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The map label for this gene is dhbF [H]

Identifier: 66045847

GI number: 66045847

Start: 3093701

End: 3095545

Strand: Reverse

Name: dhbF [H]

Synonym: Psyr_2611

Alternate gene names: 66045847

Gene position: 3095545-3093701 (Counterclockwise)

Preceding gene: 66045855

Following gene: 66045846

Centisome position: 50.8

GC content: 62.06

Gene sequence:

>1845_bases
ATGCCGATTACGAACACTGACGAATCGCTTAGCGCCGCGTCAGCCCCTCTCAAGCCCGGTGCTTTCCTGCACGAGATATT
CAGTGACAGGGCGCGTCAATTTCCTGAGCGCACCGCGGTGAGCGATGCAGCCCGGACGTTGAGCTACGCACAGCTCGACG
CGCTGTCGACAAAGCTGGCTGCACGTTTACGCGATGAAGGCGTGACCTACGGCACGCGTGTGGGCATGTACCTGCCGCGC
AGCGTCGATCTGGTCACCAGCCTGCTGGGTATTCTCAAAGCCGGCGGCACCTATGTGCCGGTCGACCCGCAATACCCTGG
CAAACGCGTCGAGCACATCGTGCGCGACAGCGAGCTGAGCCTGATCATCGGCGATGCCGCCAACCTGCCGAAAATATCGT
CTCTGCGCGTATTGGCACTCGACGAGTTACTGTCCGCGCCGGCGCTGCAACCGGCCGCTCAAGACACCCGTATCGACCCG
AATAATTCCACTGCCTACATCATCTACACCTCTGGCTCCACCGGTGAGCCCAAGGGCGTACAGGTTTCCCATGGCAACGT
CAGTCGCCTGCTGGAAAGTACCCAACGCGCCTATGGCTTCAACGCCCAGGATGTCTGGTCGATGTTCCACTCCATCGGTT
TCGACTTCTCGGTCTGGGAAATCTGGGGTGCCCTCGCCCACGGCGGCCAGGTGGCCGTTGTCCCGTATGACATCTCGCGC
TCTCCTGCCGCCCTGCGTCAATGGCTCGCCGATCAGCGCATCACCGTGCTCAGCCAGACGCCATCGGCTTTCCGTGGTCT
TGATGAGGCCGATCGTGGCAACACCGCGCCGCTGGCGCTGCGCTACGTGGTGCTCGGTGGCGAAGCGTTGCCCGCCAGCG
TGCTGCGTCCCTGGGTCGAGCGCCACGGTGACCAGAAGCCCGCGCTGATCAACATGTACGGCATCACTGAGGCCACCGTT
CACACCACCTTCAAGCGAGTGTTGGCGCAGGACCTGGAAACAGCAGCCATGGTGTCGCTGGGCAAGCCGCTGCACGGCTG
GCGCCTGCACCTGCTTGACGCCAACCAGGCGCCGGTCGCGGCAGGCACTACCGGCGAGCTGTACATCGAAGGCGCGGGCG
TGGCTCAGGGCTACCTGAACCGTGAGGCGCTCAACGTCGAGCGCTTCGTCGAGTTACCCGGTGCTGTGCGCGCCTATCGT
ACTGGCGACCTGATGACCCTGGAGAGTAATGGCGAATACCGTTATGCCGGTCGCTGCGACGAGCAATTGAAAATCAGCGG
TTTCCGCATCGAGCCCGGCGAGATCGAAGCCTCTCTGCAAACCAGCCCGAGCGTGGCCGCGGCCCATGTCGGCGTACATG
ATTACGGCGATGGTGACCTGCGCCTGGTAGCTTACGTGGTGCCAGGCCAGGGCGTCGACGCCTGGACCGAACAGGCTCGC
AGCGAAGTTGCCGCATTGATGGCCGAAAATTTGCCGGAGTACATGCGTCCCTCGGTGTATGTGCCGCTGGCAGAGCTGCC
GGTGACTCATCACGGCAAAATCGACAAGCAACAACTGCCCTCGCCTGCCGCTGGCACGGCGCTTTCAGGCGCGGCAGACG
TCAAGGGCCTCAGTGAACAGGAGCACTTCGTGCTCAAGGTCTGGAGCGAAGACCTGGGTCTGAAAAACATCGGCGTCAAT
GATGATTTCTTCGATTCCGGCGGCACTTCACTGGCACTGATCCGCTCGCTGAGCAAGCTCAAGACACATTACAAAATCAA
CCTCGACCCCGGGATCCTGGCGGATGGCGCAACGGCCAAAGTCCTGGCCGACCACATCACCCGTAGCCTTGTTCAAGCCC
ATTGA

