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The map label for this gene is cysG [H]

Identifier: 66046403

GI number: 66046403

Start: 3800897

End: 3802291

Strand: Reverse

Name: cysG [H]

Synonym: Psyr_3174

Alternate gene names: 66046403

Gene position: 3802291-3800897 (Counterclockwise)

Preceding gene: 66046404

Following gene: 66046396

Centisome position: 62.4

GC content: 62.08

Gene sequence:

>1395_bases
ATGGAATTTCTGCCGCTGTTCCATAACCTGCGTGGCAGCCGGGTGCTGGTGGTTGGCGGCGGTGAAATCGCCCTGCGCAA
ATCACGTCTGATTGCCGACGCCGGGGCAGTGCTGCGCGTGGTTGCGCCGGAAATCGAGGCGCAACTGAGCGAGCTGGTTG
TCCAGAGCGGCGGTGAGATGATCCTGCGCGGCTACAGCGAATGCGACCTTGATGGTTGCGTGCTGATTATTGCTGCGACG
GATGATGAGCCGCTCAACGCTCAGGTGTCCCGCGATGCCCGCTTGCGTTGCGTTCCGGTCAACGTCGTCGACGCCCCGGC
GTTGTGTACGGTGATCTTTCCGGCCATCGTCGACCGATCGCCGCTGGTGATCGCCGTGTCCAGTGGCGGCGATGCGCCGG
TGCTGGCACGTTTGATTCGCGCCAAGCTGGAAACCTGGATTCCGTCCTCCTACGGTCAGCTCGCCGGGCTGGCCGCTCGT
TTTCGCAATCAGGTCAAAGGCCTGTTTCCGAACGTGCAGCAGCGTCGGGCGTTTTGGGAAGAAGTTTTTCAGGGGGCCAT
CGCCGATCGCCAACTGGCCGGGCAGGGCGCCGAGGCCGAGCGCATGCTGATCGCCAAGATTGCCGGCGAGCCGCCTCCCG
AGACCGGTGAGGTCTATCTGGTCGGTGCGGGTCCCGGTGATCCGGATTTGCTGACTTTTCGGGCCTTGCGCCTGATGCAG
CAGGCGGATGTGGTGCTGTACGACCGCTTGGTTGCACCGACGATTCTTGACCTGTGCCGTCGTGATGCCGAGCGGGTCTA
CGTTGGCAAGCGCCGTGCGGAACATGCCGTTCCGCAGGAGCAGATCAACCAGCAACTGGTTGCGCTGGCAAAGCAGGGCA
AGCGAGTGGTGCGTCTGAAGGGCGGTGATCCGTTCATATTCGGCCGTGGCGGCGAGGAAATCGAAGAGCTGGCGGCGCAT
GGCATTCCGTTCCAGGTGGTGCCTGGCATCACCGCTGCCAGCGGTTGCGCGGCGTATGCGGGTATACCGCTGACGCACCG
TGATCACGCGCAATCGGTACGTTTTATCACCGGGCACTTGAAAAACGGCACGACCGATCTGCCTTGGTCAGACCTGGTAG
CGCCTGCGCAAACACTGGTGTTTTACATGGGGCTAATCGGTTTGCCAGTCATCTGTGAGCAGTTGATCCGGCACGGTCGC
TCTGCCGACACGCCTGCTGCACTGGTCGAGCAGGGCACCACCGTCAATCAGCGTGTGTTCACGGGTACCCTTGCAAACCT
GCCGCAACTGGTCGCCGAGCATGATGTTCATGCGCCGACGCTGGTGATCATTGGTGAGGTGGTCAAATTGCGTGAAAAGC
TGGCGTGGTTCGAGGGCGCTCAGGCGACTCTCTGA

Upstream 100 bases:

>100_bases
ACTGGTGGCGGTGCTGGAAAACTATCAGCAGGCGGACGGTTCGATTCGTGTGCCTGAGGTGCTCAAGCCTTACATGGGCG
GCCTTGAGGTCATCGGTTAA

Downstream 100 bases:

>100_bases
TGCCAATGGTGGGTAGCCCGTCCGGGCTATCCACCCCTTCTTCCCATACGTCAGTTGTTCCAGACACCTTCACCGGACAG
TCGCTCACGGTCATGATCGG

Product: uroporphyrin-III C-methyltransferase, C-terminal:siroheme synthase, N-terminal

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MEFLPLFHNLRGSRVLVVGGGEIALRKSRLIADAGAVLRVVAPEIEAQLSELVVQSGGEMILRGYSECDLDGCVLIIAAT
DDEPLNAQVSRDARLRCVPVNVVDAPALCTVIFPAIVDRSPLVIAVSSGGDAPVLARLIRAKLETWIPSSYGQLAGLAAR
FRNQVKGLFPNVQQRRAFWEEVFQGAIADRQLAGQGAEAERMLIAKIAGEPPPETGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPTILDLCRRDAERVYVGKRRAEHAVPQEQINQQLVALAKQGKRVVRLKGGDPFIFGRGGEEIEELAAH
GIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFITGHLKNGTTDLPWSDLVAPAQTLVFYMGLIGLPVICEQLIRHGR
SADTPAALVEQGTTVNQRVFTGTLANLPQLVAEHDVHAPTLVIIGEVVKLREKLAWFEGAQATL

