| Definition | Pseudomonas syringae pv. syringae B728a, complete genome. |
|---|---|
| Accession | NC_007005 |
| Length | 6,093,698 |
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The map label for this gene is kefA [H]
Identifier: 66046778
GI number: 66046778
Start: 4226391
End: 4228802
Strand: Direct
Name: kefA [H]
Synonym: Psyr_3549
Alternate gene names: 66046778
Gene position: 4226391-4228802 (Clockwise)
Preceding gene: 66046775
Following gene: 66046779
Centisome position: 69.36
GC content: 61.32
Gene sequence:
>2412_bases ATGCAGCACGCTTCGCTGAACCGTTTTCGTACCGGGTTGCTCGCCTTGCTTTTGCTGTGCGTGACCTTGCCGGCCCTGGC CCAGAATGCCCCCGCTCCCCAGGCCGATAGTGCCAGCAGCGCAGCGCCTGCCGTGGAAGCCCCGAGCCTGGATGATCTGA ACCAACAGCTCGATCAGATCCGTCAGAAAGTGACGGTCAGCGCCAACGACGACCTGCTTTCAAGCCTGCGCCAGGCGGCT TTGCAGGTCCAGAAACAAGCCGACAATCTGGTTGCGCAGCAGGCTGTCGACATCGAACACCTGAATGATCAGCTCAACAT TCTCGGGCCGGTGCAACCGGACGAAGCGCCAAGCCTGACCAGTCAGCGCAAGACATTGACCGCGCAGAAAAACGCGCTGG TCAATGATGAACGGCAGACCAATGAGCTAAGTCAGTCAGCCCGGGATCTGGCTACGCAGATATTCAACCTGCGCCGCAGC CTCTTCGATTCACAGATCAGCACGCGCACCGCGAGCCCGCTCAGTTCGGCATTCTGGTCCACGCTGATTCGCCCGACCGA TGACGACCTGGGACGGTTGAACAGGCTTCTGGTGGACGTACGCCAGGTACTCAACAACGCGATGGCACCGGACAACCGTG TGCAATTCATCAGCGTGGTGCTGGGCGCGTTGTTCATCTGGGTGGTTGTCCGGCGCCTGCTGGAGCGCCTGCTGATCTGG GCAATGATTCGCTGGCTGCCCGAAGGCCGTCTGCGCCGCAGTGCTCTGGCCCTGGCTGTCGGGCTGTCGACCATTCTCAC CATCACCATTGCCACTTCCTTGCTGCGCTGGGGGATCGTCCACAACGCGGTACTCAGCAACGACGTAGTCAATCTGCTCG ATCAGGTGCAGACGCTGATTACCTTCTGCGCCTTCATCGTCGGTCTGGGCCGTGCGCTGTTGATGCTGGCACACGCCTCG TGGCGCCTGCCGCAAATTCCTGACCAGATCGCCATCGCATTGGGTCGCTTCCCCCCTGCCCTGGCGCTGGCACTGATGGT CATCGGCACGCAGGAACGAATCAACAGCGTGATTGCCAGCAGCCTGGCCCTGACGGTTGCGGTCAATGGCCTGACGGCAC TGGCGGTTTCGCTGGTGTTCTTCTATGCCCTGTTGCGCTACCGCCGCACCCGCCGACGCTTTGAGCTGGAGCGCCCGGCG GGCTTCGCCGGGCTGATTCCGTTTATCGTTGCGGTGTGGGTCGGCCTGAGTTTGCTGGCGTTGTTGAGCGGCTACCTGAC GCTGGCGTACTTTCTGGCAGTGAAACTGCTCTGGGTGAGCGTGGTAGCGTCCACTGCATACCTGCTGATCGCCTGCTTCG GTGACATCTGCGAAACCCTCTTGTCGCCCAAGCAACCCGGCGGCCTGGCGCTGGGTTCCGCGCTGGGCTTGTCCACGCGC CATCAGGCGCAGGCAAGTACCGTGCTGGCCGGCATAGGCCGCACCTTGCTGCTGCTCACCGCTGTACTGCTGGCGTTCCT GCCTTCAGGGTCGAGCCCGGGCGACCTGCTCGCCAGCTTTACTCAGCTGGATGTCACCTCCAAATCCATGGGCAACCTGA ACATCGTCCCCGGCGACATTCTGGTAGCACTGGTGTGCCTGGTCGTCGGCCTGCTGAGCGTGCGTGTCCTCAAGGAGTGG TTTGGAGAACGCCTGCTGCCCGAAACCAACATGGACGCGGGCATGCAGGCCTCGCTGGTGACCCTGATCGGATACATCGG CTTTGTGCTGGTGGTGGCCGTGGTGATGTCCACACTCAACATCAGCCTTACCAACCTGACCTGGGTGGTCAGTGCGCTGT CGGTGGGGATAGGTTTCGGCCTGCAAGCCATTGTGCAGAACTTCATCTCCGGCCTGATTCTGCTGACCGAGCGTCCGGTC AAAGTCGGCGACTGGGTAAGCCTGGCGGGGGTAGAAGGCGATATTCGCCGGATCAACGTCCGCGCGACCGAAATCCAGAT GGGCGACCGCTCGACAGTGATCGTGCCGAACTCGCAATTCATCACGCAGAACGTGCGCAACGTGACCATGGGCAACGCGC TGGGGGTGGTCGGAATCACCCTTACCCTGCCCCTGGAAACAGACGTTCTGCAAATACGCGAACTGCTGTTGCAGGCCTTC ACCGAGCATGAAGCCATACTCGATGCGCCAGCGCCTTCAGTCACCTTCAAGGACTTGACCAATACCGGTTTGATCATCAG CGCCAGCGGCTACGTGAACAGCCCACGCTCTGTGGGTGGCGCACGGAGTGATCTGTTGTTTACCGTTCTGGGGCGTCTGA GGGAATTGGGTGTTGCGCTGTCTGCACCGCAGAGCATGGTGTTGATCAATGAGGGGGCTGGGAAGGAGGCGGCGGCGGAG GAGAGTCAGTGA
Upstream 100 bases:
>100_bases GCTGATCTCGGGCGGCCAGTGCATGCCGCTCAAACACAAGACCCAGACTCGAACCACAGGCCTCGCCAGGCCGCATCGCG CAGAGAAAGAAGGAAAAAGA
Downstream 100 bases:
>100_bases TGCTTGTTTATATGGTGTCCCAGGCGGGGTTCGAACCTGCAACCTTCCCCTTAGGAGGGGGATGCTCTATCCAATTGAGC TACTGAGACACTGCGTGCCG
Product: MscS mechanosensitive ion channel
Products: NA
Alternate protein names: Protein AefA [H]
Number of amino acids: Translated: 803; Mature: 803
Protein sequence:
>803_residues MQHASLNRFRTGLLALLLLCVTLPALAQNAPAPQADSASSAAPAVEAPSLDDLNQQLDQIRQKVTVSANDDLLSSLRQAA LQVQKQADNLVAQQAVDIEHLNDQLNILGPVQPDEAPSLTSQRKTLTAQKNALVNDERQTNELSQSARDLATQIFNLRRS LFDSQISTRTASPLSSAFWSTLIRPTDDDLGRLNRLLVDVRQVLNNAMAPDNRVQFISVVLGALFIWVVVRRLLERLLIW AMIRWLPEGRLRRSALALAVGLSTILTITIATSLLRWGIVHNAVLSNDVVNLLDQVQTLITFCAFIVGLGRALLMLAHAS WRLPQIPDQIAIALGRFPPALALALMVIGTQERINSVIASSLALTVAVNGLTALAVSLVFFYALLRYRRTRRRFELERPA GFAGLIPFIVAVWVGLSLLALLSGYLTLAYFLAVKLLWVSVVASTAYLLIACFGDICETLLSPKQPGGLALGSALGLSTR HQAQASTVLAGIGRTLLLLTAVLLAFLPSGSSPGDLLASFTQLDVTSKSMGNLNIVPGDILVALVCLVVGLLSVRVLKEW FGERLLPETNMDAGMQASLVTLIGYIGFVLVVAVVMSTLNISLTNLTWVVSALSVGIGFGLQAIVQNFISGLILLTERPV KVGDWVSLAGVEGDIRRINVRATEIQMGDRSTVIVPNSQFITQNVRNVTMGNALGVVGITLTLPLETDVLQIRELLLQAF TEHEAILDAPAPSVTFKDLTNTGLIISASGYVNSPRSVGGARSDLLFTVLGRLRELGVALSAPQSMVLINEGAGKEAAAE ESQ
Sequences:
>Translated_803_residues MQHASLNRFRTGLLALLLLCVTLPALAQNAPAPQADSASSAAPAVEAPSLDDLNQQLDQIRQKVTVSANDDLLSSLRQAA LQVQKQADNLVAQQAVDIEHLNDQLNILGPVQPDEAPSLTSQRKTLTAQKNALVNDERQTNELSQSARDLATQIFNLRRS LFDSQISTRTASPLSSAFWSTLIRPTDDDLGRLNRLLVDVRQVLNNAMAPDNRVQFISVVLGALFIWVVVRRLLERLLIW AMIRWLPEGRLRRSALALAVGLSTILTITIATSLLRWGIVHNAVLSNDVVNLLDQVQTLITFCAFIVGLGRALLMLAHAS WRLPQIPDQIAIALGRFPPALALALMVIGTQERINSVIASSLALTVAVNGLTALAVSLVFFYALLRYRRTRRRFELERPA GFAGLIPFIVAVWVGLSLLALLSGYLTLAYFLAVKLLWVSVVASTAYLLIACFGDICETLLSPKQPGGLALGSALGLSTR HQAQASTVLAGIGRTLLLLTAVLLAFLPSGSSPGDLLASFTQLDVTSKSMGNLNIVPGDILVALVCLVVGLLSVRVLKEW FGERLLPETNMDAGMQASLVTLIGYIGFVLVVAVVMSTLNISLTNLTWVVSALSVGIGFGLQAIVQNFISGLILLTERPV KVGDWVSLAGVEGDIRRINVRATEIQMGDRSTVIVPNSQFITQNVRNVTMGNALGVVGITLTLPLETDVLQIRELLLQAF TEHEAILDAPAPSVTFKDLTNTGLIISASGYVNSPRSVGGARSDLLFTVLGRLRELGVALSAPQSMVLINEGAGKEAAAE ESQ >Mature_803_residues