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The map label for this gene is yafC [H]

Identifier: 66046293

GI number: 66046293

Start: 3661504

End: 3662400

Strand: Reverse

Name: yafC [H]

Synonym: Psyr_3062

Alternate gene names: 66046293

Gene position: 3662400-3661504 (Counterclockwise)

Preceding gene: 66046297

Following gene: 66046292

Centisome position: 60.1

GC content: 57.86

Gene sequence:

>897_bases
TTGGAATCGCTCGGAAGCATCGCCGTGTTCGTTCTGGTTGCTGAAACGCGGAGTTTTACCCAGGCCGGAAAACGCCTGGG
CATCTCTTCGTCGGGGATCGGCAAGAGTATTGGCCGTCTGGAGGAGAGCCTCGGCGTCCGCCTTTTTCATCGCAGTACGC
GCAGTATCGCCCTGACGCTGGAAGGCTCGATATTCCTTGAGCGTTGCCGGCGGATTCTGTCGGAGCTGGAAGCGGCGGAA
ATGGAGTTGAGCAATGCCAGAGACTTGCCCAAGGGCAGGTTGCGGATCAGTTTGCCATTGGTCAGCGGACTGGTGATGCC
GGTGATCATCGACTTCATGCGTACCTATCCCGATATCGAACTGGACATCGATTTCTCCGACCGCATGGTCGATATCATCG
AGGAGGGCTTCGATATCGTGTTACGCACCGGCGATCCCGGTGACTCGGGGCTGATGTCGAAGCGGATGGGCAGTTTCCAA
TTGCGCGTTGTCGGCGCTCCGGACTATTTTGCCCGGCACGGTATACCCCGGCATCCTGGGGACCTGAGTCAGCACGCCTG
CCTGTTGCACAAGTTTCCTTCCACTGGAAGGTTTGAACACTGGCCGCTGCGCCGTGAGGCGGGCGTCGACGATCTCGAGT
TACCCCAGGCCATGGTGTGTAATACCACCGAGATTCTGGTGGACGTCGCGCGGGCCGGGTTGGGAGTTGCCTGTCTGCCG
GACTTCATGATTTTATCGGCCATTCGCAACGGCGAGCTGATACCTGTCCTGGATGACTACACGGTGCATACCGGAACGTT
CAGATTGCTCTGGCCGTCCAGCAAGCATCTTTCGCTGCGCCTGCGAGTCTTCATTGATTTCATGCACACCCATTTGTTCG
CTCCTCCGTCAGTATGA

Upstream 100 bases:

>100_bases
AGCGATCGCCCTGCCGCGAGATTAGGGGTTGCGCCCGCAAACAAAAACAGGCTTATGTTCCGAACGTATAGGACAGGGTT
GTCCTGAATAGGAGTGGGTA

Downstream 100 bases:

>100_bases
CTGCGCGGAGCGAGCCTGCAGCACCTGGCAAGGAGCACCGCTATGTACACGCTTTTCGGCACGCAAGGTTCGGGTTCGGC
TGCGATCGAGATAGCGCTTC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MESLGSIAVFVLVAETRSFTQAGKRLGISSSGIGKSIGRLEESLGVRLFHRSTRSIALTLEGSIFLERCRRILSELEAAE
MELSNARDLPKGRLRISLPLVSGLVMPVIIDFMRTYPDIELDIDFSDRMVDIIEEGFDIVLRTGDPGDSGLMSKRMGSFQ
LRVVGAPDYFARHGIPRHPGDLSQHACLLHKFPSTGRFEHWPLRREAGVDDLELPQAMVCNTTEILVDVARAGLGVACLP
DFMILSAIRNGELIPVLDDYTVHTGTFRLLWPSSKHLSLRLRVFIDFMHTHLFAPPSV

Sequences:

