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The map label for this gene is ycdT [H]

Identifier: 66045771

GI number: 66045771

Start: 2949567

End: 2950619

Strand: Direct

Name: ycdT [H]

Synonym: Psyr_2535

Alternate gene names: 66045771

Gene position: 2949567-2950619 (Clockwise)

Preceding gene: 66045769

Following gene: 66045772

Centisome position: 48.4

GC content: 57.74

Gene sequence:

>1053_bases
ATGAGCCTTCCGACGGAAGACCGCACCCAGCTGTTCAAGGACACGGCTATCCAGTTCTGGAAACACATCACCCCGATCGT
TTATGTCGCTCTGGGCATTCACTGTGTGTTGCTGACAGTGTTTCTGGGCCTGGGCATGAAGATCATGTGGATGGCGAACA
TCGTCAGCACGCTGATTTACGTCAACTGCATCTACCTGATCCGCAGGGGGCGGCATCGACAAGCCGGACAACTGATGTGC
CTGGAGATCATCGGCCATGCCGTGCTCGCGACATGGCAACTGGGCTGGGAAAGCAACTTCAGTTTTTACATATTTTGCGT
GATTCCGGTCATTGCCTTCACGTTTCAACTCAACGTCATCAGACGCATTGCCTACAGCGTGGCGATTCTGCTGTCGCTGG
TGGGCTGCTTCGTTTTTCGCCGATACACGGGGCAGGGAACCGGTCTGAGCCAGAACGTGCTCGATGCGTTCGGTATCGTC
AACGTACTCTCTGCGACCACTCTGTCGATTTACGCCACTGCACTGTCGGTACGTTTCACCTCGTCGATGCAACTGAGCCT
GTTTCATATCGCCAACCGCGACAGCCTGACCAATCTTTATACGCGCAGACGAGTGCTGCATCGCATACGCGAACTGGAAG
CCAGGCGGCAGGGGCTGTCGGCTTCGTTGATCCTGCTCGACATCGATCATTTCAAACAGATCAATGATATCCACGGGCAT
GAAACCGGCGATGTGGTTCTGCAACGGGTCGCCGAGATCATCGGTTCAAGCGTGCGCAGCAGCGATATGGCGGCGCGCTG
GGGCGGCGAGGAGTTTCTGGTGCTCATGCCTGACACCACGCCCGAAGACGCGCTGCGCGCGGCCGAACGTATCTGGCAGC
GTATCGCCGAAGAGGCCGGCCTGATCAACGAGCGCATCATTGCCGTCAGCGCTACAGTCGCGGTGGCGACCATCGCCACG
GGCGAGGCGTTCCAGCTGGCCCTCAATCGTGCCGACACGTCGCTGTATATGGGCAAACAGCAGGGCCGAAACCGGGTAAT
GATCGCCGGATGA

Upstream 100 bases:

>100_bases
GTCGCTGTATAAAGCGGCGTCGGTTCAGGCCGACAATACGTCATGATCTCAACCCTACGCCGAGGGTCATATTCCACCTG
CATTTCGAGATAACGTGATG

Downstream 100 bases:

>100_bases
GGTGCTTGCTCACGCCAGGCGCTACCAGCGTGTAGACTTTCGCGCTTTGAACCAGGGAGCACTGTGTCGTGCTTCCTCAT
CGGCGGTGTATCGATCAAGG

Product: GGDEF

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSLPTEDRTQLFKDTAIQFWKHITPIVYVALGIHCVLLTVFLGLGMKIMWMANIVSTLIYVNCIYLIRRGRHRQAGQLMC
LEIIGHAVLATWQLGWESNFSFYIFCVIPVIAFTFQLNVIRRIAYSVAILLSLVGCFVFRRYTGQGTGLSQNVLDAFGIV
NVLSATTLSIYATALSVRFTSSMQLSLFHIANRDSLTNLYTRRRVLHRIRELEARRQGLSASLILLDIDHFKQINDIHGH
ETGDVVLQRVAEIIGSSVRSSDMAARWGGEEFLVLMPDTTPEDALRAAERIWQRIAEEAGLINERIIAVSATVAVATIAT
GEAFQLALNRADTSLYMGKQQGRNRVMIAG

Sequences:

>Translated_350_residues
MSLPTEDRTQLFKDTAIQFWKHITPIVYVALGIHCVLLTVFLGLGMKIMWMANIVSTLIYVNCIYLIRRGRHRQAGQLMC
LEIIGHAVLATWQLGWESNFSFYIFCVIPVIAFTFQLNVIRRIAYSVAILLSLVGCFVFRRYTGQGTGLSQNVLDAFGIV
NVLSATTLSIYATALSVRFTSSMQLSLFHIANRDSLTNLYTRRRVLHRIRELEARRQGLSASLILLDIDHFKQINDIHGH
ETGDVVLQRVAEIIGSSVRSSDMAARWGGEEFLVLMPDTTPEDALRAAERIWQRIAEEAGLINERIIAVSATVAVATIAT
GEAFQLALNRADTSLYMGKQQGRNRVMIAG
>Mature_349_residues
SLPTEDRTQLFKDTAIQFWKHITPIVYVALGIHCVLLTVFLGLGMKIMWMANIVSTLIYVNCIYLIRRGRHRQAGQLMCL
EIIGHAVLATWQLGWESNFSFYIFCVIPVIAFTFQLNVIRRIAYSVAILLSLVGCFVFRRYTGQGTGLSQNVLDAFGIVN
VLSATTLSIYATALSVRFTSSMQLSLFHIANRDSLTNLYTRRRVLHRIRELEARRQGLSASLILLDIDHFKQINDIHGHE
TGDVVLQRVAEIIGSSVRSSDMAARWGGEEFLVLMPDTTPEDALRAAERIWQRIAEEAGLINERIIAVSATVAVATIATG
EAFQLALNRADTSLYMGKQQGRNRVMIAG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=38.5542168674699, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI87082007, Length=215, Percent_Identity=36.7441860465116, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693134, Length=217, Percent_Identity=33.6405529953917, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=39.5061728395062, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=35.672514619883, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787802, Length=245, Percent_Identity=30.6122448979592, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=241, Percent_Identity=29.045643153527, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=32.7160493827161, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081974, Length=172, Percent_Identity=33.1395348837209, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788381, Length=165, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=163, Percent_Identity=33.1288343558282, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39207; Mature: 39076

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPTEDRTQLFKDTAIQFWKHITPIVYVALGIHCVLLTVFLGLGMKIMWMANIVSTLIY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VNCIYLIRRGRHRQAGQLMCLEIIGHAVLATWQLGWESNFSFYIFCVIPVIAFTFQLNVI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHH
RRIAYSVAILLSLVGCFVFRRYTGQGTGLSQNVLDAFGIVNVLSATTLSIYATALSVRFT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
SSMQLSLFHIANRDSLTNLYTRRRVLHRIRELEARRQGLSASLILLDIDHFKQINDIHGH
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCC
ETGDVVLQRVAEIIGSSVRSSDMAARWGGEEFLVLMPDTTPEDALRAAERIWQRIAEEAG
CHHHHHHHHHHHHHCCHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
LINERIIAVSATVAVATIATGEAFQLALNRADTSLYMGKQQGRNRVMIAG
CHHHHHHEEEHHHHHHHHCCCHHHHHHHHHCCHHHHCCHHCCCCEEEEEC
>Mature Secondary Structure 
SLPTEDRTQLFKDTAIQFWKHITPIVYVALGIHCVLLTVFLGLGMKIMWMANIVSTLIY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VNCIYLIRRGRHRQAGQLMCLEIIGHAVLATWQLGWESNFSFYIFCVIPVIAFTFQLNVI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHH
RRIAYSVAILLSLVGCFVFRRYTGQGTGLSQNVLDAFGIVNVLSATTLSIYATALSVRFT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
SSMQLSLFHIANRDSLTNLYTRRRVLHRIRELEARRQGLSASLILLDIDHFKQINDIHGH
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCC
ETGDVVLQRVAEIIGSSVRSSDMAARWGGEEFLVLMPDTTPEDALRAAERIWQRIAEEAG
CHHHHHHHHHHHHHCCHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
LINERIIAVSATVAVATIATGEAFQLALNRADTSLYMGKQQGRNRVMIAG
CHHHHHHEEEHHHHHHHHCCCHHHHHHHHHCCHHHHCCHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]