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The map label for this gene is aphE [H]

Identifier: 66045905

GI number: 66045905

Start: 3240608

End: 3241411

Strand: Direct

Name: aphE [H]

Synonym: Psyr_2669

Alternate gene names: 66045905

Gene position: 3240608-3241411 (Clockwise)

Preceding gene: 66045904

Following gene: 66045906

Centisome position: 53.18

GC content: 56.22

Gene sequence:

>804_bases
TTGAATCGAACTAATATTTTTTTTGGTGAATCGCATTCTGACTGGTTGCCTGTCAGAGGCGGAGAATCTGGTGATTTTGT
TTTTCGACGTGGTGACGGGCATGCCTTCGCGAAAATCGCACCTGCTTCCCGCCGCGGTGAGCTCGCTGGAGAGCGTGACC
GCCTCATTTGGCTCAAAGGTCGAGGTGTGGCTTGCCCCGAGGTGATCAACTGGCAGGAGGAACAGGAGGGTGCATGCTTG
GTGATAACGGCAATTCCGGGAGTACCGGCGGCTGATCTGTCTGGAGCGGATTTGCTCAAAGCGTGGCCGTCAATGGGGCA
GCAACTTGGCGCTGTTCACAGCCTATCGGTTGATCAATGTCCGTTTGAGCGCAGGCTGTCGCGAATGTTCGGACGCGCCG
TTGATGTGGTGTCCCGCAATGCCGTCAATCCCGACTTCTTACCGGACGAGGACAAGAGTACGCCGCAGCTCGATCTTTTG
GCTCGTGTCGAACGAGAGCTACCGGTGCGGCTCGACCAAGAGCGCACCGATATGGTTGTTTGCCATGGTGATCCCTGCAT
GCCGAACTTCATGGTGGACCCTAAAACTCTTCAATGCACGGGTCTGATCGACCTTGGGCGGCTCGGAACAGCAGATCGCT
ATGCCGATTTGGCACTCATGATTGCTAACGCCGAAGAGAACTGGGCAGCGCCAGATGAAGCAGAGCGCGCCTTCGCTGTC
CTATTCAATGTATTGGGGATCGAAGCCCCCGACCGCGAACGCCTTGCCTTCTATCTGCGATTGGACCCTCTGACTTGGGG
TTGA

Upstream 100 bases:

>100_bases
GAGACCATGGAAAGCATGTTCTCGGACTTACGTAGCAACTCGTTTCTTTTCGCAGGTTGAGCCACCTCCGCGCTTCATCA
GAAAACTGAAGGAACCTCCA

Downstream 100 bases:

>100_bases
TGTTCATGCCGCCTGTTTTTCCTGCTCATTGGCACGTTTCGCAACCTGTTCTCATTGCGGACACCTTTTCCAGCCTCGTT
TGGAAAGTTTCATTGCCAGA

Product: aminoglycoside phosphotransferase

Products: NA

Alternate protein names: Streptomycin 3''-phosphotransferase; Streptomycin 6-kinase; Streptomycin 6-phosphotransferase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLKGRGVACPEVINWQEEQEGACL
VITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERAFAV
LFNVLGIEAPDRERLAFYLRLDPLTWG

Sequences:

>Translated_267_residues
MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLKGRGVACPEVINWQEEQEGACL
VITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERAFAV
LFNVLGIEAPDRERLAFYLRLDPLTWG
>Mature_267_residues
MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLKGRGVACPEVINWQEEQEGACL
VITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERAFAV
LFNVLGIEAPDRERLAFYLRLDPLTWG

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.87 [H]

Molecular weight: Translated: 29587; Mature: 29587

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLKG
CCCCEEEECCCCCCEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEC
RGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSVDQC
CCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCC
PFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQERTDMVV
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCEEE
CHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERAFAV
ECCCCCCCCEEECCCEEEEECCEECCCCCCCHHHCEEEEEEECCCCCCCCCCHHHHHHHH
LFNVLGIEAPDRERLAFYLRLDPLTWG
HHHHHCCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLKG
CCCCEEEECCCCCCEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEC
RGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSVDQC
CCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCC
PFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQERTDMVV
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCEEE
CHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERAFAV
ECCCCCCCCEEECCCEEEEECCEECCCCCCCHHHCEEEEEEECCCCCCCCCCHHHHHHHH
LFNVLGIEAPDRERLAFYLRLDPLTWG
HHHHHCCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2167474; 2844130; 2821169 [H]