Sequences:

>Translated_464_residues
MEFLPLFHNLRGSRVLVVGGGEIALRKSRLIADAGAVLRVVAPEIEAQLSELVVQSGGEMILRGYSECDLDGCVLIIAAT
DDEPLNAQVSRDARLRCVPVNVVDAPALCTVIFPAIVDRSPLVIAVSSGGDAPVLARLIRAKLETWIPSSYGQLAGLAAR
FRNQVKGLFPNVQQRRAFWEEVFQGAIADRQLAGQGAEAERMLIAKIAGEPPPETGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPTILDLCRRDAERVYVGKRRAEHAVPQEQINQQLVALAKQGKRVVRLKGGDPFIFGRGGEEIEELAAH
GIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFITGHLKNGTTDLPWSDLVAPAQTLVFYMGLIGLPVICEQLIRHGR
SADTPAALVEQGTTVNQRVFTGTLANLPQLVAEHDVHAPTLVIIGEVVKLREKLAWFEGAQATL
>Mature_464_residues
MEFLPLFHNLRGSRVLVVGGGEIALRKSRLIADAGAVLRVVAPEIEAQLSELVVQSGGEMILRGYSECDLDGCVLIIAAT
DDEPLNAQVSRDARLRCVPVNVVDAPALCTVIFPAIVDRSPLVIAVSSGGDAPVLARLIRAKLETWIPSSYGQLAGLAAR
FRNQVKGLFPNVQQRRAFWEEVFQGAIADRQLAGQGAEAERMLIAKIAGEPPPETGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPTILDLCRRDAERVYVGKRRAEHAVPQEQINQQLVALAKQGKRVVRLKGGDPFIFGRGGEEIEELAAH
GIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFITGHLKNGTTDLPWSDLVAPAQTLVFYMGLIGLPVICEQLIRHGR
SADTPAALVEQGTTVNQRVFTGTLANLPQLVAEHDVHAPTLVIIGEVVKLREKLAWFEGAQATL

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=458, Percent_Identity=53.9301310043668, Blast_Score=460, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322922, Length=244, Percent_Identity=38.1147540983607, Blast_Score=146, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50047; Mature: 50047

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLPLFHNLRGSRVLVVGGGEIALRKSRLIADAGAVLRVVAPEIEAQLSELVVQSGGEM
CCCCCHHHCCCCCEEEEEECCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCE
ILRGYSECDLDGCVLIIAATDDEPLNAQVSRDARLRCVPVNVVDAPALCTVIFPAIVDRS
EEECCCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
PLVIAVSSGGDAPVLARLIRAKLETWIPSSYGQLAGLAARFRNQVKGLFPNVQQRRAFWE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EVFQGAIADRQLAGQGAEAERMLIAKIAGEPPPETGEVYLVGAGPGDPDLLTFRALRLMQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
QADVVLYDRLVAPTILDLCRRDAERVYVGKRRAEHAVPQEQINQQLVALAKQGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEEIEELAAHGIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFITGHL
CCCEEEECCCCHHHHHHHHCCCCEEECCCCCHHCCCHHHCCCCCCCCHHHHHHHHHEEEC
KNGTTDLPWSDLVAPAQTLVFYMGLIGLPVICEQLIRHGRSADTPAALVEQGTTVNQRVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHH
TGTLANLPQLVAEHDVHAPTLVIIGEVVKLREKLAWFEGAQATL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEFLPLFHNLRGSRVLVVGGGEIALRKSRLIADAGAVLRVVAPEIEAQLSELVVQSGGEM
CCCCCHHHCCCCCEEEEEECCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCE
ILRGYSECDLDGCVLIIAATDDEPLNAQVSRDARLRCVPVNVVDAPALCTVIFPAIVDRS
EEECCCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
PLVIAVSSGGDAPVLARLIRAKLETWIPSSYGQLAGLAARFRNQVKGLFPNVQQRRAFWE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EVFQGAIADRQLAGQGAEAERMLIAKIAGEPPPETGEVYLVGAGPGDPDLLTFRALRLMQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
QADVVLYDRLVAPTILDLCRRDAERVYVGKRRAEHAVPQEQINQQLVALAKQGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEEIEELAAHGIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFITGHL
CCCEEEECCCCHHHHHHHHCCCCEEECCCCCHHCCCHHHCCCCCCCCHHHHHHHHHEEEC
KNGTTDLPWSDLVAPAQTLVFYMGLIGLPVICEQLIRHGRSADTPAALVEQGTTVNQRVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHH
TGTLANLPQLVAEHDVHAPTLVIIGEVVKLREKLAWFEGAQATL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA