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The map label for this gene is paaF [C]

Identifier: 66045704

GI number: 66045704

Start: 2860761

End: 2861636

Strand: Reverse

Name: paaF [C]

Synonym: Psyr_2468

Alternate gene names: 66045704

Gene position: 2861636-2860761 (Counterclockwise)

Preceding gene: 66045705

Following gene: 66045703

Centisome position: 46.96

GC content: 62.79

Gene sequence:

>876_bases
ATGACTTCGACCTCATTCGCAACCATAGAGCTGATCAACGACCCACGTGGCTTTGCCACGCTGTGGCTGAATCGATCGGA
CAAGAACAACGCCTTCAACGCGCAGATGATCCGCGAGCTGATTATCGCCCTGGACCAGGTACAAGGCGATGCCAGCCTGC
GCTTTCTGCTGTTGCGTGGCCGTGGCCGGCATTTCAGTGCTGGTGCCGACCTGGCGTGGATGCAGCAGGCAGCAGACCTG
GATTACAACACCAACCTGGACGACGCCCGCGAACTGGCGGAGCTGATGTACAGCCTCGCCAGGCTGAAAATCCCGACGCT
CGCCGTGGTCCAAGGCGCAGCTTTCGGCGGTGCGCTGGGCCTGATCAGTTGCTGTGACATGGCCATTGCAGCGGCGGATG
CGCAGTTCTGCCTGTCCGAAGTGCGCATCGGCCTGGCGCCGGCCGTTATCAGTCCATTCGTGGTACAGGCCATCGGCGAA
CGCGCTGCGCGACGTTACACCCTGACGGCCGAGCGTTTCGATGGCCAGCGGGCGCAGCAGATCGGTCTGGTCGCCGAGTG
CTACCCGGCTGCAGAACTGCAAGATCGAACGCTGCAATGGGTCGACAACCTGTTGCTCAACAGCCCGCAGGCCATGCGTG
TCAGCAAGGAGCTGCTGCGCGAAGTCGGCAGCGGTGTGCTGACCCCCGCGCTGCGTCGCTACTGCGAAAGCGCAATCGCG
CGTATCCGCACCAGTGCCGAAGGCCAGGAAGGCCTGCGCGCGTTTTTGCAGAAACGCACACCTGGCTGGCAGCCCGTCAA
GGATCAGCCGCCTGGCAATCCCGTTCCAGCAGCCTCGACAGCCCGGCACGAACCTCAGAAGGACTCGCAATCATGA

Upstream 100 bases:

>100_bases
AAACCCGCGACATTCTCGCGCTTGCCCTCTCCGCCTCGCTCAACGCGCCCATCGAACCTACCACCTTCGGCCTGTTCCGA
ATGTGAGCCGGGAAATGACC

Downstream 100 bases:

>100_bases
GCATGCCCGAGCTGACCACGCTGTTGATTGCCAACCGCGGCGAGATCGCCTGCCGCATCATGCGCACGGCGAAAAACATG
GGCCTTACCACGGTCGCCGT

Product: gamma-carboxygeranoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTSTSFATIELINDPRGFATLWLNRSDKNNAFNAQMIRELIIALDQVQGDASLRFLLLRGRGRHFSAGADLAWMQQAADL
DYNTNLDDARELAELMYSLARLKIPTLAVVQGAAFGGALGLISCCDMAIAAADAQFCLSEVRIGLAPAVISPFVVQAIGE
RAARRYTLTAERFDGQRAQQIGLVAECYPAAELQDRTLQWVDNLLLNSPQAMRVSKELLREVGSGVLTPALRRYCESAIA
RIRTSAEGQEGLRAFLQKRTPGWQPVKDQPPGNPVPAASTARHEPQKDSQS

Sequences:

>Translated_291_residues
MTSTSFATIELINDPRGFATLWLNRSDKNNAFNAQMIRELIIALDQVQGDASLRFLLLRGRGRHFSAGADLAWMQQAADL
DYNTNLDDARELAELMYSLARLKIPTLAVVQGAAFGGALGLISCCDMAIAAADAQFCLSEVRIGLAPAVISPFVVQAIGE
RAARRYTLTAERFDGQRAQQIGLVAECYPAAELQDRTLQWVDNLLLNSPQAMRVSKELLREVGSGVLTPALRRYCESAIA
RIRTSAEGQEGLRAFLQKRTPGWQPVKDQPPGNPVPAASTARHEPQKDSQS
>Mature_290_residues
TSTSFATIELINDPRGFATLWLNRSDKNNAFNAQMIRELIIALDQVQGDASLRFLLLRGRGRHFSAGADLAWMQQAADLD
YNTNLDDARELAELMYSLARLKIPTLAVVQGAAFGGALGLISCCDMAIAAADAQFCLSEVRIGLAPAVISPFVVQAIGER
AARRYTLTAERFDGQRAQQIGLVAECYPAAELQDRTLQWVDNLLLNSPQAMRVSKELLREVGSGVLTPALRRYCESAIAR
IRTSAEGQEGLRAFLQKRTPGWQPVKDQPPGNPVPAASTARHEPQKDSQS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=266, Percent_Identity=29.6992481203008, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542718, Length=259, Percent_Identity=25.0965250965251, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI70995211, Length=225, Percent_Identity=28, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=27.2030651340996, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260274832, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136756, Length=246, Percent_Identity=24.7967479674797, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136753, Length=246, Percent_Identity=24.7967479674797, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221307494, Length=246, Percent_Identity=24.7967479674797, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694516, Length=220, Percent_Identity=23.1818181818182, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI213417737, Length=220, Percent_Identity=23.1818181818182, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=31.0204081632653, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788597, Length=274, Percent_Identity=28.4671532846715, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=26.2172284644195, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=29.5275590551181, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=264, Percent_Identity=28.4090909090909, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=28.7937743190661, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=27.3148148148148, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=211, Percent_Identity=26.5402843601896, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=207, Percent_Identity=27.536231884058, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=21.8623481781377, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=25.7142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=27.3764258555133, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=25.2918287937743, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=25.2918287937743, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=28.3842794759825, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921018, Length=196, Percent_Identity=28.0612244897959, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922422, Length=269, Percent_Identity=22.3048327137546, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 31827; Mature: 31695

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTSFATIELINDPRGFATLWLNRSDKNNAFNAQMIRELIIALDQVQGDASLRFLLLRG
CCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
RGRHFSAGADLAWMQQAADLDYNTNLDDARELAELMYSLARLKIPTLAVVQGAAFGGALG
CCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHH
LISCCDMAIAAADAQFCLSEVRIGLAPAVISPFVVQAIGERAARRYTLTAERFDGQRAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCHHHHH
IGLVAECYPAAELQDRTLQWVDNLLLNSPQAMRVSKELLREVGSGVLTPALRRYCESAIA
HCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RIRTSAEGQEGLRAFLQKRTPGWQPVKDQPPGNPVPAASTARHEPQKDSQS
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSTSFATIELINDPRGFATLWLNRSDKNNAFNAQMIRELIIALDQVQGDASLRFLLLRG
CCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
RGRHFSAGADLAWMQQAADLDYNTNLDDARELAELMYSLARLKIPTLAVVQGAAFGGALG
CCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHH
LISCCDMAIAAADAQFCLSEVRIGLAPAVISPFVVQAIGERAARRYTLTAERFDGQRAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCHHHHH
IGLVAECYPAAELQDRTLQWVDNLLLNSPQAMRVSKELLREVGSGVLTPALRRYCESAIA
HCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RIRTSAEGQEGLRAFLQKRTPGWQPVKDQPPGNPVPAASTARHEPQKDSQS
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA