BacMap

Definition	Pseudomonas syringae pv. syringae B728a, complete genome.
Accession	NC_007005
Length	6,093,698

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The map label for this gene is ygfF [C]

Identifier: 66046771

GI number: 66046771

Start: 4219845

End: 4220594

Strand: Reverse

Name: ygfF [C]

Synonym: Psyr_3542

Alternate gene names: 66046771

Gene position: 4220594-4219845 (Counterclockwise)

Preceding gene: 66046773

Following gene: 66046766

Centisome position: 69.26

GC content: 60.93

Gene sequence:

>750_bases
ATGAAACTCGAAGGAAAGATAGCGGTTGTCACCGGGGCGTCCAAAGGCATAGGTGCCGGGATTGCCAAAGCATTGGGCGC
AGAGGGCGCAAGGGTCATCGTCAATTACGCCACCGGCAAGGCGGATGCGGACGCCGTCGTCGCCTGGATCGCAGAGCACG
GCGGCTCCGCGTTTGCGGTACAGGCAGACATGAGCCAGTCAGCAGACGTGATACGCCTGTTCGAAACGGTCGGCACGAAG
TACGGCGCTCTGGACATTCTGGTCAACAATGCGGGTGTCGCTGTGTTCCAGATGATCGACGACCTGACTGAAGAGGCTTT
CCACACGCAATTCAACCTGAACGTGCTGGGCTACCTGCTTGCAGTGCGAGAGGCCGTCAAATTGTTGGGGCCGACCGGCA
GCATCATCAATATCAGCTCGATTCTCAGCACCGACCCTTACCTGGCGTCGAGCGTGTACGCGGCGACCAAGGGCGCGGTC
GATACCTTGACCTTCGCGCTGGCCAGAGAGCTGGGCGCGCGGGGCATCAGGGTCAACTCGATCCTGCCGGGGCACACCAA
CACCCCGGCAACCCATGGGCATTTTGCCGGTGAGTTAGGTGAGAAGATTCTGGCTGGAACACCCTTGGGTCGTTTTGGAG
AGCCCGAAGATATCGCCCCCTTGGCTGTGTTTCTGGCGTCGCAGGACTCGCACTGGGTCACCGGCGAATCCATCCGCGCT
TCGGGCGGGGTCAGGGGCGTCGGTTACTGA

Upstream 100 bases:

>100_bases
GATGGCCGAATACTGATGACACGCGGCTGGTTGCCTGGCGGTCATTCATGATCCGACTGCAACGAGGCCTCATACCACAC
CACTTCAAGAGGGCCGGTTT

Downstream 100 bases:

>100_bases
CGCTATCGGTTGAACCAAGGATGCGCATGGACCGGAGAGGGCGCTTTGGCAACTTACCATTCCATCCCGACCCATGCCAT
TTACTCCGGCAAGCGAAACT

Product: Short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKLEGKIAVVTGASKGIGAGIAKALGAEGARVIVNYATGKADADAVVAWIAEHGGSAFAVQADMSQSADVIRLFETVGTK
YGALDILVNNAGVAVFQMIDDLTEEAFHTQFNLNVLGYLLAVREAVKLLGPTGSIINISSILSTDPYLASSVYAATKGAV
DTLTFALARELGARGIRVNSILPGHTNTPATHGHFAGELGEKILAGTPLGRFGEPEDIAPLAVFLASQDSHWVTGESIRA
SGGVRGVGY

Sequences:

>Translated_249_residues
MKLEGKIAVVTGASKGIGAGIAKALGAEGARVIVNYATGKADADAVVAWIAEHGGSAFAVQADMSQSADVIRLFETVGTK
YGALDILVNNAGVAVFQMIDDLTEEAFHTQFNLNVLGYLLAVREAVKLLGPTGSIINISSILSTDPYLASSVYAATKGAV
DTLTFALARELGARGIRVNSILPGHTNTPATHGHFAGELGEKILAGTPLGRFGEPEDIAPLAVFLASQDSHWVTGESIRA
SGGVRGVGY
>Mature_249_residues
MKLEGKIAVVTGASKGIGAGIAKALGAEGARVIVNYATGKADADAVVAWIAEHGGSAFAVQADMSQSADVIRLFETVGTK
YGALDILVNNAGVAVFQMIDDLTEEAFHTQFNLNVLGYLLAVREAVKLLGPTGSIINISSILSTDPYLASSVYAATKGAV
DTLTFALARELGARGIRVNSILPGHTNTPATHGHFAGELGEKILAGTPLGRFGEPEDIAPLAVFLASQDSHWVTGESIRA
SGGVRGVGY

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=254, Percent_Identity=35.0393700787402, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI32483357, Length=245, Percent_Identity=34.2857142857143, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI7705925, Length=245, Percent_Identity=35.5102040816327, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=31.8367346938775, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=30.241935483871, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=30.4526748971193, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI59889578, Length=235, Percent_Identity=33.6170212765957, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI126723750, Length=248, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI19923817, Length=251, Percent_Identity=27.0916334661355, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=30.4, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI209862901, Length=222, Percent_Identity=34.2342342342342, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI19923983, Length=222, Percent_Identity=34.2342342342342, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4758504, Length=263, Percent_Identity=28.1368821292776, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI31542939, Length=208, Percent_Identity=31.25, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=32.4175824175824, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI33667109, Length=188, Percent_Identity=29.7872340425532, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI83715985, Length=259, Percent_Identity=25.8687258687259, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI10190704, Length=246, Percent_Identity=27.2357723577236, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI4504505, Length=258, Percent_Identity=26.3565891472868, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=39.8373983739837, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=34.9397590361446, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787335, Length=246, Percent_Identity=32.9268292682927, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789378, Length=247, Percent_Identity=36.0323886639676, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=34.2307692307692, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789208, Length=246, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082160, Length=247, Percent_Identity=31.5789473684211, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790717, Length=245, Percent_Identity=30.2040816326531, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI2367365, Length=235, Percent_Identity=33.1914893617021, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788459, Length=242, Percent_Identity=31.404958677686, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789057, Length=254, Percent_Identity=31.8897637795276, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786812, Length=255, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787545, Length=250, Percent_Identity=27.6, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788891, Length=240, Percent_Identity=29.5833333333333, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787820, Length=231, Percent_Identity=28.1385281385281, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787526, Length=251, Percent_Identity=27.8884462151394, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=246, Percent_Identity=34.9593495934959, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=36.3265306122449, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=32.5842696629214, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=246, Percent_Identity=32.1138211382114, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115534694, Length=242, Percent_Identity=31.8181818181818, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17561402, Length=258, Percent_Identity=34.4961240310078, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17544670, Length=264, Percent_Identity=28.4090909090909, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI72000259, Length=262, Percent_Identity=32.824427480916, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17560332, Length=269, Percent_Identity=32.7137546468402, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17563726, Length=271, Percent_Identity=31.3653136531365, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=33.7121212121212, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560150, Length=269, Percent_Identity=33.0855018587361, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17538182, Length=263, Percent_Identity=31.1787072243346, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI193204405, Length=261, Percent_Identity=32.5670498084291, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17562906, Length=270, Percent_Identity=32.2222222222222, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=266, Percent_Identity=31.9548872180451, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=27.8884462151394, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531453, Length=265, Percent_Identity=31.6981132075472, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=267, Percent_Identity=31.4606741573034, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=259, Percent_Identity=31.2741312741313, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17562904, Length=272, Percent_Identity=30.8823529411765, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17561272, Length=208, Percent_Identity=32.2115384615385, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=265, Percent_Identity=29.811320754717, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536025, Length=251, Percent_Identity=29.4820717131474, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=265, Percent_Identity=30.5660377358491, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17551412, Length=266, Percent_Identity=29.3233082706767, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=30.3030303030303, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536651, Length=251, Percent_Identity=28.2868525896414, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17555612, Length=220, Percent_Identity=31.8181818181818, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=275, Percent_Identity=29.4545454545455, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508651, Length=255, Percent_Identity=29.0196078431373, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI86565303, Length=217, Percent_Identity=29.9539170506912, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71997099, Length=209, Percent_Identity=32.0574162679426, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=192, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=256, Percent_Identity=26.953125, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=251, Percent_Identity=27.4900398406374, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323882, Length=248, Percent_Identity=27.8225806451613, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=254, Percent_Identity=27.5590551181102, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322227, Length=219, Percent_Identity=26.4840182648402, Blast_Score=65, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=35.0806451612903, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=32.5396825396825, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI23397609, Length=250, Percent_Identity=33.2, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24643142, Length=242, Percent_Identity=35.5371900826446, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI28571526, Length=253, Percent_Identity=32.8063241106719, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=32.4, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357041, Length=248, Percent_Identity=33.8709677419355, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17737361, Length=263, Percent_Identity=30.4182509505703, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644337, Length=206, Percent_Identity=30.0970873786408, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24641232, Length=200, Percent_Identity=30.5, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357673, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281364494, Length=204, Percent_Identity=30.3921568627451, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24663911, Length=197, Percent_Identity=26.9035532994924, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641388, Length=230, Percent_Identity=29.5652173913043, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665243, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221331218, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648944, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24665247, Length=195, Percent_Identity=27.6923076923077, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 25677; Mature: 25677

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEGKIAVVTGASKGIGAGIAKALGAEGARVIVNYATGKADADAVVAWIAEHGGSAFAV
CCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEE
QADMSQSADVIRLFETVGTKYGALDILVNNAGVAVFQMIDDLTEEAFHTQFNLNVLGYLL
ECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AVREAVKLLGPTGSIINISSILSTDPYLASSVYAATKGAVDTLTFALARELGARGIRVNS
HHHHHHHHHCCCCCEEEHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEE
ILPGHTNTPATHGHFAGELGEKILAGTPLGRFGEPEDIAPLAVFLASQDSHWVTGESIRA
ECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCEEC
SGGVRGVGY
CCCCCCCCC
>Mature Secondary Structure
MKLEGKIAVVTGASKGIGAGIAKALGAEGARVIVNYATGKADADAVVAWIAEHGGSAFAV
CCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEE
QADMSQSADVIRLFETVGTKYGALDILVNNAGVAVFQMIDDLTEEAFHTQFNLNVLGYLL
ECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AVREAVKLLGPTGSIINISSILSTDPYLASSVYAATKGAVDTLTFALARELGARGIRVNS
HHHHHHHHHCCCCCEEEHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEE
ILPGHTNTPATHGHFAGELGEKILAGTPLGRFGEPEDIAPLAVFLASQDSHWVTGESIRA
ECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCEEC
SGGVRGVGY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]