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The map label for this gene is proP [H]

Identifier: 66046996

GI number: 66046996

Start: 4485561

End: 4486877

Strand: Reverse

Name: proP [H]

Synonym: Psyr_3768

Alternate gene names: 66046996

Gene position: 4486877-4485561 (Counterclockwise)

Preceding gene: 66046997

Following gene: 66046995

Centisome position: 73.63

GC content: 61.5

Gene sequence:

>1317_bases
ATGACGGACATCGTACGTTCCCACTCCCAGCCCTTCAGCAAAGCCGACTACAAGACCCTGAGCCTTGCGGCGCTGGGTGG
GGCACTGGAAATCTACGACTTCATCATTTTCGTGTTCTTTGCGCTGACCCTCAGCCAGTTGTTCTTTCCCCCGGACATGC
CTGAATGGCTGCGTCTGCTGCAAAGTTTCGGGATCTTCGTGACCGGCTATCTGGCACGCCCGCTGGGCGGGATACTCATG
GCGCACTTCGCCGACCGGCTGGGCCGCAAGCGGGTGTTCAGCCTGAGCATTCTGATGATGGCGCTGCCTTGTCTGCTGAT
CGGCGTGATGCCCACCTACGCACAGATCGGTTACTGGGCGCCGCTGGTGCTGCTGGCGCTGCGCATCCTGCAAGGAGCCG
CCGTGGGCGGTGAAGTGCCAAGTGCCTGGGTATTCGTGGCAGAACACGCGCCCAAGGGGCATCGCGGCTATGCACTGGGC
GTACTGCAAGCCGGCCTGACGTTCGGATACCTGCTTGGCGCACTGACCGCGACATGGCTGGCGCGAGTCTTCAGCCCTGA
CCAGATCCTTGAGTGGGCATGGCGTATTCCGTTCCTGCTCGGTGGCGTATTCGGCGTGGTGGGGGTCTGGCTACGCCGCT
GGCTGAACGAAACCCCTGTGTTCATCGCCATGCATGCCCAGCGTGAACATCTGCCGGCGCTGCCACTTGGCCAGGTTCTG
CGTGATCACCGCCAATCCTTGCTGCCTGCTGCGTTGCTGACCTTTGTGCTGACCTCCGCCGTGGTGGTACTGGTGGTGAT
TACCCCGACGGTCATGCAGCAGCGCTTTGGTATCAGCGCCAGCGACACCTTTGCCCTGAGCGCCGTCGGCATCGTGTTTC
TCAATATAGGTTGTGTGCTGGCAGGCATGCTGGTCGACCGCATCGGCGCCTGGCGCGGCATCATGTTCTACAGCCTGCTG
ATGCCGATAGGCATCGGCGTGCTTTACACCAGCCTCATCGAGCAATGGATGCCACTGGGCATCGCCTATGCGATTGCCGG
GCTGGCGTGCGGGATTGTCGGTGTGGTGCCATCGGTGATGGTCGGCCTGTTCCCGGCGAACATCCGAGTGTCAGGCATCT
CGTTCACTTACAACATCGCCTACGCCTTCTGGGCCAGCACCACGCCATTGATGCTGATTACCCTGATGCCCTGGAATATC
TGGGTGTGCGTGTTCTATAGCCTGATCATGGGCGCCACGGGCCTGGTTACCGCAGCGGTATTCGGCCTGCGCCGGGGCGT
GGTGGTGGATGACCTGGCAAGCGGTAACGCCGGATAA

Upstream 100 bases:

>100_bases
GTTGCAGGCGTCAGCTCGGTATCAGTGCGCCCGCAAGCTTTGCTATTCTTCGCGCCCTGTGAGCGCCAGGCGCTCTTTCA
AGCCATGTTTCGGATGCCTC

Downstream 100 bases:

>100_bases
CTCCGGGCCGTGTGGCGCAACGCCCGCTGCTCACCTCTGTTGTGGGGTGAGTGGCAGCTCGAACATGGCCGGAAAGTTCC
TGCTGGCAGACGCGTTCATT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTDIVRSHSQPFSKADYKTLSLAALGGALEIYDFIIFVFFALTLSQLFFPPDMPEWLRLLQSFGIFVTGYLARPLGGILM
AHFADRLGRKRVFSLSILMMALPCLLIGVMPTYAQIGYWAPLVLLALRILQGAAVGGEVPSAWVFVAEHAPKGHRGYALG
VLQAGLTFGYLLGALTATWLARVFSPDQILEWAWRIPFLLGGVFGVVGVWLRRWLNETPVFIAMHAQREHLPALPLGQVL
RDHRQSLLPAALLTFVLTSAVVVLVVITPTVMQQRFGISASDTFALSAVGIVFLNIGCVLAGMLVDRIGAWRGIMFYSLL
MPIGIGVLYTSLIEQWMPLGIAYAIAGLACGIVGVVPSVMVGLFPANIRVSGISFTYNIAYAFWASTTPLMLITLMPWNI
WVCVFYSLIMGATGLVTAAVFGLRRGVVVDDLASGNAG

Sequences:

>Translated_438_residues
MTDIVRSHSQPFSKADYKTLSLAALGGALEIYDFIIFVFFALTLSQLFFPPDMPEWLRLLQSFGIFVTGYLARPLGGILM
AHFADRLGRKRVFSLSILMMALPCLLIGVMPTYAQIGYWAPLVLLALRILQGAAVGGEVPSAWVFVAEHAPKGHRGYALG
VLQAGLTFGYLLGALTATWLARVFSPDQILEWAWRIPFLLGGVFGVVGVWLRRWLNETPVFIAMHAQREHLPALPLGQVL
RDHRQSLLPAALLTFVLTSAVVVLVVITPTVMQQRFGISASDTFALSAVGIVFLNIGCVLAGMLVDRIGAWRGIMFYSLL
MPIGIGVLYTSLIEQWMPLGIAYAIAGLACGIVGVVPSVMVGLFPANIRVSGISFTYNIAYAFWASTTPLMLITLMPWNI
WVCVFYSLIMGATGLVTAAVFGLRRGVVVDDLASGNAG
>Mature_437_residues
TDIVRSHSQPFSKADYKTLSLAALGGALEIYDFIIFVFFALTLSQLFFPPDMPEWLRLLQSFGIFVTGYLARPLGGILMA
HFADRLGRKRVFSLSILMMALPCLLIGVMPTYAQIGYWAPLVLLALRILQGAAVGGEVPSAWVFVAEHAPKGHRGYALGV
LQAGLTFGYLLGALTATWLARVFSPDQILEWAWRIPFLLGGVFGVVGVWLRRWLNETPVFIAMHAQREHLPALPLGQVLR
DHRQSLLPAALLTFVLTSAVVVLVVITPTVMQQRFGISASDTFALSAVGIVFLNIGCVLAGMLVDRIGAWRGIMFYSLLM
PIGIGVLYTSLIEQWMPLGIAYAIAGLACGIVGVVPSVMVGLFPANIRVSGISFTYNIAYAFWASTTPLMLITLMPWNIW
VCVFYSLIMGATGLVTAAVFGLRRGVVVDDLASGNAG

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=30.0699300699301, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788292, Length=410, Percent_Identity=29.7560975609756, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1788942, Length=385, Percent_Identity=31.4285714285714, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789941, Length=435, Percent_Identity=27.816091954023, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082231, Length=428, Percent_Identity=22.6635514018692, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47614; Mature: 47483

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCEEECCCCCCCC
>Mature Secondary Structure 
TDIVRSHSQPFSKADYKTLSLAALGGALEIYDFIIFVFFALTLSQLFFPPDMPEWLRLL
CCHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
QSFGIFVTGYLARPLGGILMAHFADRLGRKRVFSLSILMMALPCLLIGVMPTYAQIGYWA
HHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLVLLALRILQGAAVGGEVPSAWVFVAEHAPKGHRGYALGVLQAGLTFGYLLGALTATWL
HHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARVFSPDQILEWAWRIPFLLGGVFGVVGVWLRRWLNETPVFIAMHAQREHLPALPLGQVL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHH
RDHRQSLLPAALLTFVLTSAVVVLVVITPTVMQQRFGISASDTFALSAVGIVFLNIGCVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AGMLVDRIGAWRGIMFYSLLMPIGIGVLYTSLIEQWMPLGIAYAIAGLACGIVGVVPSVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGLFPANIRVSGISFTYNIAYAFWASTTPLMLITLMPWNIWVCVFYSLIMGATGLVTAAV
HHHCCCCEEEEEEEEEEEHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHCCHHHHHHHH
FGLRRGVVVDDLASGNAG
HHHHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]