCTGACGATCTCG
CGTGCCGTGGTCTGAGTGACGGCCAGCAGGTCCGGGTCAGCTCCCGGGTCGGGATGATCGAGGTTCAGGTGCTGGGCAGT
CTGGACATGATGCGCGGGGTGGTCAGCCTGCCGCACGGTTGGGGGCATTCGCGTTCAGGGGTAAAAATGGCGATCGCGCA
GAGCCTGCCCGGCGTCAGTGCCAATGACCTGACCGACGAACGGCAACTCGACGTGCTGTCCGGCAACGCGGCGTTGAACG
GCGTACCGGTGCAGGTCGTGGCGTGTTAA

Upstream 100 bases:

>100_bases
TCGATTCACGGGCCGAACATCAGTGGCGCGGGGCATGAGCAGACAGCATAGTGAGTGTTCGGCAATCAACCGGATTGCCA
TGACACGATCTGAGGCTGTG

Downstream 100 bases:

>100_bases
GCCTCAAGACAGAGCGTCATGCTCGGGATTTCGTTACAATGTGTCACCGTGCCGACAACTGAGTCGGATATTTCAGCCGA
GGTGCTCCATGGATATCATC

Product: molybdopterin oxidoreductase:molydopterin dinucleotide-binding region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MTRTEHYRACHLCEAICGLVIETVVEADSAPRIASIKGDPLDTFSRGHICPKAIALQDIQNDPDRLRQPMLRSGDQWQPI
AWQQAFDLVAERLYAIQQRHGQNAVAVYQGNPSVHNYGLMTHSNYFLGLLKTRNRFSATSVDQLPHHLSSFLMYGHGMLL
PIPDIDHTDFMLILGGNPLASNGSIMTVPDVEKRLKAIQQRGGKLVVIDPRRSETAAIADQHLFVRPGGDAALLFGLLNT
LFEEGLTRESHLPVDGLEQVRQAIAGFTAEAMSPRCGIAAEQIRQLARDFAAADKAVCYGRMGVSTQTFGTLCHWLAQLI
NLVTGNLDRVGGALCTEPAVDLVSSTSGGHFNRWQSRVSGLPEYGGELPVSALAEEMLVDGEGQVRALVTVAGNPVLSTP
NGRQLEQALEGLEFMLSIDLYINETTRHADLILPSTSALENDHYDTTFNTLAVRNVTRFNRAIFDKPEGALHDWEIFVGL
ASAFAAKAERALKPTLPPAQMIDRGLRAGLYGDASSHKLSLETLDSHPHGLDLGALKANLAQRLKTANGRIQAAPEVIMA
DLARFAADPSPQASELLLIGRRHVRSNNSWMHNYHRLVKGKPRHQLLMHPDDLACRGLSDGQQVRVSSRVGMIEVQVLGS
LDMMRGVVSLPHGWGHSRSGVKMAIAQSLPGVSANDLTDERQLDVLSGNAALNGVPVQVVAC

Sequences:

>Translated_702_residues
MTRTEHYRACHLCEAICGLVIETVVEADSAPRIASIKGDPLDTFSRGHICPKAIALQDIQNDPDRLRQPMLRSGDQWQPI
AWQQAFDLVAERLYAIQQRHGQNAVAVYQGNPSVHNYGLMTHSNYFLGLLKTRNRFSATSVDQLPHHLSSFLMYGHGMLL
PIPDIDHTDFMLILGGNPLASNGSIMTVPDVEKRLKAIQQRGGKLVVIDPRRSETAAIADQHLFVRPGGDAALLFGLLNT
LFEEGLTRESHLPVDGLEQVRQAIAGFTAEAMSPRCGIAAEQIRQLARDFAAADKAVCYGRMGVSTQTFGTLCHWLAQLI
NLVTGNLDRVGGALCTEPAVDLVSSTSGGHFNRWQSRVSGLPEYGGELPVSALAEEMLVDGEGQVRALVTVAGNPVLSTP
NGRQLEQALEGLEFMLSIDLYINETTRHADLILPSTSALENDHYDTTFNTLAVRNVTRFNRAIFDKPEGALHDWEIFVGL
ASAFAAKAERALKPTLPPAQMIDRGLRAGLYGDASSHKLSLETLDSHPHGLDLGALKANLAQRLKTANGRIQAAPEVIMA
DLARFAADPSPQASELLLIGRRHVRSNNSWMHNYHRLVKGKPRHQLLMHPDDLACRGLSDGQQVRVSSRVGMIEVQVLGS
LDMMRGVVSLPHGWGHSRSGVKMAIAQSLPGVSANDLTDERQLDVLSGNAALNGVPVQVVAC
>Mature_701_residues
TRTEHYRACHLCEAICGLVIETVVEADSAPRIASIKGDPLDTFSRGHICPKAIALQDIQNDPDRLRQPMLRSGDQWQPIA
WQQAFDLVAERLYAIQQRHGQNAVAVYQGNPSVHNYGLMTHSNYFLGLLKTRNRFSATSVDQLPHHLSSFLMYGHGMLLP
IPDIDHTDFMLILGGNPLASNGSIMTVPDVEKRLKAIQQRGGKLVVIDPRRSETAAIADQHLFVRPGGDAALLFGLLNTL
FEEGLTRESHLPVDGLEQVRQAIAGFTAEAMSPRCGIAAEQIRQLARDFAAADKAVCYGRMGVSTQTFGTLCHWLAQLIN
LVTGNLDRVGGALCTEPAVDLVSSTSGGHFNRWQSRVSGLPEYGGELPVSALAEEMLVDGEGQVRALVTVAGNPVLSTPN
GRQLEQALEGLEFMLSIDLYINETTRHADLILPSTSALENDHYDTTFNTLAVRNVTRFNRAIFDKPEGALHDWEIFVGLA
SAFAAKAERALKPTLPPAQMIDRGLRAGLYGDASSHKLSLETLDSHPHGLDLGALKANLAQRLKTANGRIQAAPEVIMAD
LARFAADPSPQASELLLIGRRHVRSNNSWMHNYHRLVKGKPRHQLLMHPDDLACRGLSDGQQVRVSSRVGMIEVQVLGSL
DMMRGVVSLPHGWGHSRSGVKMAIAQSLPGVSANDLTDERQLDVLSGNAALNGVPVQVVAC

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=506, Percent_Identity=27.6679841897233, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1787870, Length=733, Percent_Identity=22.7830832196453, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI3868720, Length=269, Percent_Identity=29.7397769516729, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI3868719, Length=252, Percent_Identity=28.1746031746032, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI171474008, Length=359, Percent_Identity=26.1838440111421, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787778, Length=480, Percent_Identity=25.625, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081994, Length=725, Percent_Identity=20.551724137931, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 76464; Mature: 76333

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTEHYRACHLCEAICGLVIETVVEADSAPRIASIKGDPLDTFSRGHICPKAIALQDIQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHC
NDPDRLRQPMLRSGDQWQPIAWQQAFDLVAERLYAIQQRHGQNAVAVYQGNPSVHNYGLM
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECEEE
THSNYFLGLLKTRNRFSATSVDQLPHHLSSFLMYGHGMLLPIPDIDHTDFMLILGGNPLA
ECCCHHEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCC
SNGSIMTVPDVEKRLKAIQQRGGKLVVIDPRRSETAAIADQHLFVRPGGDAALLFGLLNT
CCCCEEECCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCEEEEECCCCHHHHHHHHHH
LFEEGLTRESHLPVDGLEQVRQAIAGFTAEAMSPRCGIAAEQIRQLARDFAAADKAVCYG
HHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEC
RMGVSTQTFGTLCHWLAQLINLVTGNLDRVGGALCTEPAVDLVSSTSGGHFNRWQSRVSG
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHCCHHHHHHHCCCCCCHHHHHHHHCC
LPEYGGELPVSALAEEMLVDGEGQVRALVTVAGNPVLSTPNGRQLEQALEGLEFMLSIDL
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHEEEEEEE
YINETTRHADLILPSTSALENDHYDTTFNTLAVRNVTRFNRAIFDKPEGALHDWEIFVGL
EEECCCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ASAFAAKAERALKPTLPPAQMIDRGLRAGLYGDASSHKLSLETLDSHPHGLDLGALKANL
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHH
AQRLKTANGRIQAAPEVIMADLARFAADPSPQASELLLIGRRHVRSNNSWMHNYHRLVKG
HHHHHHCCCCEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCC
KPRHQLLMHPDDLACRGLSDGQQVRVSSRVGMIEVQVLGSLDMMRGVVSLPHGWGHSRSG
CCCCCEEECCCCHHHCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCC
VKMAIAQSLPGVSANDLTDERQLDVLSGNAALNGVPVQVVAC
CEEHHHHHCCCCCCCCCCCCHHEEEEECCCEECCCCEEEEEC
>Mature Secondary Structure 
TRTEHYRACHLCEAICGLVIETVVEADSAPRIASIKGDPLDTFSRGHICPKAIALQDIQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHC
NDPDRLRQPMLRSGDQWQPIAWQQAFDLVAERLYAIQQRHGQNAVAVYQGNPSVHNYGLM
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECEEE
THSNYFLGLLKTRNRFSATSVDQLPHHLSSFLMYGHGMLLPIPDIDHTDFMLILGGNPLA
ECCCHHEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCC
SNGSIMTVPDVEKRLKAIQQRGGKLVVIDPRRSETAAIADQHLFVRPGGDAALLFGLLNT
CCCCEEECCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCEEEEECCCCHHHHHHHHHH
LFEEGLTRESHLPVDGLEQVRQAIAGFTAEAMSPRCGIAAEQIRQLARDFAAADKAVCYG
HHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEC
RMGVSTQTFGTLCHWLAQLINLVTGNLDRVGGALCTEPAVDLVSSTSGGHFNRWQSRVSG
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHCCHHHHHHHCCCCCCHHHHHHHHCC
LPEYGGELPVSALAEEMLVDGEGQVRALVTVAGNPVLSTPNGRQLEQALEGLEFMLSIDL
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHEEEEEEE
YINETTRHADLILPSTSALENDHYDTTFNTLAVRNVTRFNRAIFDKPEGALHDWEIFVGL
EEECCCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ASAFAAKAERALKPTLPPAQMIDRGLRAGLYGDASSHKLSLETLDSHPHGLDLGALKANL
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHH
AQRLKTANGRIQAAPEVIMADLARFAADPSPQASELLLIGRRHVRSNNSWMHNYHRLVKG
HHHHHHCCCCEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCC
KPRHQLLMHPDDLACRGLSDGQQVRVSSRVGMIEVQVLGSLDMMRGVVSLPHGWGHSRSG
CCCCCEEECCCCHHHCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCC
VKMAIAQSLPGVSANDLTDERQLDVLSGNAALNGVPVQVVAC
CEEHHHHHCCCCCCCCCCCCHHEEEEECCCEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]