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The map label for this gene is yiaJ [H]

Identifier: 66046556

GI number: 66046556

Start: 3979241

End: 3980032

Strand: Direct

Name: yiaJ [H]

Synonym: Psyr_3327

Alternate gene names: 66046556

Gene position: 3979241-3980032 (Clockwise)

Preceding gene: 66046553

Following gene: 66046558

Centisome position: 65.3

GC content: 63.64

Gene sequence:

>792_bases
ATGACCCAGGAAACCGAAAGCCCCGCAGGCGGACGCCAGCAGAAAGTCCAGGCCGCTGAAGTCGGGCTGGGCGTGCTCAA
GGCACTTGCCGAACTCTCCCCTGCCACGTCCCTTTCGAGACTGGCCGAACACCTGGGCATGCCCGCCAGCAAGGTTCATC
GTTATCTGCAGGCGCTGATTGCCAGTGGCTTTGCCGAACAGGATGCTGTCAACAATCACTATGGGCTGGGGCGCGAGGCG
TTGCAGGTGGGGCTGGCCTCGCTCGGCAAGCTGGATGTCCTCAAGGTGTCGGCCCCCTGGCTGGCCAGCTTGCGCGACGA
GTTGAATCAGACCTGCTTTCTGGCGGTATGGGGCAACAAGGGCCCGACCGTGGTGTATGTCGAACCCTCGATGGGTGCGG
TGACGCTGGTCACGCAGATCGGCTCGGTGCTGCCGCTGCTTGGCTCGTCCACCGGCCTGGTGTTCGACAGTTTTCTGAAC
GACCGCGAAACCGCTGCGCTTCGCGAGCAGGAAGTGCCGTTGCTGAGCGCCGCACAGTTGCGTGAGGTCGAGCAGCATGT
CGAGCAGATTCGTACCGCTGGCGTGCACCAGATTCAGGGCTTGCTGATGCCGGGTATCAACGCAGCCTCCTCACCGCTGT
TCGCCATGGGTGACAAGCTGGTGGGCGTGATTACCGTGGTCGGGCCGGGATCGGTGTTGCACGACGCGACACAGGATCTG
GCGGCCACGCGGCTTTTGCAAACGGCGGTGGCGATCAGCGAGCGCATGGGCGGTAGCCGTCCGTCAGACTGA

Upstream 100 bases:

>100_bases
AAACATTATTTTACGTAAAGCGATTACGCATAACGTAATTTGAGTTTGCCAGCCGGTCAAGCTATAAAGCTGCCTTTCCC
GATCGGCGCACAGCACCCCC

Downstream 100 bases:

>100_bases
ATCAGGGGGAAGCGCTGGATACCCTGGCAAAATGGCTATCTGCTTGCCACCCTTGAATCGAGCGACCTTCGACGCGGTTA
CGCCCTTTGGTTTTGGCCAG

Product: regulatory proteins, IclR

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTQETESPAGGRQQKVQAAEVGLGVLKALAELSPATSLSRLAEHLGMPASKVHRYLQALIASGFAEQDAVNNHYGLGREA
LQVGLASLGKLDVLKVSAPWLASLRDELNQTCFLAVWGNKGPTVVYVEPSMGAVTLVTQIGSVLPLLGSSTGLVFDSFLN
DRETAALREQEVPLLSAAQLREVEQHVEQIRTAGVHQIQGLLMPGINAASSPLFAMGDKLVGVITVVGPGSVLHDATQDL
AATRLLQTAVAISERMGGSRPSD

Sequences:

>Translated_263_residues
MTQETESPAGGRQQKVQAAEVGLGVLKALAELSPATSLSRLAEHLGMPASKVHRYLQALIASGFAEQDAVNNHYGLGREA
LQVGLASLGKLDVLKVSAPWLASLRDELNQTCFLAVWGNKGPTVVYVEPSMGAVTLVTQIGSVLPLLGSSTGLVFDSFLN
DRETAALREQEVPLLSAAQLREVEQHVEQIRTAGVHQIQGLLMPGINAASSPLFAMGDKLVGVITVVGPGSVLHDATQDL
AATRLLQTAVAISERMGGSRPSD
>Mature_262_residues
TQETESPAGGRQQKVQAAEVGLGVLKALAELSPATSLSRLAEHLGMPASKVHRYLQALIASGFAEQDAVNNHYGLGREAL
QVGLASLGKLDVLKVSAPWLASLRDELNQTCFLAVWGNKGPTVVYVEPSMGAVTLVTQIGSVLPLLGSSTGLVFDSFLND
RETAALREQEVPLLSAAQLREVEQHVEQIRTAGVHQIQGLLMPGINAASSPLFAMGDKLVGVITVVGPGSVLHDATQDLA
ATRLLQTAVAISERMGGSRPSD

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=270, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082359, Length=263, Percent_Identity=23.9543726235741, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786468, Length=155, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 27661; Mature: 27530

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQETESPAGGRQQKVQAAEVGLGVLKALAELSPATSLSRLAEHLGMPASKVHRYLQALI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
ASGFAEQDAVNNHYGLGREALQVGLASLGKLDVLKVSAPWLASLRDELNQTCFLAVWGNK
HCCCHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCEEEEEEECCC
GPTVVYVEPSMGAVTLVTQIGSVLPLLGSSTGLVFDSFLNDRETAALREQEVPLLSAAQL
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCHHHHHHH
REVEQHVEQIRTAGVHQIQGLLMPGINAASSPLFAMGDKLVGVITVVGPGSVLHDATQDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEEEECCCHHHHHHHHHH
AATRLLQTAVAISERMGGSRPSD
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQETESPAGGRQQKVQAAEVGLGVLKALAELSPATSLSRLAEHLGMPASKVHRYLQALI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
ASGFAEQDAVNNHYGLGREALQVGLASLGKLDVLKVSAPWLASLRDELNQTCFLAVWGNK
HCCCHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCEEEEEEECCC
GPTVVYVEPSMGAVTLVTQIGSVLPLLGSSTGLVFDSFLNDRETAALREQEVPLLSAAQL
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCHHHHHHH
REVEQHVEQIRTAGVHQIQGLLMPGINAASSPLFAMGDKLVGVITVVGPGSVLHDATQDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEEEECCCHHHHHHHHHH
AATRLLQTAVAISERMGGSRPSD
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]