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The map label for this gene is bphF [H]

Identifier: 66045824

GI number: 66045824

Start: 3034139

End: 3034915

Strand: Direct

Name: bphF [H]

Synonym: Psyr_2588

Alternate gene names: 66045824

Gene position: 3034139-3034915 (Clockwise)

Preceding gene: 66045823

Following gene: 66045825

Centisome position: 49.79

GC content: 63.58

Gene sequence:

>777_bases
ATGATGCTGAGGACCAATACGCTCAAAAGCAGGATTGCCGACGGACAGCCGGCACACGGGGTGATCAGTTCGATCCCGGC
CCCGGCCGCCATCGAGCTGATCGCCGAGGCCGGGTTCGACTTCGTGATCATCGACATGGAACACGTGCTGATCAACCCGG
AAACCGTGGAAAACATGATCCGCGTGGCAGAGAGCTACGACCTCACACCACTGGTGCGGGTCGCCGATCTCAACCCGAAA
ACCCTGCTGCGCCTGCTCGATGGCGGCGCGCAGGGCATCGTCTTGCCGATGATCGAGAGCCCCGAGCAACTGGCGGATGC
GATTGCCGCCTGCAAGTACCACCCGCTGGGTCGCCGCAGCCTCAATGCCGGCCGTCCCGGCTCGTTCGGCAAGCACAGCC
TGGCGCAGTACGTGGAGGCTGCCAATCAACAGATCATGGTGGTGGCGATGATCGAAAGCGCCGAAGGCGTGCGACGTGCC
GCCGAGATCGCCAGCGTGCCGGGTCTGGACATGATTCTTGAAGGCGCGGCAGACCTGTCACAGTCACTGGGCATGCCCTG
GCAGATCGATCAGCCAGATGTACAAAACGCGTTGCTTGCGACCTGGCAAGCCGCCCACGCGGCGGGCGTCGCCTATTGCG
CGATCCCCCGTCAACCCGGAGACGCTGCCCGCTGGCAGGCGCGCGGGGTCAACACCTTTGTCCTCGGGGATGAGCGCGGC
ATTGCCTTTCGCGCGCTTCAGGCCCGCCTGGCTCAACATTCAGCAGAAGGAAAATAA

Upstream 100 bases:

>100_bases
CATCGTACGAAGTGGAAGAACTGACCAAGCGCCGCCTGCTGGGTGATGGCGAGCGTCGCTTCCGCTCGGTACCTAACCCA
CTGCAAACATACAGGCCGCA

Downstream 100 bases:

>100_bases
CCCATGAACTTCACTTCACTCGCCGCTGACCTGGTCATGCAGGATCTGGTCGATTGCCTGCTGGCTGAAGATTTCTTTGG
CCGCGAGCCATTGCGCCTGC

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: HOA [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MMLRTNTLKSRIADGQPAHGVISSIPAPAAIELIAEAGFDFVIIDMEHVLINPETVENMIRVAESYDLTPLVRVADLNPK
TLLRLLDGGAQGIVLPMIESPEQLADAIAACKYHPLGRRSLNAGRPGSFGKHSLAQYVEAANQQIMVVAMIESAEGVRRA
AEIASVPGLDMILEGAADLSQSLGMPWQIDQPDVQNALLATWQAAHAAGVAYCAIPRQPGDAARWQARGVNTFVLGDERG
IAFRALQARLAQHSAEGK

Sequences:

>Translated_258_residues
MMLRTNTLKSRIADGQPAHGVISSIPAPAAIELIAEAGFDFVIIDMEHVLINPETVENMIRVAESYDLTPLVRVADLNPK
TLLRLLDGGAQGIVLPMIESPEQLADAIAACKYHPLGRRSLNAGRPGSFGKHSLAQYVEAANQQIMVVAMIESAEGVRRA
AEIASVPGLDMILEGAADLSQSLGMPWQIDQPDVQNALLATWQAAHAAGVAYCAIPRQPGDAARWQARGVNTFVLGDERG
IAFRALQARLAQHSAEGK
>Mature_258_residues
MMLRTNTLKSRIADGQPAHGVISSIPAPAAIELIAEAGFDFVIIDMEHVLINPETVENMIRVAESYDLTPLVRVADLNPK
TLLRLLDGGAQGIVLPMIESPEQLADAIAACKYHPLGRRSLNAGRPGSFGKHSLAQYVEAANQQIMVVAMIESAEGVRRA
AEIASVPGLDMILEGAADLSQSLGMPWQIDQPDVQNALLATWQAAHAAGVAYCAIPRQPGDAARWQARGVNTFVLGDERG
IAFRALQARLAQHSAEGK

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-oxovalerate to pyruvate and acetaldehyde (Probable) [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=30.3643724696356, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788578, Length=258, Percent_Identity=28.2945736434109, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 27599; Mature: 27599

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLRTNTLKSRIADGQPAHGVISSIPAPAAIELIAEAGFDFVIIDMEHVLINPETVENMI
CCCCCHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEHHHEEECHHHHHHHH
RVAESYDLTPLVRVADLNPKTLLRLLDGGAQGIVLPMIESPEQLADAIAACKYHPLGRRS
HHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCC
LNAGRPGSFGKHSLAQYVEAANQQIMVVAMIESAEGVRRAAEIASVPGLDMILEGAADLS
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHH
QSLGMPWQIDQPDVQNALLATWQAAHAAGVAYCAIPRQPGDAARWQARGVNTFVLGDERG
HHCCCCEECCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEEECCCC
IAFRALQARLAQHSAEGK
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMLRTNTLKSRIADGQPAHGVISSIPAPAAIELIAEAGFDFVIIDMEHVLINPETVENMI
CCCCCHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEHHHEEECHHHHHHHH
RVAESYDLTPLVRVADLNPKTLLRLLDGGAQGIVLPMIESPEQLADAIAACKYHPLGRRS
HHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCC
LNAGRPGSFGKHSLAQYVEAANQQIMVVAMIESAEGVRRAAEIASVPGLDMILEGAADLS
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHH
QSLGMPWQIDQPDVQNALLATWQAAHAAGVAYCAIPRQPGDAARWQARGVNTFVLGDERG
HHCCCCEECCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEEECCCC
IAFRALQARLAQHSAEGK
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9073078 [H]