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The map label for this gene is nth [H]

Identifier: 66047115

GI number: 66047115

Start: 4629580

End: 4630218

Strand: Direct

Name: nth [H]

Synonym: Psyr_3888

Alternate gene names: 66047115

Gene position: 4629580-4630218 (Clockwise)

Preceding gene: 66047114

Following gene: 66047116

Centisome position: 75.97

GC content: 55.56

Gene sequence:

>639_bases
ATGAATGCCGCAAAACGCCAGGAAATCTTTCGCAGGCTGCATGAGGACAATCCGGACCCAAAAACCGAATTGGCCTACAC
CACGCCTTTCGAACTGTTGATAGCCGTCATCCTTTCCGCCCAGGCCACCGACGTAAGCGTCAATAAGGCGACTGCCAGGC
TCTACCCGATAGCCAACACCCCACAGGCCATCTATGAACTGGGTGTAGAGGGGCTTTCCGAGTACATCAAGACCATCGGC
CTCTACAACAGCAAAGCCAAAAACGTGATCGAAACCTGCCGCATGCTGGTGGAGCTGCACAACGGTGAAGTACCGCAAAC
CCGAGAAGCACTCGAGGCGTTGCCGGGCGTCGGGCGCAAAACCGCTAACGTGGTGCTCAACACCGCTTTCCGCCAGATCG
CGATGGCGGTGGACACGCATATCTTCCGCGTCAGCAACCGCACAGGCATCGCGCCAGGGAAAAATGTGGTGGAAGTGGAA
AGGCAACTGATGAAATTTGTGCCGAAAAACTACCTGCTCGACGCTCATCACTGGCTGATTCTGCACGGGCGCTATGTTTG
CCAGGCCCGTAAACCGCGATGTGGCAGTTGCCGTATAGAAGACCTGTGCGATTACAAGGCAAAAACGTCCGACGATTGA

Upstream 100 bases:

>100_bases
GGCTTGTGGAATTGCAGCGCGAATACGAAGCTGCCGAGCAGGCACTTGCAGCGCTTCTACCGCTCCCCGCACAGGCTCAT
TCCGAGGAAAAGCTCCGCCC

Downstream 100 bases:

>100_bases
GCCAGACAGCTTATTGCTGTCCTGGCGATTGAAAAAATCTTTTTTACCGACCTGTGGATTGTCGATATAAGAAGCGCCAG
AGAGCCCAAGCCCTGGAGTC

Product: endonuclease III/Nth

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNAAKRQEIFRRLHEDNPDPKTELAYTTPFELLIAVILSAQATDVSVNKATARLYPIANTPQAIYELGVEGLSEYIKTIG
LYNSKAKNVIETCRMLVELHNGEVPQTREALEALPGVGRKTANVVLNTAFRQIAMAVDTHIFRVSNRTGIAPGKNVVEVE
RQLMKFVPKNYLLDAHHWLILHGRYVCQARKPRCGSCRIEDLCDYKAKTSDD

Sequences:

>Translated_212_residues
MNAAKRQEIFRRLHEDNPDPKTELAYTTPFELLIAVILSAQATDVSVNKATARLYPIANTPQAIYELGVEGLSEYIKTIG
LYNSKAKNVIETCRMLVELHNGEVPQTREALEALPGVGRKTANVVLNTAFRQIAMAVDTHIFRVSNRTGIAPGKNVVEVE
RQLMKFVPKNYLLDAHHWLILHGRYVCQARKPRCGSCRIEDLCDYKAKTSDD
>Mature_212_residues
MNAAKRQEIFRRLHEDNPDPKTELAYTTPFELLIAVILSAQATDVSVNKATARLYPIANTPQAIYELGVEGLSEYIKTIG
LYNSKAKNVIETCRMLVELHNGEVPQTREALEALPGVGRKTANVVLNTAFRQIAMAVDTHIFRVSNRTGIAPGKNVVEVE
RQLMKFVPKNYLLDAHHWLILHGRYVCQARKPRCGSCRIEDLCDYKAKTSDD

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=28.8135593220339, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=73.5576923076923, Blast_Score=323, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=32.4175824175824, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=25.531914893617, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=27.0114942528736, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550361, Length=182, Percent_Identity=31.3186813186813, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23836; Mature: 23836

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAKRQEIFRRLHEDNPDPKTELAYTTPFELLIAVILSAQATDVSVNKATARLYPIANT
CCHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCC
PQAIYELGVEGLSEYIKTIGLYNSKAKNVIETCRMLVELHNGEVPQTREALEALPGVGRK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
TANVVLNTAFRQIAMAVDTHIFRVSNRTGIAPGKNVVEVERQLMKFVPKNYLLDAHHWLI
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCHHHEECCCCEEE
LHGRYVCQARKPRCGSCRIEDLCDYKAKTSDD
EECHHHCCCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MNAAKRQEIFRRLHEDNPDPKTELAYTTPFELLIAVILSAQATDVSVNKATARLYPIANT
CCHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCC
PQAIYELGVEGLSEYIKTIGLYNSKAKNVIETCRMLVELHNGEVPQTREALEALPGVGRK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
TANVVLNTAFRQIAMAVDTHIFRVSNRTGIAPGKNVVEVERQLMKFVPKNYLLDAHHWLI
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCHHHEECCCCEEE
LHGRYVCQARKPRCGSCRIEDLCDYKAKTSDD
EECHHHCCCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]