Click here to switch to the map view.

The map label for this gene is rluB [H]

Identifier: 66046810

GI number: 66046810

Start: 4264625

End: 4265854

Strand: Reverse

Name: rluB [H]

Synonym: Psyr_3581

Alternate gene names: 66046810

Gene position: 4265854-4264625 (Counterclockwise)

Preceding gene: 66046811

Following gene: 66046809

Centisome position: 70.0

GC content: 63.74

Gene sequence:

>1230_bases
ATGAGTGAAAAAGACAAGCAGGACAGCCAGGAAATCGGACCCGCAGGCGAAAAGCTGCAGAAGGTTCTGGCGCGCATTGG
CGTGGGTTCACGCCGCGACGTGGAAGCCTGGATTACCCAAGGCCGTATCAAGGTCAACGGCAAGGACGCGACCCTCGGTC
AACGCGTCGACCTGCATGACGCCATCAGTGTCGATGGTCGCGTCATCAAGCGCGAAGAAGCGGCCGAAACGGTCCGTCGC
GTCATCATGTACAACAAGCCCGATGGTGAAATCTGCACCCGTGACGACCCTGAAGGCCGTCCGACCGTGTTTGATCGTCT
GCCGCGCCCGAAAGAAGGCCGCTGGATCAACATCGGTCGTCTCGACATCAATACCACCGGCCTGCTGATGTTCACCACCG
ACGGTGAACTCGCCAACCGCCTGATGCACCCGTCGTTTGAAATGGACCGTGAATACGCGGTTCGCGTGCGTGGCGAAGTC
GATGACGACATGCTGCTGCGCCTCAAGAACGGTGTGATTCTCGAAGACGGTCCTGCGCGCTTCACCGATATCCAGCAGGC
ACCGGGCGGCGAAGGCTTCAACCACTGGTATCACTGCGTGGTGATGGAAGGCCGCAACCGCGAAGTACGTCGTCTCTGGG
AATCCCAGGGGCTGGTGGTCAGCCGCCTGAAGCGCGTGCGTTACGGGCCGGTGTTCCTCAACTCCGACCTGCCGATGGGC
CGCTGGCGCGAAATGAGCCAGTACGAAGTCGATATTCTGGCCGCTGAAGTCGGTCTGCAACCTGTGGCCCAGCCGCAGAT
GAATGCCAAGACCAAGGACAAGATGGAGCGTCTGCAGCGTCAATCGTCGCGCCCTCTGGGCAAAGGCGAGCGCGTTCGCA
CCTTGCGTCCGGCTGCTGGTGCTCCGGCGACCGGCGGCGAGCGCGCGCCGCGTCAGCCGCAGATCACCGGCAGCGAGCGT
GATCGTCCGCGCGGTGGCGATTCGACTCAGCGTCCTTCGCCGCGCAAGGATTCGGGCAAGCCCGGTTCGCGCGCGCCGCG
TCCGGCCGACAACAGCCGTGGCACGCCGGTCGCCGAGCGTCCGAGCGACATGAACAAGCGTCCGGCCAAGCCGGGCCCCA
AGCGTGTCGGCATCAAGCTGGTCAACGATGATGCGCCGTCAGGCAAGCGTCGTGGCCCACCGGCAGGTTCAGGTCAGCGT
CCGGGCTTCGGTCGTCGCAAGCCCGAGTGA

Upstream 100 bases:

>100_bases
GGCTTTCAAGTATTTCGCCTTGCCGCTATCCACAGCGCGCCGGGCACAGGTCTTGCCGGTTCTCGGCCAGCCCTGATTCG
ACACACCGGGAGGTGCCCAG

Downstream 100 bases:

>100_bases
TCGCGCGGTGTGAATGAATGGCAGCGCCAGTCCACAGGGCTGGCGTTGTCGTTTTCGGCGCTCTGAAAAGTGCGGTCACA
CAGCCGCGGCACCTCGACCC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSEKDKQDSQEIGPAGEKLQKVLARIGVGSRRDVEAWITQGRIKVNGKDATLGQRVDLHDAISVDGRVIKREEAAETVRR
VIMYNKPDGEICTRDDPEGRPTVFDRLPRPKEGRWINIGRLDINTTGLLMFTTDGELANRLMHPSFEMDREYAVRVRGEV
DDDMLLRLKNGVILEDGPARFTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRYGPVFLNSDLPMG
RWREMSQYEVDILAAEVGLQPVAQPQMNAKTKDKMERLQRQSSRPLGKGERVRTLRPAAGAPATGGERAPRQPQITGSER
DRPRGGDSTQRPSPRKDSGKPGSRAPRPADNSRGTPVAERPSDMNKRPAKPGPKRVGIKLVNDDAPSGKRRGPPAGSGQR
PGFGRRKPE

Sequences:

>Translated_409_residues
MSEKDKQDSQEIGPAGEKLQKVLARIGVGSRRDVEAWITQGRIKVNGKDATLGQRVDLHDAISVDGRVIKREEAAETVRR
VIMYNKPDGEICTRDDPEGRPTVFDRLPRPKEGRWINIGRLDINTTGLLMFTTDGELANRLMHPSFEMDREYAVRVRGEV
DDDMLLRLKNGVILEDGPARFTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRYGPVFLNSDLPMG
RWREMSQYEVDILAAEVGLQPVAQPQMNAKTKDKMERLQRQSSRPLGKGERVRTLRPAAGAPATGGERAPRQPQITGSER
DRPRGGDSTQRPSPRKDSGKPGSRAPRPADNSRGTPVAERPSDMNKRPAKPGPKRVGIKLVNDDAPSGKRRGPPAGSGQR
PGFGRRKPE
>Mature_408_residues
SEKDKQDSQEIGPAGEKLQKVLARIGVGSRRDVEAWITQGRIKVNGKDATLGQRVDLHDAISVDGRVIKREEAAETVRRV
IMYNKPDGEICTRDDPEGRPTVFDRLPRPKEGRWINIGRLDINTTGLLMFTTDGELANRLMHPSFEMDREYAVRVRGEVD
DDMLLRLKNGVILEDGPARFTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRYGPVFLNSDLPMGR
WREMSQYEVDILAAEVGLQPVAQPQMNAKTKDKMERLQRQSSRPLGKGERVRTLRPAAGAPATGGERAPRQPQITGSERD
RPRGGDSTQRPSPRKDSGKPGSRAPRPADNSRGTPVAERPSDMNKRPAKPGPKRVGIKLVNDDAPSGKRRGPPAGSGQRP
GFGRRKPE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=291, Percent_Identity=53.2646048109966, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=32.0512820512821, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788510, Length=176, Percent_Identity=32.3863636363636, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081838, Length=188, Percent_Identity=32.4468085106383, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 45684; Mature: 45553

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKDKQDSQEIGPAGEKLQKVLARIGVGSRRDVEAWITQGRIKVNGKDATLGQRVDLHD
CCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCEEEECCCCCCCCCCCCHHH
AISVDGRVIKREEAAETVRRVIMYNKPDGEICTRDDPEGRPTVFDRLPRPKEGRWINIGR
HHCCCCEEECHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEE
LDINTTGLLMFTTDGELANRLMHPSFEMDREYAVRVRGEVDDDMLLRLKNGVILEDGPAR
EEECCCEEEEEECCHHHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCC
FTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRYGPVFLNSDLPMG
HHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCHH
RWREMSQYEVDILAAEVGLQPVAQPQMNAKTKDKMERLQRQSSRPLGKGERVRTLRPAAG
HHHHCCHHHEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCC
APATGGERAPRQPQITGSERDRPRGGDSTQRPSPRKDSGKPGSRAPRPADNSRGTPVAER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PSDMNKRPAKPGPKRVGIKLVNDDAPSGKRRGPPAGSGQRPGFGRRKPE
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKDKQDSQEIGPAGEKLQKVLARIGVGSRRDVEAWITQGRIKVNGKDATLGQRVDLHD
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCEEEECCCCCCCCCCCCHHH
AISVDGRVIKREEAAETVRRVIMYNKPDGEICTRDDPEGRPTVFDRLPRPKEGRWINIGR
HHCCCCEEECHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEE
LDINTTGLLMFTTDGELANRLMHPSFEMDREYAVRVRGEVDDDMLLRLKNGVILEDGPAR
EEECCCEEEEEECCHHHHHHHCCCCHHCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCC
FTDIQQAPGGEGFNHWYHCVVMEGRNREVRRLWESQGLVVSRLKRVRYGPVFLNSDLPMG
HHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCHH
RWREMSQYEVDILAAEVGLQPVAQPQMNAKTKDKMERLQRQSSRPLGKGERVRTLRPAAG
HHHHCCHHHEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCC
APATGGERAPRQPQITGSERDRPRGGDSTQRPSPRKDSGKPGSRAPRPADNSRGTPVAER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PSDMNKRPAKPGPKRVGIKLVNDDAPSGKRRGPPAGSGQRPGFGRRKPE
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]