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The map label for this gene is paaF [C]

Identifier: 66045964

GI number: 66045964

Start: 3311551

End: 3312381

Strand: Reverse

Name: paaF [C]

Synonym: Psyr_2728

Alternate gene names: 66045964

Gene position: 3312381-3311551 (Counterclockwise)

Preceding gene: 66045966

Following gene: 66045963

Centisome position: 54.36

GC content: 58.84

Gene sequence:

>831_bases
ATGAGCAAATATGAAGGTCGCTGGTCAACCATCAAGGTCGAGATCGAGCAAGGTATTGCGTGGGTCATCCTCAATCGGCC
GGAAAAGCGCAACGCCATGAGCCCGACCCTGAACCGCGAAATGATCGATGTGCTGGAAACCCTCGAGCAGGATCCCGATG
CGGGGGTTCTGGTGCTGACCGGTGCGGGTGAGGCGTGGACCGCTGGCATGGACCTCAAGGAGTACTTCCGCGAGGTGGAT
GCCGGACCGGAAATTCTTCAGGAAAAAATCCGGCGCGAAGCCTCCCAGTGGCAGTGGAAGATGTTACGCATGTACGCCAA
GCCAACCATCGCAATGGTCAACGGCTGGTGCTTTGGTGGTGGTTTCAGCCCGCTGGTGGCCTGTGATCTGGCGATCTGCG
CCGATGAAGCGACCTTCGGCCTGTCGGAAATCAACTGGGGCATTCCGCCCGGCAACCTGGTGAGCAAGGCCATGGCCGAC
ACCGTGGGCCACCGCCAGTCGCTGTACTACATCATGACCGGCAAAACCTTCAATGGTCAGAAGGCCGCTGAAATGGGCCT
CGTCAATGAAAGCGTACCGCTGGCGCAACTGCGTCAGGTGGCCATCGATCTGGCCAATAATCTGCTGGAAAAAAATCCGG
TGGTGCTGCGCGCCGCCAAGCACGGCTTCAAACGCTGCCGCGAACTGACCTGGGAGCAGAACGAAGACTACCTGTACGCC
AAGCTCGACCAGTCACGTCTGCTGGACAAGGAAGGCGGGCGCGAGCAGGGCATGAAGCAGTTCCTCGATGACAAGAGCAT
CAAGCCCGGCCTGGAAGCTTATAAACGCTAG

Upstream 100 bases:

>100_bases
TGCCGAGATTTTGATTAATGTGAATAACAGTTAATTGACATAACTATATTTGCGGTTTAGCTTTGAGTCATCTTCAAGCC
AAGAACAAGAGAGCATCGCC

Downstream 100 bases:

>100_bases
TCGGGTAACCAGAAGTTTTAAAGCCGAGTCTGTAGTTCCTGATAAAGATAATAAAGAGGAATCACCATGCTGGACGTGCC
CCTGCTGATCGGCGGCCAGT

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSKYEGRWSTIKVEIEQGIAWVILNRPEKRNAMSPTLNREMIDVLETLEQDPDAGVLVLTGAGEAWTAGMDLKEYFREVD
AGPEILQEKIRREASQWQWKMLRMYAKPTIAMVNGWCFGGGFSPLVACDLAICADEATFGLSEINWGIPPGNLVSKAMAD
TVGHRQSLYYIMTGKTFNGQKAAEMGLVNESVPLAQLRQVAIDLANNLLEKNPVVLRAAKHGFKRCRELTWEQNEDYLYA
KLDQSRLLDKEGGREQGMKQFLDDKSIKPGLEAYKR

Sequences:

>Translated_276_residues
MSKYEGRWSTIKVEIEQGIAWVILNRPEKRNAMSPTLNREMIDVLETLEQDPDAGVLVLTGAGEAWTAGMDLKEYFREVD
AGPEILQEKIRREASQWQWKMLRMYAKPTIAMVNGWCFGGGFSPLVACDLAICADEATFGLSEINWGIPPGNLVSKAMAD
TVGHRQSLYYIMTGKTFNGQKAAEMGLVNESVPLAQLRQVAIDLANNLLEKNPVVLRAAKHGFKRCRELTWEQNEDYLYA
KLDQSRLLDKEGGREQGMKQFLDDKSIKPGLEAYKR
>Mature_275_residues
SKYEGRWSTIKVEIEQGIAWVILNRPEKRNAMSPTLNREMIDVLETLEQDPDAGVLVLTGAGEAWTAGMDLKEYFREVDA
GPEILQEKIRREASQWQWKMLRMYAKPTIAMVNGWCFGGGFSPLVACDLAICADEATFGLSEINWGIPPGNLVSKAMADT
VGHRQSLYYIMTGKTFNGQKAAEMGLVNESVPLAQLRQVAIDLANNLLEKNPVVLRAAKHGFKRCRELTWEQNEDYLYAK
LDQSRLLDKEGGREQGMKQFLDDKSIKPGLEAYKR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=29.9595141700405, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI70995211, Length=241, Percent_Identity=29.045643153527, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI31542718, Length=205, Percent_Identity=28.2926829268293, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=229, Percent_Identity=27.5109170305677, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=229, Percent_Identity=27.5109170305677, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=229, Percent_Identity=27.5109170305677, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136756, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=29.277566539924, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788597, Length=204, Percent_Identity=33.8235294117647, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI221142681, Length=225, Percent_Identity=31.1111111111111, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=22.6765799256506, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=26.7326732673267, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=208, Percent_Identity=28.3653846153846, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=25.5813953488372, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=29.4573643410853, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=28.1746031746032, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=212, Percent_Identity=25.9433962264151, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=250, Percent_Identity=24, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=23.6607142857143, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=27.0142180094787, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=194, Percent_Identity=26.8041237113402, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=229, Percent_Identity=27.0742358078603, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129971, Length=274, Percent_Identity=25.9124087591241, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653477, Length=274, Percent_Identity=25.9124087591241, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21357171, Length=199, Percent_Identity=29.1457286432161, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=29.5918367346939, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653139, Length=209, Percent_Identity=31.1004784688995, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920382, Length=247, Percent_Identity=25.5060728744939, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=24.1509433962264, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583165, Length=283, Percent_Identity=22.2614840989399, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 31120; Mature: 30989

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYEGRWSTIKVEIEQGIAWVILNRPEKRNAMSPTLNREMIDVLETLEQDPDAGVLVLT
CCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
GAGEAWTAGMDLKEYFREVDAGPEILQEKIRREASQWQWKMLRMYAKPTIAMVNGWCFGG
CCCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECC
GFSPLVACDLAICADEATFGLSEINWGIPPGNLVSKAMADTVGHRQSLYYIMTGKTFNGQ
CCHHHHHHHHHHHCCCHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
KAAEMGLVNESVPLAQLRQVAIDLANNLLEKNPVVLRAAKHGFKRCRELTWEQNEDYLYA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCEEEE
KLDQSRLLDKEGGREQGMKQFLDDKSIKPGLEAYKR
ECCHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure 
SKYEGRWSTIKVEIEQGIAWVILNRPEKRNAMSPTLNREMIDVLETLEQDPDAGVLVLT
CCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
GAGEAWTAGMDLKEYFREVDAGPEILQEKIRREASQWQWKMLRMYAKPTIAMVNGWCFGG
CCCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECC
GFSPLVACDLAICADEATFGLSEINWGIPPGNLVSKAMADTVGHRQSLYYIMTGKTFNGQ
CCHHHHHHHHHHHCCCHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
KAAEMGLVNESVPLAQLRQVAIDLANNLLEKNPVVLRAAKHGFKRCRELTWEQNEDYLYA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCEEEE
KLDQSRLLDKEGGREQGMKQFLDDKSIKPGLEAYKR
ECCHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA