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The map label for this gene is xylF [H]

Identifier: 66046115

GI number: 66046115

Start: 3460307

End: 3461308

Strand: Direct

Name: xylF [H]

Synonym: Psyr_2884

Alternate gene names: 66046115

Gene position: 3460307-3461308 (Clockwise)

Preceding gene: 66046114

Following gene: 66046116

Centisome position: 56.79

GC content: 57.49

Gene sequence:

>1002_bases
ATGAACACCCTCAAAAGTACGCTGCTCGCCAGCGCGCTGGCCTTGCTGTCCCTGCCGGTGATGGCTGACTCTGCGCACCC
GAAAATAGGTTTTTCCATTGATGACCTGCGTCTGGAACGCTGGTCACGCGACCGTGATTACTTCGTTGCGGCGGCGGAAA
AGCTCGACGCCAAGGTCTTCGTGCAATCAGCGGATGCCAATGAACAGAAACAGATCTCGCAGATAGAAAACCTCATCTCT
CGCGGCGTCGATGTGATCGTCATCGTGCCGTTCAACGCCACCGTGCTGACCAACGCCGTTGCCGAGGCCAAGAAGGCCGG
GATCAAGGTGGTGTCGTATGACCGGCTGATCCTCAATGCCGATATCGACGCGTACATTTCCTTCGATAACGAAAAAGTCG
GCGAGATGCAGGCCAACGGCGTGCTGAAAGCAGCGCCCAAGGGCAATTACTTCCTGCTGGGCGGCGCGCCCACCGACAAC
AACGCCAAGATTCTGCGCGAAGGCCAGATGAAGGCGCTGCAACCGGCTATCGACAGAGGTGATATCAAGATCGTCGGCCA
GCAGTGGGTAAAGGAGTGGAACCCTACCGAAGCCCTGAGCATTGTTGAAAACGCCCTGACGCGTAATAACAACAAGATCG
ATGGCATCGTCGCCTCCAACGACGCCACTGCCGGCGGCGCGATTCAGGCCCTCGCCGCACAGAAACTGGCCGGCAAAGTC
CCTATCTCGGGTCAGGACGCGGACCTCGCGGCGGTCAAGCGGGTCATCGATGGCACGCAGACCATGACTGTCTACAAGCC
GCTGAAACTGATTGCCACGGAAGCCGCCAAGCTCTCTGTGCAACTGGCACGCAATGAGAAACCCGATTTCAGCTCGAAAT
ACGACAACGGCAGCAAACAAGTCGACACCATCCTGCTGACGCCTACAGCGTTGACCAAGGACAACATCGACCTGCTGGAG
AAAGACGGGTTCTATACCAAGGCGCAGATTGACGGGCAATGA

Upstream 100 bases:

>100_bases
CGACAAATCTTCATACGCGACGTCCGGCAACGCTCTACAGTTCTATCGGTATCACCCACAGTCAAGCAGTGTCTCAAACA
ACAATAAAAGGACGCACCCT

Downstream 100 bases:

>100_bases
GCCTCGATCGTTGATACGTCGCTGAAAGCCAGAGCACGACCCTGCGGTTTAGCCGGGTCGCCGCTCAGGGCAAGCCTTCA
CACCTTCGTAGAGCCCTGCG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNTLKSTLLASALALLSLPVMADSAHPKIGFSIDDLRLERWSRDRDYFVAAAEKLDAKVFVQSADANEQKQISQIENLIS
RGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNADIDAYISFDNEKVGEMQANGVLKAAPKGNYFLLGGAPTDN
NAKILREGQMKALQPAIDRGDIKIVGQQWVKEWNPTEALSIVENALTRNNNKIDGIVASNDATAGGAIQALAAQKLAGKV
PISGQDADLAAVKRVIDGTQTMTVYKPLKLIATEAAKLSVQLARNEKPDFSSKYDNGSKQVDTILLTPTALTKDNIDLLE
KDGFYTKAQIDGQ

Sequences:

>Translated_333_residues
MNTLKSTLLASALALLSLPVMADSAHPKIGFSIDDLRLERWSRDRDYFVAAAEKLDAKVFVQSADANEQKQISQIENLIS
RGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNADIDAYISFDNEKVGEMQANGVLKAAPKGNYFLLGGAPTDN
NAKILREGQMKALQPAIDRGDIKIVGQQWVKEWNPTEALSIVENALTRNNNKIDGIVASNDATAGGAIQALAAQKLAGKV
PISGQDADLAAVKRVIDGTQTMTVYKPLKLIATEAAKLSVQLARNEKPDFSSKYDNGSKQVDTILLTPTALTKDNIDLLE
KDGFYTKAQIDGQ
>Mature_333_residues
MNTLKSTLLASALALLSLPVMADSAHPKIGFSIDDLRLERWSRDRDYFVAAAEKLDAKVFVQSADANEQKQISQIENLIS
RGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNADIDAYISFDNEKVGEMQANGVLKAAPKGNYFLLGGAPTDN
NAKILREGQMKALQPAIDRGDIKIVGQQWVKEWNPTEALSIVENALTRNNNKIDGIVASNDATAGGAIQALAAQKLAGKV
PISGQDADLAAVKRVIDGTQTMTVYKPLKLIATEAAKLSVQLARNEKPDFSSKYDNGSKQVDTILLTPTALTKDNIDLLE
KDGFYTKAQIDGQ

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=312, Percent_Identity=61.2179487179487, Blast_Score=393, Evalue=1e-111,
Organism=Escherichia coli, GI1790526, Length=265, Percent_Identity=28.6792452830189, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790192, Length=298, Percent_Identity=26.1744966442953, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35937; Mature: 35937

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLKSTLLASALALLSLPVMADSAHPKIGFSIDDLRLERWSRDRDYFVAAAEKLDAKVF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHCCEEEE
VQSADANEQKQISQIENLISRGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNA
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEECEEEEEC
DIDAYISFDNEKVGEMQANGVLKAAPKGNYFLLGGAPTDNNAKILREGQMKALQPAIDRG
CCEEEEEECCCCCCCHHCCCEEEECCCCCEEEEECCCCCCCCCEEECCCHHHHHHCCCCC
DIKIVGQQWVKEWNPTEALSIVENALTRNNNKIDGIVASNDATAGGAIQALAAQKLAGKV
CEEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
PISGQDADLAAVKRVIDGTQTMTVYKPLKLIATEAAKLSVQLARNEKPDFSSKYDNGSKQ
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCE
VDTILLTPTALTKDNIDLLEKDGFYTKAQIDGQ
EEEEEECCCCCCCCCCHHEECCCCEEEEEECCC
>Mature Secondary Structure
MNTLKSTLLASALALLSLPVMADSAHPKIGFSIDDLRLERWSRDRDYFVAAAEKLDAKVF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHCCEEEE
VQSADANEQKQISQIENLISRGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNA
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEECEEEEEC
DIDAYISFDNEKVGEMQANGVLKAAPKGNYFLLGGAPTDNNAKILREGQMKALQPAIDRG
CCEEEEEECCCCCCCHHCCCEEEECCCCCEEEEECCCCCCCCCEEECCCHHHHHHCCCCC
DIKIVGQQWVKEWNPTEALSIVENALTRNNNKIDGIVASNDATAGGAIQALAAQKLAGKV
CEEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
PISGQDADLAAVKRVIDGTQTMTVYKPLKLIATEAAKLSVQLARNEKPDFSSKYDNGSKQ
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCE
VDTILLTPTALTKDNIDLLEKDGFYTKAQIDGQ
EEEEEECCCCCCCCCCHHEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]