Upstream 100 bases:

>100_bases
GGATCATGCCGGCCCATTGACGGATTCGTCAGGCCGCAGGGATGTCTCGATTGCAGCGGATTTGAGCGCTGCATCTGGTT
ATTACACAGGAGCTTTGGTC

Downstream 100 bases:

>100_bases
CCGAAAAGGAAAAGCTCATGAGCAAAAAATTCGCCTTAACTGCGGAACAGCGTGCCTCGTTCGAGAAAAACGGTTTTATC
GGGCCGTTTGACGCTTACTC

Product: amino acid adenylation

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MPITNTDESLSAASAPLKPGAFLHEIFSDRARQFPERTAVSDAARTLSYAQLDALSTKLAARLRDEGVTYGTRVGMYLPR
SVDLVTSLLGILKAGGTYVPVDPQYPGKRVEHIVRDSELSLIIGDAANLPKISSLRVLALDELLSAPALQPAAQDTRIDP
NNSTAYIIYTSGSTGEPKGVQVSHGNVSRLLESTQRAYGFNAQDVWSMFHSIGFDFSVWEIWGALAHGGQVAVVPYDISR
SPAALRQWLADQRITVLSQTPSAFRGLDEADRGNTAPLALRYVVLGGEALPASVLRPWVERHGDQKPALINMYGITEATV
HTTFKRVLAQDLETAAMVSLGKPLHGWRLHLLDANQAPVAAGTTGELYIEGAGVAQGYLNREALNVERFVELPGAVRAYR
TGDLMTLESNGEYRYAGRCDEQLKISGFRIEPGEIEASLQTSPSVAAAHVGVHDYGDGDLRLVAYVVPGQGVDAWTEQAR
SEVAALMAENLPEYMRPSVYVPLAELPVTHHGKIDKQQLPSPAAGTALSGAADVKGLSEQEHFVLKVWSEDLGLKNIGVN
DDFFDSGGTSLALIRSLSKLKTHYKINLDPGILADGATAKVLADHITRSLVQAH

Sequences:

>Translated_614_residues
MPITNTDESLSAASAPLKPGAFLHEIFSDRARQFPERTAVSDAARTLSYAQLDALSTKLAARLRDEGVTYGTRVGMYLPR
SVDLVTSLLGILKAGGTYVPVDPQYPGKRVEHIVRDSELSLIIGDAANLPKISSLRVLALDELLSAPALQPAAQDTRIDP
NNSTAYIIYTSGSTGEPKGVQVSHGNVSRLLESTQRAYGFNAQDVWSMFHSIGFDFSVWEIWGALAHGGQVAVVPYDISR
SPAALRQWLADQRITVLSQTPSAFRGLDEADRGNTAPLALRYVVLGGEALPASVLRPWVERHGDQKPALINMYGITEATV
HTTFKRVLAQDLETAAMVSLGKPLHGWRLHLLDANQAPVAAGTTGELYIEGAGVAQGYLNREALNVERFVELPGAVRAYR
TGDLMTLESNGEYRYAGRCDEQLKISGFRIEPGEIEASLQTSPSVAAAHVGVHDYGDGDLRLVAYVVPGQGVDAWTEQAR
SEVAALMAENLPEYMRPSVYVPLAELPVTHHGKIDKQQLPSPAAGTALSGAADVKGLSEQEHFVLKVWSEDLGLKNIGVN
DDFFDSGGTSLALIRSLSKLKTHYKINLDPGILADGATAKVLADHITRSLVQAH
>Mature_613_residues
PITNTDESLSAASAPLKPGAFLHEIFSDRARQFPERTAVSDAARTLSYAQLDALSTKLAARLRDEGVTYGTRVGMYLPRS
VDLVTSLLGILKAGGTYVPVDPQYPGKRVEHIVRDSELSLIIGDAANLPKISSLRVLALDELLSAPALQPAAQDTRIDPN
NSTAYIIYTSGSTGEPKGVQVSHGNVSRLLESTQRAYGFNAQDVWSMFHSIGFDFSVWEIWGALAHGGQVAVVPYDISRS
PAALRQWLADQRITVLSQTPSAFRGLDEADRGNTAPLALRYVVLGGEALPASVLRPWVERHGDQKPALINMYGITEATVH
TTFKRVLAQDLETAAMVSLGKPLHGWRLHLLDANQAPVAAGTTGELYIEGAGVAQGYLNREALNVERFVELPGAVRAYRT
GDLMTLESNGEYRYAGRCDEQLKISGFRIEPGEIEASLQTSPSVAAAHVGVHDYGDGDLRLVAYVVPGQGVDAWTEQARS
EVAALMAENLPEYMRPSVYVPLAELPVTHHGKIDKQQLPSPAAGTALSGAADVKGLSEQEHFVLKVWSEDLGLKNIGVND
DFFDSGGTSLALIRSLSKLKTHYKINLDPGILADGATAKVLADHITRSLVQAH

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=603, Percent_Identity=26.3681592039801, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI187761345, Length=545, Percent_Identity=26.4220183486239, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI187761343, Length=545, Percent_Identity=26.4220183486239, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI122937307, Length=505, Percent_Identity=24.7524752475248, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI38505220, Length=512, Percent_Identity=22.8515625, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI195947378, Length=414, Percent_Identity=23.4299516908213, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI58082049, Length=488, Percent_Identity=23.3606557377049, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786801, Length=601, Percent_Identity=33.4442595673877, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI145693145, Length=537, Percent_Identity=25.8845437616387, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=23.8095238095238, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786529, Length=536, Percent_Identity=24.8134328358209, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789201, Length=389, Percent_Identity=26.7352185089974, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=23.2, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17556356, Length=535, Percent_Identity=24.8598130841122, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=23.3663366336634, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=503, Percent_Identity=22.8628230616302, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32564422, Length=399, Percent_Identity=24.5614035087719, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32564420, Length=399, Percent_Identity=24.5614035087719, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=548, Percent_Identity=23.1751824817518, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=562, Percent_Identity=22.9537366548043, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=568, Percent_Identity=22.7112676056338, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=562, Percent_Identity=22.9537366548043, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=530, Percent_Identity=22.0754716981132, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=564, Percent_Identity=24.468085106383, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71983001, Length=564, Percent_Identity=24.468085106383, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=688, Percent_Identity=26.1627906976744, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6319264, Length=524, Percent_Identity=23.2824427480916, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=546, Percent_Identity=22.5274725274725, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648676, Length=535, Percent_Identity=30.8411214953271, Blast_Score=195, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24581924, Length=520, Percent_Identity=27.1153846153846, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24582852, Length=368, Percent_Identity=25.5434782608696, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859661, Length=551, Percent_Identity=25.0453720508167, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62472339, Length=557, Percent_Identity=21.903052064632, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24667955, Length=549, Percent_Identity=21.6757741347905, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355225, Length=533, Percent_Identity=22.5140712945591, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667959, Length=385, Percent_Identity=23.8961038961039, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 66239; Mature: 66107

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITNTDESLSAASAPLKPGAFLHEIFSDRARQFPERTAVSDAARTLSYAQLDALSTKLA
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARLRDEGVTYGTRVGMYLPRSVDLVTSLLGILKAGGTYVPVDPQYPGKRVEHIVRDSELS
HHHHHCCCCEEEEECEECCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCEE
LIIGDAANLPKISSLRVLALDELLSAPALQPAAQDTRIDPNNSTAYIIYTSGSTGEPKGV
EEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCE
QVSHGNVSRLLESTQRAYGFNAQDVWSMFHSIGFDFSVWEIWGALAHGGQVAVVPYDISR
EECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCC
SPAALRQWLADQRITVLSQTPSAFRGLDEADRGNTAPLALRYVVLGGEALPASVLRPWVE
CHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
RHGDQKPALINMYGITEATVHTTFKRVLAQDLETAAMVSLGKPLHGWRLHLLDANQAPVA
HCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
AGTTGELYIEGAGVAQGYLNREALNVERFVELPGAVRAYRTGDLMTLESNGEYRYAGRCD
CCCCCEEEEECCCHHHHHHCHHHHCHHHHHHCCCHHEEEECCCEEEEECCCCEEECCCCC
EQLKISGFRIEPGEIEASLQTSPSVAAAHVGVHDYGDGDLRLVAYVVPGQGVDAWTEQAR
CCEEEECEEECCCCEEEEECCCCCCEEEECCCEECCCCCEEEEEEEECCCCCHHHHHHHH
SEVAALMAENLPEYMRPSVYVPLAELPVTHHGKIDKQQLPSPAAGTALSGAADVKGLSEQ
HHHHHHHHHCCHHHHCCCEEEEHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCCCC
EHFVLKVWSEDLGLKNIGVNDDFFDSGGTSLALIRSLSKLKTHYKINLDPGILADGATAK
CCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCEECCCHHH
VLADHITRSLVQAH
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PITNTDESLSAASAPLKPGAFLHEIFSDRARQFPERTAVSDAARTLSYAQLDALSTKLA
CCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARLRDEGVTYGTRVGMYLPRSVDLVTSLLGILKAGGTYVPVDPQYPGKRVEHIVRDSELS
HHHHHCCCCEEEEECEECCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCEE
LIIGDAANLPKISSLRVLALDELLSAPALQPAAQDTRIDPNNSTAYIIYTSGSTGEPKGV
EEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCE
QVSHGNVSRLLESTQRAYGFNAQDVWSMFHSIGFDFSVWEIWGALAHGGQVAVVPYDISR
EECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCC
SPAALRQWLADQRITVLSQTPSAFRGLDEADRGNTAPLALRYVVLGGEALPASVLRPWVE
CHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
RHGDQKPALINMYGITEATVHTTFKRVLAQDLETAAMVSLGKPLHGWRLHLLDANQAPVA
HCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
AGTTGELYIEGAGVAQGYLNREALNVERFVELPGAVRAYRTGDLMTLESNGEYRYAGRCD
CCCCCEEEEECCCHHHHHHCHHHHCHHHHHHCCCHHEEEECCCEEEEECCCCEEECCCCC
EQLKISGFRIEPGEIEASLQTSPSVAAAHVGVHDYGDGDLRLVAYVVPGQGVDAWTEQAR
CCEEEECEEECCCCEEEEECCCCCCEEEECCCEECCCCCEEEEEEEECCCCCHHHHHHHH
SEVAALMAENLPEYMRPSVYVPLAELPVTHHGKIDKQQLPSPAAGTALSGAADVKGLSEQ
HHHHHHHHHCCHHHHCCCEEEEHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCCCC
EHFVLKVWSEDLGLKNIGVNDDFFDSGGTSLALIRSLSKLKTHYKINLDPGILADGATAK
CCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCEECCCHHH
VLADHITRSLVQAH
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]