MQHASLNRFRTGLLALLLLCVTLPALAQNAPAPQADSASSAAPAVEAPSLDDLNQQLDQIRQKVTVSANDDLLSSLRQAA LQVQKQADNLVAQQAVDIEHLNDQLNILGPVQPDEAPSLTSQRKTLTAQKNALVNDERQTNELSQSARDLATQIFNLRRS LFDSQISTRTASPLSSAFWSTLIRPTDDDLGRLNRLLVDVRQVLNNAMAPDNRVQFISVVLGALFIWVVVRRLLERLLIW AMIRWLPEGRLRRSALALAVGLSTILTITIATSLLRWGIVHNAVLSNDVVNLLDQVQTLITFCAFIVGLGRALLMLAHAS WRLPQIPDQIAIALGRFPPALALALMVIGTQERINSVIASSLALTVAVNGLTALAVSLVFFYALLRYRRTRRRFELERPA GFAGLIPFIVAVWVGLSLLALLSGYLTLAYFLAVKLLWVSVVASTAYLLIACFGDICETLLSPKQPGGLALGSALGLSTR HQAQASTVLAGIGRTLLLLTAVLLAFLPSGSSPGDLLASFTQLDVTSKSMGNLNIVPGDILVALVCLVVGLLSVRVLKEW FGERLLPETNMDAGMQASLVTLIGYIGFVLVVAVVMSTLNISLTNLTWVVSALSVGIGFGLQAIVQNFISGLILLTERPV KVGDWVSLAGVEGDIRRINVRATEIQMGDRSTVIVPNSQFITQNVRNVTMGNALGVVGITLTLPLETDVLQIRELLLQAF TEHEAILDAPAPSVTFKDLTNTGLIISASGYVNSPRSVGGARSDLLFTVLGRLRELGVALSAPQSMVLINEGAGKEAAAE ESQ
Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form
COG id: COG3264
COG function: function code M; Small-conductance mechanosensitive channel
Gene ontology:
Cell location: Cell inner membrane; Multi-pass membrane protein [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the mscS (TC 1.A.23) family [H]
Homologues:
Organism=Escherichia coli, GI1786670, Length=292, Percent_Identity=31.1643835616438, Blast_Score=141, Evalue=1e-34, Organism=Escherichia coli, GI2367355, Length=308, Percent_Identity=29.5454545454545, Blast_Score=134, Evalue=3e-32, Organism=Escherichia coli, GI1789291, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=3e-13,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR010920 - InterPro: IPR011066 - InterPro: IPR006685 - InterPro: IPR006686 - InterPro: IPR011014 [H]
Pfam domain/function: PF00924 MS_channel [H]
EC number: NA
Molecular weight: Translated: 86354; Mature: 86354
Theoretical pI: Translated: 7.90; Mature: 7.90
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQHASLNRFRTGLLALLLLCVTLPALAQNAPAPQADSASSAAPAVEAPSLDDLNQQLDQI CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH RQKVTVSANDDLLSSLRQAALQVQKQADNLVAQQAVDIEHLNDQLNILGPVQPDEAPSLT HHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH SQRKTLTAQKNALVNDERQTNELSQSARDLATQIFNLRRSLFDSQISTRTASPLSSAFWS HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH TLIRPTDDDLGRLNRLLVDVRQVLNNAMAPDNRVQFISVVLGALFIWVVVRRLLERLLIW HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH AMIRWLPEGRLRRSALALAVGLSTILTITIATSLLRWGIVHNAVLSNDVVNLLDQVQTLI HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH TFCAFIVGLGRALLMLAHASWRLPQIPDQIAIALGRFPPALALALMVIGTQERINSVIAS HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHH SLALTVAVNGLTALAVSLVFFYALLRYRRTRRRFELERPAGFAGLIPFIVAVWVGLSLLA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH LLSGYLTLAYFLAVKLLWVSVVASTAYLLIACFGDICETLLSPKQPGGLALGSALGLSTR HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCHH HQAQASTVLAGIGRTLLLLTAVLLAFLPSGSSPGDLLASFTQLDVTSKSMGNLNIVPGDI HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHCCCCCEECCHHH LVALVCLVVGLLSVRVLKEWFGERLLPETNMDAGMQASLVTLIGYIGFVLVVAVVMSTLN HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC ISLTNLTWVVSALSVGIGFGLQAIVQNFISGLILLTERPVKVGDWVSLAGVEGDIRRINV CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCCCCEEEEE RATEIQMGDRSTVIVPNSQFITQNVRNVTMGNALGVVGITLTLPLETDVLQIRELLLQAF EEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHH TEHEAILDAPAPSVTFKDLTNTGLIISASGYVNSPRSVGGARSDLLFTVLGRLRELGVAL HHHHHHHCCCCCCCCHHHCCCCCEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHH SAPQSMVLINEGAGKEAAAEESQ CCCCEEEEEECCCCCCCCCCCCC >Mature Secondary Structure MQHASLNRFRTGLLALLLLCVTLPALAQNAPAPQADSASSAAPAVEAPSLDDLNQQLDQI CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH RQKVTVSANDDLLSSLRQAALQVQKQADNLVAQQAVDIEHLNDQLNILGPVQPDEAPSLT HHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH SQRKTLTAQKNALVNDERQTNELSQSARDLATQIFNLRRSLFDSQISTRTASPLSSAFWS HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH TLIRPTDDDLGRLNRLLVDVRQVLNNAMAPDNRVQFISVVLGALFIWVVVRRLLERLLIW HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH AMIRWLPEGRLRRSALALAVGLSTILTITIATSLLRWGIVHNAVLSNDVVNLLDQVQTLI HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH TFCAFIVGLGRALLMLAHASWRLPQIPDQIAIALGRFPPALALALMVIGTQERINSVIAS HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHH SLALTVAVNGLTALAVSLVFFYALLRYRRTRRRFELERPAGFAGLIPFIVAVWVGLSLLA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH LLSGYLTLAYFLAVKLLWVSVVASTAYLLIACFGDICETLLSPKQPGGLALGSALGLSTR HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCHH HQAQASTVLAGIGRTLLLLTAVLLAFLPSGSSPGDLLASFTQLDVTSKSMGNLNIVPGDI HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHCCCCCEECCHHH LVALVCLVVGLLSVRVLKEWFGERLLPETNMDAGMQASLVTLIGYIGFVLVVAVVMSTLN HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC ISLTNLTWVVSALSVGIGFGLQAIVQNFISGLILLTERPVKVGDWVSLAGVEGDIRRINV CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCCCCEEEEE RATEIQMGDRSTVIVPNSQFITQNVRNVTMGNALGVVGITLTLPLETDVLQIRELLLQAF EEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHH TEHEAILDAPAPSVTFKDLTNTGLIISASGYVNSPRSVGGARSDLLFTVLGRLRELGVAL HHHHHHHCCCCCCCCHHHCCCCCEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHH SAPQSMVLINEGAGKEAAAEESQ CCCCEEEEEECCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9278503; 10202137 [H]