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The map label for this gene is gloA [H]

Identifier: 66046204

GI number: 66046204

Start: 3565492

End: 3566013

Strand: Direct

Name: gloA [H]

Synonym: Psyr_2973

Alternate gene names: 66046204

Gene position: 3565492-3566013 (Clockwise)

Preceding gene: 66046202

Following gene: 66046208

Centisome position: 58.51

GC content: 59.0

Gene sequence:

>522_bases
ATGAGCTTGCACGAACTCAACACCCTACCGGGCGTCACAGCCAATCCTGAAGCGGCCACCCGGCAGTTTGTGTTCAACCA
CACCATGCTGCGCGTCAAGGACATCACCAAATCGCTGGACTTCTACACCCGTGTACTGGGCTTCAGCCTGGTCGAAAAAC
GAGACTTCCCGGAAGCCGAATTCAGCCTGTACTTCCTGGCGCTGGTCGACAAGGCGCAGATCCCGGAAGACGACGCTGCG
CGCAACGAATGGATGAAGTCGATCGCCGGCATTCTGGAGCTGACCCATAACCACGGCACCGAAAGCGATGACACAGCGTC
GTACCACAACGGCAACAGCGACCCGCGCGGCTTTGGCCATATCTGCGTGTCGGTGCCTGACGTGAAAGTCGCCTGCGAGC
GATTCGAAAGCCTCGGCGTCGACTTCCAGAAGCGCTTGTCCGACGGCCGCATGAACAGCCTGGCCTTTATCAAGGACCCG
GATGGCTACTGGGTAGAGATCATCCAGCCTACACCGCTCTGA

Upstream 100 bases:

>100_bases
CGAGCAAGGCCGACGCGACGTTATCCAAGCCGGGTTTGGCACTGCTCGCCCTGCCCCGTATCATTTGGCATTATTTGTAC
ATTCTGTTGGAGACACATTG

Downstream 100 bases:

>100_bases
GACTGGCGGTGATCGCTCCGGCGAGGAGCGATCAGCCTGAAAAACAAAAAGCCCCGTGATCGCTCACGGGGCTTTTTGTT
TACCGCTGTCCGATCAAGCC

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSLHELNTLPGVTANPEAATRQFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAEFSLYFLALVDKAQIPEDDAA
RNEWMKSIAGILELTHNHGTESDDTASYHNGNSDPRGFGHICVSVPDVKVACERFESLGVDFQKRLSDGRMNSLAFIKDP
DGYWVEIIQPTPL

Sequences:

>Translated_173_residues
MSLHELNTLPGVTANPEAATRQFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAEFSLYFLALVDKAQIPEDDAA
RNEWMKSIAGILELTHNHGTESDDTASYHNGNSDPRGFGHICVSVPDVKVACERFESLGVDFQKRLSDGRMNSLAFIKDP
DGYWVEIIQPTPL
>Mature_172_residues
SLHELNTLPGVTANPEAATRQFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAEFSLYFLALVDKAQIPEDDAAR
NEWMKSIAGILELTHNHGTESDDTASYHNGNSDPRGFGHICVSVPDVKVACERFESLGVDFQKRLSDGRMNSLAFIKDPD
GYWVEIIQPTPL

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=162, Percent_Identity=58.0246913580247, Blast_Score=204, Evalue=4e-53,
Organism=Escherichia coli, GI1787940, Length=145, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323639, Length=151, Percent_Identity=49.0066225165563, Blast_Score=147, Evalue=8e-37,
Organism=Drosophila melanogaster, GI20129743, Length=157, Percent_Identity=56.0509554140127, Blast_Score=193, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 19492; Mature: 19361

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHELNTLPGVTANPEAATRQFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FSLYFLALVDKAQIPEDDAARNEWMKSIAGILELTHNHGTESDDTASYHNGNSDPRGFGH
HHHHHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
ICVSVPDVKVACERFESLGVDFQKRLSDGRMNSLAFIKDPDGYWVEIIQPTPL
EEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCEEEEECCCCC
>Mature Secondary Structure 
SLHELNTLPGVTANPEAATRQFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FSLYFLALVDKAQIPEDDAARNEWMKSIAGILELTHNHGTESDDTASYHNGNSDPRGFGH
HHHHHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
ICVSVPDVKVACERFESLGVDFQKRLSDGRMNSLAFIKDPDGYWVEIIQPTPL
EEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959071 [H]