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The map label for this gene is citH [H]

Identifier: 66046396

GI number: 66046396

Start: 3795830

End: 3797131

Strand: Reverse

Name: citH [H]

Synonym: Psyr_3167

Alternate gene names: 66046396

Gene position: 3797131-3795830 (Counterclockwise)

Preceding gene: 66046403

Following gene: 66046395

Centisome position: 62.31

GC content: 59.45

Gene sequence:

>1302_bases
ATGCACGGCTCATCCTCATTACGAAAAGAATCCTCCCGGTCGAAGGCAGGCGCGGTATTTCGGGTCACCTCCGGTAATTT
CCTCGAGCAATTCGACTTCTTCCTGTTCGGCTTCTATGCCACCCAGATCGCTTCCGCCTTTTTCCCGGCCAGCAGTGAGT
TCGCTTCGCTGATGATGACCTTCGCGGTCTTCGGCGCGGGCTTCCTGATGCGTCCGCTGGGTGCCGTCATTCTCGGTGCC
TACATCGATGACGTGGGCCGCCGCAAAGGGCTGATCGTGACGCTTTCGATCATGGCCAGTGGCACGTTACTGATTGTTCT
GGTGCCCGGTTACGCAAGCATCGGCCTGTGGGCGCCGGCCTTGGTATTGGTCGGCCGCTTGCTGCAAGGCTTCTCTGCCG
GTGCGGAATTGGGCGGGGTGTCGGTGTATCTGGCAGAAATGGCGACACCCGGCCGCAAGGGCTTCTACACCAGTTGGCAG
TCAGGTAGTCAGCAGGTGGCAATCGTTGTCGCCGCTGCACTGGGTTACGGCCTGAACCAATGGATGGCACCTGCTGCTGT
TGCCGACTGGGGCTGGAGAATTCCGTTTGCCATCGGTTGCCTGATCATCCCGTTCATTTTCCTGTTGCGCCGCAGCCTGC
AGGAAACCGAAGAGTTCGCTACTCGCTCTCACCGTCCGACCATGCGACAGGTGTTCGCGACGCTGCTGCAAAACTGGCGT
GTGGTGATTGCCGGAATGCTGATGGTGGCGATGACCACTACCACCTTTTACCTGATCACCATCTATGCACCGACATTCGG
CAAAACCGTACTCAACCTGAGTACTGCCGATGCGCTTCTGGTGACGCTGCTGGTCGGTGTCTCCAACTTTATCTGGCTGC
CGGTCGGCGGCATGTTGAGTGACCGTTTCGGGCGCAAGCCACTGTTGGTCGCGATGACCCTGCTGACCATCATCAGTGCG
TACCCGGCGCTGTCTTTCCTCGCCCTGGCGCCCAGCTTCGGCCATATGCTGGAAGTGCTGTTGTGGTTCTCGTTCCTCTA
CGGCTTGTATAACGGCGCGATGATTCCGGCGCTGACCGAAATCATGCCGGTAGAGGTGCGTGTTGCCGGTTTCTCGCTGG
CCTACAGCCTGGCGACGGCGGTTTTCGGTGGTTTCACTCCGGCGATCTCGACCTGGCTGATCCACCTGACCGGCGACAAG
GCTGCACCTGCTTACTGGATGACATTTGCTGCAGCCTGTGCGCTGGGCGCCACACTGGTGCTCTACCGGCACATCTCGAC
CCGCCCGCAACTGGCTGTTTAA

Upstream 100 bases:

>100_bases
AATCGATGAAAACATAGCACTTATCGTTTCAAAGTGTGTCCGGCAGACTCCTTCCTCACAAGCGCTTACAACACTCTAAT
AAGATCGCTGGAGAACACTG

Downstream 100 bases:

>100_bases
TCAAGGAAAAATAACTATGAAATCGTTATACAAACATCAACTGGCAGCGCTTTTTCTCGGCTGTATCGCGGTCAGTGCGG
CGGCGCAGGCTGAACAGCTC

Product: citrate-proton symport

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MHGSSSLRKESSRSKAGAVFRVTSGNFLEQFDFFLFGFYATQIASAFFPASSEFASLMMTFAVFGAGFLMRPLGAVILGA
YIDDVGRRKGLIVTLSIMASGTLLIVLVPGYASIGLWAPALVLVGRLLQGFSAGAELGGVSVYLAEMATPGRKGFYTSWQ
SGSQQVAIVVAAALGYGLNQWMAPAAVADWGWRIPFAIGCLIIPFIFLLRRSLQETEEFATRSHRPTMRQVFATLLQNWR
VVIAGMLMVAMTTTTFYLITIYAPTFGKTVLNLSTADALLVTLLVGVSNFIWLPVGGMLSDRFGRKPLLVAMTLLTIISA
YPALSFLALAPSFGHMLEVLLWFSFLYGLYNGAMIPALTEIMPVEVRVAGFSLAYSLATAVFGGFTPAISTWLIHLTGDK
AAPAYWMTFAAACALGATLVLYRHISTRPQLAV

Sequences:

>Translated_433_residues
MHGSSSLRKESSRSKAGAVFRVTSGNFLEQFDFFLFGFYATQIASAFFPASSEFASLMMTFAVFGAGFLMRPLGAVILGA
YIDDVGRRKGLIVTLSIMASGTLLIVLVPGYASIGLWAPALVLVGRLLQGFSAGAELGGVSVYLAEMATPGRKGFYTSWQ
SGSQQVAIVVAAALGYGLNQWMAPAAVADWGWRIPFAIGCLIIPFIFLLRRSLQETEEFATRSHRPTMRQVFATLLQNWR
VVIAGMLMVAMTTTTFYLITIYAPTFGKTVLNLSTADALLVTLLVGVSNFIWLPVGGMLSDRFGRKPLLVAMTLLTIISA
YPALSFLALAPSFGHMLEVLLWFSFLYGLYNGAMIPALTEIMPVEVRVAGFSLAYSLATAVFGGFTPAISTWLIHLTGDK
AAPAYWMTFAAACALGATLVLYRHISTRPQLAV
>Mature_433_residues
MHGSSSLRKESSRSKAGAVFRVTSGNFLEQFDFFLFGFYATQIASAFFPASSEFASLMMTFAVFGAGFLMRPLGAVILGA
YIDDVGRRKGLIVTLSIMASGTLLIVLVPGYASIGLWAPALVLVGRLLQGFSAGAELGGVSVYLAEMATPGRKGFYTSWQ
SGSQQVAIVVAAALGYGLNQWMAPAAVADWGWRIPFAIGCLIIPFIFLLRRSLQETEEFATRSHRPTMRQVFATLLQNWR
VVIAGMLMVAMTTTTFYLITIYAPTFGKTVLNLSTADALLVTLLVGVSNFIWLPVGGMLSDRFGRKPLLVAMTLLTIISA
YPALSFLALAPSFGHMLEVLLWFSFLYGLYNGAMIPALTEIMPVEVRVAGFSLAYSLATAVFGGFTPAISTWLIHLTGDK
AAPAYWMTFAAACALGATLVLYRHISTRPQLAV

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=418, Percent_Identity=30.3827751196172, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=33.8785046728972, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788292, Length=430, Percent_Identity=29.0697674418605, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1789941, Length=382, Percent_Identity=29.8429319371728, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082231, Length=233, Percent_Identity=26.6094420600858, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46759; Mature: 46759

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGSSSLRKESSRSKAGAVFRVTSGNFLEQFDFFLFGFYATQIASAFFPASSEFASLMMT
CCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FAVFGAGFLMRPLGAVILGAYIDDVGRRKGLIVTLSIMASGTLLIVLVPGYASIGLWAPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHH
LVLVGRLLQGFSAGAELGGVSVYLAEMATPGRKGFYTSWQSGSQQVAIVVAAALGYGLNQ
HHHHHHHHHHHCCCCCCCCHHEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
WMAPAAVADWGWRIPFAIGCLIIPFIFLLRRSLQETEEFATRSHRPTMRQVFATLLQNWR
HHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVIAGMLMVAMTTTTFYLITIYAPTFGKTVLNLSTADALLVTLLVGVSNFIWLPVGGMLS
HHHHHHHHHHHHHHHEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHEEEECCCHHHH
DRFGRKPLLVAMTLLTIISAYPALSFLALAPSFGHMLEVLLWFSFLYGLYNGAMIPALTE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IMPVEVRVAGFSLAYSLATAVFGGFTPAISTWLIHLTGDKAAPAYWMTFAAACALGATLV
HCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LYRHISTRPQLAV
HHHHHCCCCCCCC
>Mature Secondary Structure
MHGSSSLRKESSRSKAGAVFRVTSGNFLEQFDFFLFGFYATQIASAFFPASSEFASLMMT
CCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FAVFGAGFLMRPLGAVILGAYIDDVGRRKGLIVTLSIMASGTLLIVLVPGYASIGLWAPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHH
LVLVGRLLQGFSAGAELGGVSVYLAEMATPGRKGFYTSWQSGSQQVAIVVAAALGYGLNQ
HHHHHHHHHHHCCCCCCCCHHEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
WMAPAAVADWGWRIPFAIGCLIIPFIFLLRRSLQETEEFATRSHRPTMRQVFATLLQNWR
HHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVIAGMLMVAMTTTTFYLITIYAPTFGKTVLNLSTADALLVTLLVGVSNFIWLPVGGMLS
HHHHHHHHHHHHHHHEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHEEEECCCHHHH
DRFGRKPLLVAMTLLTIISAYPALSFLALAPSFGHMLEVLLWFSFLYGLYNGAMIPALTE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IMPVEVRVAGFSLAYSLATAVFGGFTPAISTWLIHLTGDKAAPAYWMTFAAACALGATLV
HCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LYRHISTRPQLAV
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]