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The map label for this gene is cynX [H]

Identifier: 66046551

GI number: 66046551

Start: 3973247

End: 3974473

Strand: Reverse

Name: cynX [H]

Synonym: Psyr_3322

Alternate gene names: 66046551

Gene position: 3974473-3973247 (Counterclockwise)

Preceding gene: 66046552

Following gene: 66046550

Centisome position: 65.22

GC content: 60.23

Gene sequence:

>1227_bases
ATGCAGATGCGTGGCTCGGCGGTTCAGAATATGGGGTTGATGATCAGTGTCCTGTTGGTGGCGCTGAACCTGCGCCCGTC
GATGGCCGCGGTCGGTCCATTGCTGGTGGCGATTCGTCTGGACGTCCCGCTGGGTTTCGGAACGGCGTCGTTGCTGACCA
TGCTGCCGGTCATGGCCATGGGACTGGCCATGTTTTTCGGCATGGGCATTGCTCGTTACCTGGGTTCGTATCGCAGCATC
ATGCTTTCACTGGCGCTTATCGGTCTGGCAACCCTGGCGCGTCTGTTTCTCGATTCTGCTGCCGAACTGATTTTCAGCGC
TGTGCTGGCGGGTCTGGGTATTGCCTTGATTCAGGCGCTGATGCCCGCGTTGATCAAATCGCGCTTCAAGGCCGACGTCT
CCTTGTACATGGGGCTGTACGTGACCGCGATTATGGGCGGGGCCGCGCTGGCCGCCTCATTCAGCCCTCTGCTAATGAGC
AGCAGTGGCAGTTGGCGCGTCGGCCTGGCGGTCTGTTCGCTACTGGCTCTTCTGGCAATGTGCTTCTGGTTTGCCCAGCG
GCACGTGTTGCAGTCCGACGCGTCAATCGCTGCAGGGCACCGGCAGACCTTCTTCCACATGCCTCGGGCCTGGATGCTGG
GGATTTTCTTCGGTCTTGGCACTGCGTCCTATACCTGTGTGTTGGCCTGGCTGGCGCCCTATTATCTGGAAAACGGCTGG
GGTGAGCAGGGTGCAGGGTTACTGCTGGGCTTTCTGACCCTGATGGAGGTCGTATCGGGTCTGCTGGTTCCGGCGCTGGC
CAACCGCAGTCGCGATAAGCGCGTGGCGCTGGTTGTGCTGCTGATATTGATGATGACCGGCTTTGCCGGGCTGATTTTGA
TGCCGCAGCAGTTGGGCCTGCTGTGGGCATGCCTGCTGGGCTTGGGGATTGGCGGTCTTTTTCCGATGAGCCTGATCGTG
TCCATGGATCATTGCGATGATCCGCAGCAGGCGGGCAGCCTGACGGCATTCGTGCAGGGCGTGGGTTATCTGATCGCCAG
CCTGTCGCCGCTGCTTGCCGGAGTGATTCGTGACCTGACGGGCAGCTTTGCGGGTGCATGGTGGTCACTGATGGCGTTGG
TCGTGATCATGCTGTTGATGGTGGTGCGACTCGACCCTCGACGTTATGCTGATCATCTGCGCGACGCACTCGGCGAACCT
GTCATCACTGGCAGACAGGCTGAATGA

Upstream 100 bases:

>100_bases
CATGGGTGAACTGATTTTTAGATAGCACGGCCCAGATTTTGATTTGTCACCTGAGCACGCGCCCGGCCTAATCATGACCA
TCAATAGCGATGGTGACGGT

Downstream 100 bases:

>100_bases
TTGCATTTTGATGCTATCTTGTGCGCGTTGACCTGACCGCCCGGTCATAGCGCCAGCGAGATGCCTTGATGCTTAACAGT
AATGCCCTGAGAAAGCTGGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; Cyanate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQMRGSAVQNMGLMISVLLVALNLRPSMAAVGPLLVAIRLDVPLGFGTASLLTMLPVMAMGLAMFFGMGIARYLGSYRSI
MLSLALIGLATLARLFLDSAAELIFSAVLAGLGIALIQALMPALIKSRFKADVSLYMGLYVTAIMGGAALAASFSPLLMS
SSGSWRVGLAVCSLLALLAMCFWFAQRHVLQSDASIAAGHRQTFFHMPRAWMLGIFFGLGTASYTCVLAWLAPYYLENGW
GEQGAGLLLGFLTLMEVVSGLLVPALANRSRDKRVALVVLLILMMTGFAGLILMPQQLGLLWACLLGLGIGGLFPMSLIV
SMDHCDDPQQAGSLTAFVQGVGYLIASLSPLLAGVIRDLTGSFAGAWWSLMALVVIMLLMVVRLDPRRYADHLRDALGEP
VITGRQAE

Sequences:

>Translated_408_residues
MQMRGSAVQNMGLMISVLLVALNLRPSMAAVGPLLVAIRLDVPLGFGTASLLTMLPVMAMGLAMFFGMGIARYLGSYRSI
MLSLALIGLATLARLFLDSAAELIFSAVLAGLGIALIQALMPALIKSRFKADVSLYMGLYVTAIMGGAALAASFSPLLMS
SSGSWRVGLAVCSLLALLAMCFWFAQRHVLQSDASIAAGHRQTFFHMPRAWMLGIFFGLGTASYTCVLAWLAPYYLENGW
GEQGAGLLLGFLTLMEVVSGLLVPALANRSRDKRVALVVLLILMMTGFAGLILMPQQLGLLWACLLGLGIGGLFPMSLIV
SMDHCDDPQQAGSLTAFVQGVGYLIASLSPLLAGVIRDLTGSFAGAWWSLMALVVIMLLMVVRLDPRRYADHLRDALGEP
VITGRQAE
>Mature_408_residues
MQMRGSAVQNMGLMISVLLVALNLRPSMAAVGPLLVAIRLDVPLGFGTASLLTMLPVMAMGLAMFFGMGIARYLGSYRSI
MLSLALIGLATLARLFLDSAAELIFSAVLAGLGIALIQALMPALIKSRFKADVSLYMGLYVTAIMGGAALAASFSPLLMS
SSGSWRVGLAVCSLLALLAMCFWFAQRHVLQSDASIAAGHRQTFFHMPRAWMLGIFFGLGTASYTCVLAWLAPYYLENGW
GEQGAGLLLGFLTLMEVVSGLLVPALANRSRDKRVALVVLLILMMTGFAGLILMPQQLGLLWACLLGLGIGGLFPMSLIV
SMDHCDDPQQAGSLTAFVQGVGYLIASLSPLLAGVIRDLTGSFAGAWWSLMALVVIMLLMVVRLDPRRYADHLRDALGEP
VITGRQAE

Specific function: This protein is part of an active transport system that transports exogenous cyanate into E.coli cells [H]

COG id: COG2807

COG function: function code P; Cyanate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Cyanate porter (TC 2.A.1.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786536, Length=382, Percent_Identity=39.2670157068063, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788092, Length=382, Percent_Identity=31.151832460733, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004747
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43495; Mature: 43495

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; Cyanate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + Cyanate [Periplasm] = Proton [Cytoplasm] + Cyanate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3049588; 9278503 [H]