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The map label for this gene is gntR [H]

Identifier: 66046565

GI number: 66046565

Start: 3991328

End: 3992344

Strand: Reverse

Name: gntR [H]

Synonym: Psyr_3336

Alternate gene names: 66046565

Gene position: 3992344-3991328 (Counterclockwise)

Preceding gene: 66046569

Following gene: 66046564

Centisome position: 65.52

GC content: 64.31

Gene sequence:

>1017_bases
ATGATACGCATTGGTTCCCGCTCGACCGGTCGCCCCACGCTCAATGAAGTGGCGAAGCTGGCTGGCGTCTCTCCGATCAC
CGCTTCGCGTGCGTTGCGCGGGATCAGCACCGTAGCGCCTGAACTCGTCGAAAAGGTCCGCGCTGCGGCGCAGAGCCTGG
GTTATGTAGCCAACCCGGCAGCCAGGGCGCTGGCTTCTTCCTGCAGTCAGACCGTCGTGGTGCTGGTGCCATCGCTTTCC
AACCAGCTGTTCATCGAGACGCTGGAAGCCATTCAGGACGTGTTGCGCGTGCGTGGCCTGGACGTGCTGATCGGCAACTA
CCATTACTCGCCCGAAGAGGAAGAGAAGCTGCTGCGCAACCATCTGGTCAATCGCCCGCGCGGTATTCTGCTGACCGGTT
TCGACCACACGGCAGCGTCCCGCCAGATGCTTGAAACCAGTGGTATACCCTGCGTGCACATGATGGAACTCGACACCCGC
CTGGGCGCTTATTGCGTCGGATTCTCGCAACAACAGGCCGGCGCCGAAGCCGCGCATCACCTGCTCGGGCGTGGCCGCCG
TCGGCTGGCGTACATGGCCGCCCAGCTCGACCCTCGGGTACTGCAACGTGGCGAAGGGTTCCGTCGAGTGCTGGAAAGCG
CGGGGCTGTTCGACCCGGCGCTGCAGGTCTCGACCCGCCAGGCCTCCTCGATCGGTCTGGGCGGGGAGTTGTTCGCGCGT
CTGCTTGACCAGCATCCGGATGTCGACGGGGTGTTCTTCTGTAACGACGACCTGGCGCAGGGCGCTGCACTTGAGGCCTT
GCGCCTGGGCGTCGCGATTCCCGAACGGGTCTCTCTGGTGGGTTTCAACGACCTGCCGGGGTCCGCGCACATGGTGCCGC
GCCTGACCTCGATACGCACGCCCCGGGAAGAAGTCGGGCAGCGTGCGGCTCAGGTATTGCTGGGCCTGCTTGACGGGATC
TCCCAGCATCCACAGGTGGATCTCGGGTTTGAGCTGGTGGTGCGGGAAAGCTCTTGA

Upstream 100 bases:

>100_bases
CCTGTGAAACCAGCGATAAAGCATTGTCACGTTCCATGAGACAGCGCTACCTTATGGGGCATTCGACACATTTGGCAACC
CCTATGTTAGTTGGCAACGC

Downstream 100 bases:

>100_bases
GTCTTTTTCCCGGATAACCCTGGCGAGCTTCCCCGGTTCATTGCGCTGACCAATGGTAAGGGCGCTCTGCGGGCTCGATG
CCTCCGGGCTGGAGTGCCCG

Product: LacI transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MIRIGSRSTGRPTLNEVAKLAGVSPITASRALRGISTVAPELVEKVRAAAQSLGYVANPAARALASSCSQTVVVLVPSLS
NQLFIETLEAIQDVLRVRGLDVLIGNYHYSPEEEEKLLRNHLVNRPRGILLTGFDHTAASRQMLETSGIPCVHMMELDTR
LGAYCVGFSQQQAGAEAAHHLLGRGRRRLAYMAAQLDPRVLQRGEGFRRVLESAGLFDPALQVSTRQASSIGLGGELFAR
LLDQHPDVDGVFFCNDDLAQGAALEALRLGVAIPERVSLVGFNDLPGSAHMVPRLTSIRTPREEVGQRAAQVLLGLLDGI
SQHPQVDLGFELVVRESS

Sequences:

>Translated_338_residues
MIRIGSRSTGRPTLNEVAKLAGVSPITASRALRGISTVAPELVEKVRAAAQSLGYVANPAARALASSCSQTVVVLVPSLS
NQLFIETLEAIQDVLRVRGLDVLIGNYHYSPEEEEKLLRNHLVNRPRGILLTGFDHTAASRQMLETSGIPCVHMMELDTR
LGAYCVGFSQQQAGAEAAHHLLGRGRRRLAYMAAQLDPRVLQRGEGFRRVLESAGLFDPALQVSTRQASSIGLGGELFAR
LLDQHPDVDGVFFCNDDLAQGAALEALRLGVAIPERVSLVGFNDLPGSAHMVPRLTSIRTPREEVGQRAAQVLLGLLDGI
SQHPQVDLGFELVVRESS
>Mature_338_residues
MIRIGSRSTGRPTLNEVAKLAGVSPITASRALRGISTVAPELVEKVRAAAQSLGYVANPAARALASSCSQTVVVLVPSLS
NQLFIETLEAIQDVLRVRGLDVLIGNYHYSPEEEEKLLRNHLVNRPRGILLTGFDHTAASRQMLETSGIPCVHMMELDTR
LGAYCVGFSQQQAGAEAAHHLLGRGRRRLAYMAAQLDPRVLQRGEGFRRVLESAGLFDPALQVSTRQASSIGLGGELFAR
LLDQHPDVDGVFFCNDDLAQGAALEALRLGVAIPERVSLVGFNDLPGSAHMVPRLTSIRTPREEVGQRAAQVLLGLLDGI
SQHPQVDLGFELVVRESS

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=323, Percent_Identity=37.4613003095975, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790715, Length=324, Percent_Identity=34.5679012345679, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1790369, Length=317, Percent_Identity=29.0220820189274, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=28.2282282282282, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790194, Length=304, Percent_Identity=25.6578947368421, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788474, Length=309, Percent_Identity=27.5080906148867, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787948, Length=321, Percent_Identity=25.8566978193146, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789202, Length=301, Percent_Identity=26.9102990033223, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=25.776397515528, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=26.8518518518519, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=24.8484848484848, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786268, Length=314, Percent_Identity=25.4777070063694, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36474; Mature: 36474

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGSRSTGRPTLNEVAKLAGVSPITASRALRGISTVAPELVEKVRAAAQSLGYVANPA
CEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ARALASSCSQTVVVLVPSLSNQLFIETLEAIQDVLRVRGLDVLIGNYHYSPEEEEKLLRN
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
HLVNRPRGILLTGFDHTAASRQMLETSGIPCVHMMELDTRLGAYCVGFSQQQAGAEAAHH
HHCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLGRGRRRLAYMAAQLDPRVLQRGEGFRRVLESAGLFDPALQVSTRQASSIGLGGELFAR
HHHCHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHH
LLDQHPDVDGVFFCNDDLAQGAALEALRLGVAIPERVSLVGFNDLPGSAHMVPRLTSIRT
HHHHCCCCCEEEEECCHHHCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHCCC
PREEVGQRAAQVLLGLLDGISQHPQVDLGFELVVRESS
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MIRIGSRSTGRPTLNEVAKLAGVSPITASRALRGISTVAPELVEKVRAAAQSLGYVANPA
CEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ARALASSCSQTVVVLVPSLSNQLFIETLEAIQDVLRVRGLDVLIGNYHYSPEEEEKLLRN
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
HLVNRPRGILLTGFDHTAASRQMLETSGIPCVHMMELDTRLGAYCVGFSQQQAGAEAAHH
HHCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLGRGRRRLAYMAAQLDPRVLQRGEGFRRVLESAGLFDPALQVSTRQASSIGLGGELFAR
HHHCHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHH
LLDQHPDVDGVFFCNDDLAQGAALEALRLGVAIPERVSLVGFNDLPGSAHMVPRLTSIRT
HHHHCCCCCEEEEECCHHHCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHCCC
PREEVGQRAAQVLLGLLDGISQHPQVDLGFELVVRESS
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]