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The map label for this gene is braC [H]

Identifier: 66047072

GI number: 66047072

Start: 4583721

End: 4584845

Strand: Direct

Name: braC [H]

Synonym: Psyr_3845

Alternate gene names: 66047072

Gene position: 4583721-4584845 (Clockwise)

Preceding gene: 66047070

Following gene: 66047073

Centisome position: 75.22

GC content: 59.2

Gene sequence:

>1125_bases
ATGACTAAGGGTACCAATAAGCTTTCAAGGCTGTTTGCCGCACTGGTTATGGCGGGGGTTGCCAGCCATTCGTTCGCAGC
TGACACCATCAAGATCGGCATCGCCGGCCCCAAGACCGGCCCTGTCGCCGAATACGGCACCATGCAGTTCAATGGTGCCA
GGATGGCCATCGAGCAGATCAACGCCAAAGGCGGCGTAGATGGCAAAAAGCTTGAAGCCGTCGAATATGATGACGCCTGC
GATCCGAAACAGGCCGTTGCGGTCGCCAACAAGGTGGTCAACGACGGCGTGAAATACGTGGTCGGCCACCTCTGCTCTAG
CTCCGCCCAGCCAGCCTCCGACGTTTACGAAGACGAAGGCATCATCATGGTGACCCCGGCTGCCACCAGCCCGGAAATCA
CCGCGCGCGGCTACAAACTGGTGTTCCGCACCATCGGTCTCGACAGCGCCCAGGGTCCGGCCGCTGGCAACTACATTGCC
GACGTCGCCAAGCCGAAAATCGTTGCAGTGATCCACGACAAGCAGCAGTACGGCGAAGGTATCGCGACAGCCGTCAAGCA
AACGCTGGAGAAAAAAGGCGTCAAGGTTGCACTGTTCGAAGGCATCAACGCTGGCGACAAGGACTTCTCCTCGCTGATTC
AAAAACTCAAGCAGGCCAACGTCGACTTCGTCTACTACGGCGGCTACCACCCTGAGCTGGGCCAGATCCTGCGCCAGTCC
AAGGAAAAAGGCCTGAACGCCAAGTTCATGGGCCCTGAAGGCGTCGGCAACGAGTCGATCTCGCAAATTGCTGGCGAAGC
TTCCGAAGGCCTGCTGGTCACCCTGCCAAAATCCTTCGACCAGGACCCGGCCAACCAGGCGCTGACCGAGGCCTTCAAGG
CCAAGAGTCAGGACCCGAGCGGCCCGTTCGTGTACCCGTCCTACTCCGCCGTACAGGTCATTGCTGACGGCATCGCTGCC
GCCAAGTCCGAAGACACCACCAAGGTGGCTGCTGCCATCCACGCCGGTACCTTCAAGACGCCAATGGGCGAGCTGAGCTA
CGACGAAAAAGGTGACCTGAAGAACTTCCAGTTCGTGGTCTACGAGTGGCACTTCGGCAAGCCCAAGACCGAAGCCGCCA
AATAA

Upstream 100 bases:

>100_bases
GTTCCGGTACAGTTGGCGGTGCGGGACAACCGGTGATCGAATCGGTCCTGGAGCCTTGGCCATCAGGATTATAAAAACTA
CAACGCAAGAGTGGAGCATC

Downstream 100 bases:

>100_bases
GCTGCTTGCGTTAACCCTCAAAGCCCACTGCCAGCGCAGTGGGCTTTGTTTTACGAATCCAGGCCCGTACGCAGGTTTCG
GCAACCCGAACTGTATGCAC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTKGTNKLSRLFAALVMAGVASHSFAADTIKIGIAGPKTGPVAEYGTMQFNGARMAIEQINAKGGVDGKKLEAVEYDDAC
DPKQAVAVANKVVNDGVKYVVGHLCSSSAQPASDVYEDEGIIMVTPAATSPEITARGYKLVFRTIGLDSAQGPAAGNYIA
DVAKPKIVAVIHDKQQYGEGIATAVKQTLEKKGVKVALFEGINAGDKDFSSLIQKLKQANVDFVYYGGYHPELGQILRQS
KEKGLNAKFMGPEGVGNESISQIAGEASEGLLVTLPKSFDQDPANQALTEAFKAKSQDPSGPFVYPSYSAVQVIADGIAA
AKSEDTTKVAAAIHAGTFKTPMGELSYDEKGDLKNFQFVVYEWHFGKPKTEAAK

Sequences:

>Translated_374_residues
MTKGTNKLSRLFAALVMAGVASHSFAADTIKIGIAGPKTGPVAEYGTMQFNGARMAIEQINAKGGVDGKKLEAVEYDDAC
DPKQAVAVANKVVNDGVKYVVGHLCSSSAQPASDVYEDEGIIMVTPAATSPEITARGYKLVFRTIGLDSAQGPAAGNYIA
DVAKPKIVAVIHDKQQYGEGIATAVKQTLEKKGVKVALFEGINAGDKDFSSLIQKLKQANVDFVYYGGYHPELGQILRQS
KEKGLNAKFMGPEGVGNESISQIAGEASEGLLVTLPKSFDQDPANQALTEAFKAKSQDPSGPFVYPSYSAVQVIADGIAA
AKSEDTTKVAAAIHAGTFKTPMGELSYDEKGDLKNFQFVVYEWHFGKPKTEAAK
>Mature_373_residues
TKGTNKLSRLFAALVMAGVASHSFAADTIKIGIAGPKTGPVAEYGTMQFNGARMAIEQINAKGGVDGKKLEAVEYDDACD
PKQAVAVANKVVNDGVKYVVGHLCSSSAQPASDVYEDEGIIMVTPAATSPEITARGYKLVFRTIGLDSAQGPAAGNYIAD
VAKPKIVAVIHDKQQYGEGIATAVKQTLEKKGVKVALFEGINAGDKDFSSLIQKLKQANVDFVYYGGYHPELGQILRQSK
EKGLNAKFMGPEGVGNESISQIAGEASEGLLVTLPKSFDQDPANQALTEAFKAKSQDPSGPFVYPSYSAVQVIADGIAAA
KSEDTTKVAAAIHAGTFKTPMGELSYDEKGDLKNFQFVVYEWHFGKPKTEAAK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=356, Percent_Identity=57.0224719101124, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI48994941, Length=361, Percent_Identity=57.8947368421053, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39608; Mature: 39477

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGTNKLSRLFAALVMAGVASHSFAADTIKIGIAGPKTGPVAEYGTMQFNGARMAIEQI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHH
NAKGGVDGKKLEAVEYDDACDPKQAVAVANKVVNDGVKYVVGHLCSSSAQPASDVYEDEG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
IIMVTPAATSPEITARGYKLVFRTIGLDSAQGPAAGNYIADVAKPKIVAVIHDKQQYGEG
EEEEECCCCCCCCHHCHHEEEEEECCCCCCCCCCCCCHHHHCCCCEEEEEEECHHHHCCH
IATAVKQTLEKKGVKVALFEGINAGDKDFSSLIQKLKQANVDFVYYGGYHPELGQILRQS
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
KEKGLNAKFMGPEGVGNESISQIAGEASEGLLVTLPKSFDQDPANQALTEAFKAKSQDPS
HHCCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
GPFVYPSYSAVQVIADGIAAAKSEDTTKVAAAIHAGTFKTPMGELSYDEKGDLKNFQFVV
CCEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
YEWHFGKPKTEAAK
EEEECCCCCCCCCC
>Mature Secondary Structure 
TKGTNKLSRLFAALVMAGVASHSFAADTIKIGIAGPKTGPVAEYGTMQFNGARMAIEQI
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHH
NAKGGVDGKKLEAVEYDDACDPKQAVAVANKVVNDGVKYVVGHLCSSSAQPASDVYEDEG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
IIMVTPAATSPEITARGYKLVFRTIGLDSAQGPAAGNYIADVAKPKIVAVIHDKQQYGEG
EEEEECCCCCCCCHHCHHEEEEEECCCCCCCCCCCCCHHHHCCCCEEEEEEECHHHHCCH
IATAVKQTLEKKGVKVALFEGINAGDKDFSSLIQKLKQANVDFVYYGGYHPELGQILRQS
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
KEKGLNAKFMGPEGVGNESISQIAGEASEGLLVTLPKSFDQDPANQALTEAFKAKSQDPS
HHCCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
GPFVYPSYSAVQVIADGIAAAKSEDTTKVAAAIHAGTFKTPMGELSYDEKGDLKNFQFVV
CCEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
YEWHFGKPKTEAAK
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]