>Translated_298_residues
MESLGSIAVFVLVAETRSFTQAGKRLGISSSGIGKSIGRLEESLGVRLFHRSTRSIALTLEGSIFLERCRRILSELEAAE
MELSNARDLPKGRLRISLPLVSGLVMPVIIDFMRTYPDIELDIDFSDRMVDIIEEGFDIVLRTGDPGDSGLMSKRMGSFQ
LRVVGAPDYFARHGIPRHPGDLSQHACLLHKFPSTGRFEHWPLRREAGVDDLELPQAMVCNTTEILVDVARAGLGVACLP
DFMILSAIRNGELIPVLDDYTVHTGTFRLLWPSSKHLSLRLRVFIDFMHTHLFAPPSV
>Mature_298_residues
MESLGSIAVFVLVAETRSFTQAGKRLGISSSGIGKSIGRLEESLGVRLFHRSTRSIALTLEGSIFLERCRRILSELEAAE
MELSNARDLPKGRLRISLPLVSGLVMPVIIDFMRTYPDIELDIDFSDRMVDIIEEGFDIVLRTGDPGDSGLMSKRMGSFQ
LRVVGAPDYFARHGIPRHPGDLSQHACLLHKFPSTGRFEHWPLRREAGVDDLELPQAMVCNTTEILVDVARAGLGVACLP
DFMILSAIRNGELIPVLDDYTVHTGTFRLLWPSSKHLSLRLRVFIDFMHTHLFAPPSV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=34.4709897610922, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=33.3333333333333, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=31.3793103448276, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=32.8719723183391, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=30.2721088435374, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=30.5841924398625, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=24.4755244755245, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789173, Length=274, Percent_Identity=28.1021897810219, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790011, Length=262, Percent_Identity=22.5190839694656, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=26.2798634812287, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788706, Length=264, Percent_Identity=25, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=120, Percent_Identity=39.1666666666667, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790262, Length=243, Percent_Identity=25.1028806584362, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790783, Length=263, Percent_Identity=28.5171102661597, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786713, Length=270, Percent_Identity=28.5185185185185, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787879, Length=139, Percent_Identity=33.8129496402878, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788748, Length=155, Percent_Identity=34.1935483870968, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=29.5081967213115, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788481, Length=301, Percent_Identity=27.2425249169435, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=142, Percent_Identity=30.9859154929577, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33165; Mature: 33165

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLGSIAVFVLVAETRSFTQAGKRLGISSSGIGKSIGRLEESLGVRLFHRSTRSIALTL
CCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEEECCCCEEEEEE
EGSIFLERCRRILSELEAAEMELSNARDLPKGRLRISLPLVSGLVMPVIIDFMRTYPDIE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
LDIDFSDRMVDIIEEGFDIVLRTGDPGDSGLMSKRMGSFQLRVVGAPDYFARHGIPRHPG
EEECCHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCEEEEEEECCCHHHHCCCCCCCC
DLSQHACLLHKFPSTGRFEHWPLRREAGVDDLELPQAMVCNTTEILVDVARAGLGVACLP
CHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHCCCHHHHH
DFMILSAIRNGELIPVLDDYTVHTGTFRLLWPSSKHLSLRLRVFIDFMHTHLFAPPSV
HHHHHHHHCCCCEEEEECCCEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MESLGSIAVFVLVAETRSFTQAGKRLGISSSGIGKSIGRLEESLGVRLFHRSTRSIALTL
CCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEEECCCCEEEEEE
EGSIFLERCRRILSELEAAEMELSNARDLPKGRLRISLPLVSGLVMPVIIDFMRTYPDIE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
LDIDFSDRMVDIIEEGFDIVLRTGDPGDSGLMSKRMGSFQLRVVGAPDYFARHGIPRHPG
EEECCHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCEEEEEEECCCHHHHCCCCCCCC
DLSQHACLLHKFPSTGRFEHWPLRREAGVDDLELPQAMVCNTTEILVDVARAGLGVACLP
CHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHCCCHHHHH
DFMILSAIRNGELIPVLDDYTVHTGTFRLLWPSSKHLSLRLRVFIDFMHTHLFAPPSV
HHHHHHHHCCCCEEEEECCCEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]