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The map label for this gene is yneJ [H]

Identifier: 66046829

GI number: 66046829

Start: 4281346

End: 4282314

Strand: Reverse

Name: yneJ [H]

Synonym: Psyr_3600

Alternate gene names: 66046829

Gene position: 4282314-4281346 (Counterclockwise)

Preceding gene: 66046834

Following gene: 66046827

Centisome position: 70.27

GC content: 61.4

Gene sequence:

>969_bases
ATGAGCGCCGGCTGGTACTATGCTTTTTGCCTATGTTTAATCTCTTTTTTATGTGCGGTCCGAATGAAAATTGATGATAT
CGATGCGTTCGTTGCAGTCATCCGCTGCCAGTCGATCAGCCACGCCGCTGAATCGCTGGATCTGACCCAGCCAGCCATCA
CGCGGCGGGTGCAGAATTTCGAGCAAGCCCTCGGAGTCGAGCTGTTCGACCGCAATACCAAGCCGCTCAAGCCGACTCCG
ATGGGGCTTCAGGTGTACCAGAAGTGCCTGGCGATCCTGCGCGAGATGGATTCGTTGCGCGAGCTGGTGGCCAGTGACAC
GCCGCCCAGCGGTCTGTTGCGTCTGGGCGTGCCGCAAACCATCGGTGACGTGGTGCTGCTCGATGCGCTCAAGCAATTGC
GTGGGGGATTTCCGGATCTGCGCGCCCAGGTCAGCACCGGTTGGGGCAGTCATCTGATCGGCAAGATCGAAAACGGCGAG
CTGGATGCGGCGGCTGCGCTGTTTCCGGCGGGCAAGATATTTCCGGACAACATCATCGGTCAGTCGATTGGCAAGATGGA
GCTGGTGGTGGTCTGCGCCAGGGGGCTGGCTCCGAAAAAGCCCGGCAAGCTGGCGGACTGTTATGAGCAGGGCTGGGTGC
TTAATCCGGACGGCTGCGGTTTTCGTGCAGGCTTGCAGCGCACGCTGACCGATCAGGGCTTGAGCATGAAGGTCAATCTG
GAAACTTTCGGCACCGAGCTGCAACTCGGGCTGGTGGCCGACGGCATGGGGCTTGGCCTGGTGCCCCGTCCGCTGCTGGA
ACGCAGCGCGCACCGCGAGCAGTTGGTGGCGTTGCCGCTCAAGGACTTCAAACCGGTGATGGACTTGTGGCTGTTCTACC
CGCGCTTCCTGGGTAACCTGCAAGCGCCGGTCGAAGCCTTTGGCGCACTGGTGGCCCGGTCGCTCAAGCCGGTCTCGGCC
GCCGCCTGA

Upstream 100 bases:

>100_bases
AATATGGATATTTAATATGCTATTTTTTAATGATTAGGTTATAACAAAAACGTCTTTTACTTCTTTGTCGTAATCCGCGA
AATCTACGCTCGGGCTATTA

Downstream 100 bases:

>100_bases
ACGCTGCATCGCGGGCAGGCGGTCTGGCGTTGGGCGTCAGACCGGCCCGCAGGGTATGGGTTTAATCAGGCGACCTGTTC
GCGAGCCTGCTGTTCACGCT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSAGWYYAFCLCLISFLCAVRMKIDDIDAFVAVIRCQSISHAAESLDLTQPAITRRVQNFEQALGVELFDRNTKPLKPTP
MGLQVYQKCLAILREMDSLRELVASDTPPSGLLRLGVPQTIGDVVLLDALKQLRGGFPDLRAQVSTGWGSHLIGKIENGE
LDAAAALFPAGKIFPDNIIGQSIGKMELVVVCARGLAPKKPGKLADCYEQGWVLNPDGCGFRAGLQRTLTDQGLSMKVNL
ETFGTELQLGLVADGMGLGLVPRPLLERSAHREQLVALPLKDFKPVMDLWLFYPRFLGNLQAPVEAFGALVARSLKPVSA
AA

Sequences:

>Translated_322_residues
MSAGWYYAFCLCLISFLCAVRMKIDDIDAFVAVIRCQSISHAAESLDLTQPAITRRVQNFEQALGVELFDRNTKPLKPTP
MGLQVYQKCLAILREMDSLRELVASDTPPSGLLRLGVPQTIGDVVLLDALKQLRGGFPDLRAQVSTGWGSHLIGKIENGE
LDAAAALFPAGKIFPDNIIGQSIGKMELVVVCARGLAPKKPGKLADCYEQGWVLNPDGCGFRAGLQRTLTDQGLSMKVNL
ETFGTELQLGLVADGMGLGLVPRPLLERSAHREQLVALPLKDFKPVMDLWLFYPRFLGNLQAPVEAFGALVARSLKPVSA
AA
>Mature_321_residues
SAGWYYAFCLCLISFLCAVRMKIDDIDAFVAVIRCQSISHAAESLDLTQPAITRRVQNFEQALGVELFDRNTKPLKPTPM
GLQVYQKCLAILREMDSLRELVASDTPPSGLLRLGVPQTIGDVVLLDALKQLRGGFPDLRAQVSTGWGSHLIGKIENGEL
DAAAALFPAGKIFPDNIIGQSIGKMELVVVCARGLAPKKPGKLADCYEQGWVLNPDGCGFRAGLQRTLTDQGLSMKVNLE
TFGTELQLGLVADGMGLGLVPRPLLERSAHREQLVALPLKDFKPVMDLWLFYPRFLGNLQAPVEAFGALVARSLKPVSAA
A

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=27.5261324041812, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787601, Length=292, Percent_Identity=24.3150684931507, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789506, Length=265, Percent_Identity=24.5283018867925, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788481, Length=279, Percent_Identity=24.7311827956989, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=25.177304964539, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082132, Length=246, Percent_Identity=23.5772357723577, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790399, Length=274, Percent_Identity=23.3576642335766, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693193, Length=168, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=246, Percent_Identity=25.609756097561, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=203, Percent_Identity=25.1231527093596, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=24.2914979757085, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=117, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34973; Mature: 34842

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGWYYAFCLCLISFLCAVRMKIDDIDAFVAVIRCQSISHAAESLDLTQPAITRRVQNF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EQALGVELFDRNTKPLKPTPMGLQVYQKCLAILREMDSLRELVASDTPPSGLLRLGVPQT
HHHHCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHH
IGDVVLLDALKQLRGGFPDLRAQVSTGWGSHLIGKIENGELDAAAALFPAGKIFPDNIIG
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHEECCCCCCCHHHHHCCCCCCCCHHHHH
QSIGKMELVVVCARGLAPKKPGKLADCYEQGWVLNPDGCGFRAGLQRTLTDQGLSMKVNL
HCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCEEEEEH
ETFGTELQLGLVADGMGLGLVPRPLLERSAHREQLVALPLKDFKPVMDLWLFYPRFLGNL
HHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCC
QAPVEAFGALVARSLKPVSAAA
CCHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SAGWYYAFCLCLISFLCAVRMKIDDIDAFVAVIRCQSISHAAESLDLTQPAITRRVQNF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EQALGVELFDRNTKPLKPTPMGLQVYQKCLAILREMDSLRELVASDTPPSGLLRLGVPQT
HHHHCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHH
IGDVVLLDALKQLRGGFPDLRAQVSTGWGSHLIGKIENGELDAAAALFPAGKIFPDNIIG
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHEECCCCCCCHHHHHCCCCCCCCHHHHH
QSIGKMELVVVCARGLAPKKPGKLADCYEQGWVLNPDGCGFRAGLQRTLTDQGLSMKVNL
HCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCEEEEEH
ETFGTELQLGLVADGMGLGLVPRPLLERSAHREQLVALPLKDFKPVMDLWLFYPRFLGNL
HHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCC
QAPVEAFGALVARSLKPVSAAA
CCHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]