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The map label for this gene is fliY [H]

Identifier: 66045739

GI number: 66045739

Start: 2913035

End: 2913886

Strand: Direct

Name: fliY [H]

Synonym: Psyr_2503

Alternate gene names: 66045739

Gene position: 2913035-2913886 (Clockwise)

Preceding gene: 66045738

Following gene: 66045740

Centisome position: 47.8

GC content: 57.39

Gene sequence:

>852_bases
ATGCATCGCGGACCTTCCTTTGTGAAAGCTTGTGCCTTTGTTCTTTCAGCCTCATTCATGCTGGCCAATACGGTGCAGGC
AGCCGAAGGCAGCAAGCTGGATGCAGTATTGAAACGTGGCAATCTGGTGGTAGGGACGGGCAGTACCAATGCTCCGTGGC
ACTTCCAGGGCGCAGACGGCAAGTTGCAGGGCTTCGATATCGACATTGGCAGAATCATCGCCAAGGGCCTGTTCAATGAT
CCGAGCAAGGTGGAGTTTGTCGTGCAGTCCTCGGATGCACGTATTCCCAACCTGCTGACCGACAAGGTCGACATCAGCTG
CCAGTTCATCACCGTTACTGCCAGTCGCGCGCAGCAGGTGGCATTCACCCTGCCGTACTACCGCGAGGGTGTGGCGCTGC
TGTTGCCTGCCAATAGCAAGTACAAGGAAATCGATGACCTGAAGGCGGCAGGTGACGATGTGACCGTGGCCGTACTGCAA
AACGTGTATGCCGAGGAACTGGTGCATCAGGCACTGCCCAAGGCCAAGGTCGATCAGTACGACAGCGTCGACCTGATGTA
CCAGGCGATCAACTCCGGGCGTGCCGATACAGCGGCCACCGATCAGTCTTCGGTCAAGTACCTGATGGTGCAGAACCCTG
GCCGCTACCGTTCGCCGGCCTTTGCCTGGAGCCCGCAAACCTATGCCTGCGCGGTCAAGCGTGGCGATCAGGACTGGCTG
AACTTCGTCAACACCGCGTTGCATGAGGCTATGACCGGTGTCGAGTTCCCGGCTTACGCGGCGTCTTTCAAGCAATGGTT
CGGTGTCGATCTGCCGGTGCCTGCAATCGGTTTTCCGATGGAATACAAATAA

Upstream 100 bases:

>100_bases
TGCGCATGTTCTCTGTGGCACACTTTCTGCTGTCTATTCCGTTGTTACATACCTGTATCCGCTATAGCGGAATAACGAAT
ACTTCGGCGGAGTTTAAGCC

Downstream 100 bases:

>100_bases
GTCCACTCTGACCTTCAGCGGCAGGGCGGGCCCTCGGGCTTTCCCTGCCAGGTACGGGCAAGCATGAATTACCAATTGAA
TTTTGATGCCGTATGGCGCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MHRGPSFVKACAFVLSASFMLANTVQAAEGSKLDAVLKRGNLVVGTGSTNAPWHFQGADGKLQGFDIDIGRIIAKGLFND
PSKVEFVVQSSDARIPNLLTDKVDISCQFITVTASRAQQVAFTLPYYREGVALLLPANSKYKEIDDLKAAGDDVTVAVLQ
NVYAEELVHQALPKAKVDQYDSVDLMYQAINSGRADTAATDQSSVKYLMVQNPGRYRSPAFAWSPQTYACAVKRGDQDWL
NFVNTALHEAMTGVEFPAYAASFKQWFGVDLPVPAIGFPMEYK

Sequences:

>Translated_283_residues
MHRGPSFVKACAFVLSASFMLANTVQAAEGSKLDAVLKRGNLVVGTGSTNAPWHFQGADGKLQGFDIDIGRIIAKGLFND
PSKVEFVVQSSDARIPNLLTDKVDISCQFITVTASRAQQVAFTLPYYREGVALLLPANSKYKEIDDLKAAGDDVTVAVLQ
NVYAEELVHQALPKAKVDQYDSVDLMYQAINSGRADTAATDQSSVKYLMVQNPGRYRSPAFAWSPQTYACAVKRGDQDWL
NFVNTALHEAMTGVEFPAYAASFKQWFGVDLPVPAIGFPMEYK
>Mature_283_residues
MHRGPSFVKACAFVLSASFMLANTVQAAEGSKLDAVLKRGNLVVGTGSTNAPWHFQGADGKLQGFDIDIGRIIAKGLFND
PSKVEFVVQSSDARIPNLLTDKVDISCQFITVTASRAQQVAFTLPYYREGVALLLPANSKYKEIDDLKAAGDDVTVAVLQ
NVYAEELVHQALPKAKVDQYDSVDLMYQAINSGRADTAATDQSSVKYLMVQNPGRYRSPAFAWSPQTYACAVKRGDQDWL
NFVNTALHEAMTGVEFPAYAASFKQWFGVDLPVPAIGFPMEYK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787031, Length=233, Percent_Identity=27.8969957081545, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788648, Length=174, Percent_Identity=28.1609195402299, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787085, Length=207, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788649, Length=173, Percent_Identity=29.4797687861272, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786876, Length=264, Percent_Identity=22.7272727272727, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787088, Length=227, Percent_Identity=24.2290748898678, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30917; Mature: 30917

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRGPSFVKACAFVLSASFMLANTVQAAEGSKLDAVLKRGNLVVGTGSTNAPWHFQGADG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCCCCCEEEECCCC
KLQGFDIDIGRIIAKGLFNDPSKVEFVVQSSDARIPNLLTDKVDISCQFITVTASRAQQV
CEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHCCCCCEEEEEEEEECCCCCEE
AFTLPYYREGVALLLPANSKYKEIDDLKAAGDDVTVAVLQNVYAEELVHQALPKAKVDQY
EEECCHHHCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
DSVDLMYQAINSGRADTAATDQSSVKYLMVQNPGRYRSPAFAWSPQTYACAVKRGDQDWL
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEEECCCHHHH
NFVNTALHEAMTGVEFPAYAASFKQWFGVDLPVPAIGFPMEYK
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHRGPSFVKACAFVLSASFMLANTVQAAEGSKLDAVLKRGNLVVGTGSTNAPWHFQGADG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCCCCCEEEECCCC
KLQGFDIDIGRIIAKGLFNDPSKVEFVVQSSDARIPNLLTDKVDISCQFITVTASRAQQV
CEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHCCCCCEEEEEEEEECCCCCEE
AFTLPYYREGVALLLPANSKYKEIDDLKAAGDDVTVAVLQNVYAEELVHQALPKAKVDQY
EEECCHHHCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
DSVDLMYQAINSGRADTAATDQSSVKYLMVQNPGRYRSPAFAWSPQTYACAVKRGDQDWL
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEEECCCHHHH
NFVNTALHEAMTGVEFPAYAASFKQWFGVDLPVPAIGFPMEYK
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]