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The map label for this gene is nfrA1 [H]

Identifier: 66045891

GI number: 66045891

Start: 3225794

End: 3226615

Strand: Direct

Name: nfrA1 [H]

Synonym: Psyr_2655

Alternate gene names: 66045891

Gene position: 3225794-3226615 (Clockwise)

Preceding gene: 66045890

Following gene: 66045893

Centisome position: 52.94

GC content: 60.58

Gene sequence:

>822_bases
ATGACAGTCAACGCACAATCAAAACTGGCTTCCCGCTACGGCGCCGCGGACATCTCACCCCTCATGCCCTGGAACGAGAC
GATCGATCAGTTGCTCGACCATCGCAGCGTCCGTGCTTTCATTGATCAGCCACTGCCTGACGGCACCATCGAGACCCTGG
TCGCCGCGGCGCAGTCCGCCTCCACCTCTTCAAACCTGCAGTTCTGGAGCGTTGTCGCTGTTCAGGACAGCGATCGGAAA
GCGCGCCTCTCGGCACTGGCAGGAAACCAGGCCTACATCGGGCAGGCGCCTCTGTTCTTTGTATGGCTTGCCGACCTGTC
TAGGGTCACCCGCGTTGCTGAGCAGCAGGGTGTAGAGCTCGAAGCAGTGCCTTACTTGGAGAGCCTGTTGCTCGGTACGA
TTGACGCCGCCTTAGCCGCTCAGAACGCCGTGGTTGCTCTGGAGTCTCTGGGACTTGGGAGCGTGTACATCGGCGCCATC
CGAAACGACATTGAAGGCGTGGCCAAGGAGCTTGGTCTGCCTCCGCAGGTCTATCCAGTTTTCGGTCTCTGCGTAGGATA
TCCATCTACCGAGCGACCAGCTCAGGTAAAACCACGGCTCCCCCAAGGTGCAGTGCTTCACCGCGAGACCTACTCGGTAG
CGGCAGATGTGGAAGCGGTGGCTGTATACGATGAGCGCCTGGGCGCCTTCTACCAGCGTGAGGGCATGACGGCTTCCGGC
TGGTCGGAGCAAGTGGTCAATCGGCTTCGCAGTGTCTCGAGCCTGCATGGGCGAGAGGAGCTGGTTGAGGAGCTGAAGCG
GATGGGGTTTGGGCTGCGCTGA

Upstream 100 bases:

>100_bases
TGTGGGACTGCGTGGGCCCCATGGTATATGAAACTTCGCGCGGGAAGCGTGTAGCACTGCCGGCGATTTGGTAGCCTCGG
GCTCTATTTGGAGATCTCGA

Downstream 100 bases:

>100_bases
AGACTCATCGCGCCGCTGTCGGGCTGAAAATTGGCTCACCGTTACCTTGGCCCTGTTAAGCGAAGTTCACTAAAGGCACG
ACGCTTACATCGTCGCTCAC

Product: nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTVNAQSKLASRYGAADISPLMPWNETIDQLLDHRSVRAFIDQPLPDGTIETLVAAAQSASTSSNLQFWSVVAVQDSDRK
ARLSALAGNQAYIGQAPLFFVWLADLSRVTRVAEQQGVELEAVPYLESLLLGTIDAALAAQNAVVALESLGLGSVYIGAI
RNDIEGVAKELGLPPQVYPVFGLCVGYPSTERPAQVKPRLPQGAVLHRETYSVAADVEAVAVYDERLGAFYQREGMTASG
WSEQVVNRLRSVSSLHGREELVEELKRMGFGLR

Sequences:

>Translated_273_residues
MTVNAQSKLASRYGAADISPLMPWNETIDQLLDHRSVRAFIDQPLPDGTIETLVAAAQSASTSSNLQFWSVVAVQDSDRK
ARLSALAGNQAYIGQAPLFFVWLADLSRVTRVAEQQGVELEAVPYLESLLLGTIDAALAAQNAVVALESLGLGSVYIGAI
RNDIEGVAKELGLPPQVYPVFGLCVGYPSTERPAQVKPRLPQGAVLHRETYSVAADVEAVAVYDERLGAFYQREGMTASG
WSEQVVNRLRSVSSLHGREELVEELKRMGFGLR
>Mature_272_residues
TVNAQSKLASRYGAADISPLMPWNETIDQLLDHRSVRAFIDQPLPDGTIETLVAAAQSASTSSNLQFWSVVAVQDSDRKA
RLSALAGNQAYIGQAPLFFVWLADLSRVTRVAEQQGVELEAVPYLESLLLGTIDAALAAQNAVVALESLGLGSVYIGAIR
NDIEGVAKELGLPPQVYPVFGLCVGYPSTERPAQVKPRLPQGAVLHRETYSVAADVEAVAVYDERLGAFYQREGMTASGW
SEQVVNRLRSVSSLHGREELVEELKRMGFGLR

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=249, Percent_Identity=36.9477911646586, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 29599; Mature: 29468

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNAQSKLASRYGAADISPLMPWNETIDQLLDHRSVRAFIDQPLPDGTIETLVAAAQSA
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
STSSNLQFWSVVAVQDSDRKARLSALAGNQAYIGQAPLFFVWLADLSRVTRVAEQQGVEL
CCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
EAVPYLESLLLGTIDAALAAQNAVVALESLGLGSVYIGAIRNDIEGVAKELGLPPQVYPV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
FGLCVGYPSTERPAQVKPRLPQGAVLHRETYSVAADVEAVAVYDERLGAFYQREGMTASG
HHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
WSEQVVNRLRSVSSLHGREELVEELKRMGFGLR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVNAQSKLASRYGAADISPLMPWNETIDQLLDHRSVRAFIDQPLPDGTIETLVAAAQSA
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
STSSNLQFWSVVAVQDSDRKARLSALAGNQAYIGQAPLFFVWLADLSRVTRVAEQQGVEL
CCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
EAVPYLESLLLGTIDAALAAQNAVVALESLGLGSVYIGAIRNDIEGVAKELGLPPQVYPV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
FGLCVGYPSTERPAQVKPRLPQGAVLHRETYSVAADVEAVAVYDERLGAFYQREGMTASG
HHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
WSEQVVNRLRSVSSLHGREELVEELKRMGFGLR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]