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The map label for this gene is aroE [C]

Identifier: 66045654

GI number: 66045654

Start: 2800692

End: 2801507

Strand: Direct

Name: aroE [C]

Synonym: Psyr_2418

Alternate gene names: 66045654

Gene position: 2800692-2801507 (Clockwise)

Preceding gene: 66045651

Following gene: 66045656

Centisome position: 45.96

GC content: 62.01

Gene sequence:

>816_bases
ATGTCCGTCACTGCTAACAGGGAGACATTATTGTGCATGTCCATGGCCGCCCGTCCCGGCAACTTTGGTGTGCGTTTCCA
TAATCATCTTTACCAGCAGTTAGGCTTGAACTTCTATTACAAGACCTTCGCCCCCAATGACCTTGCCGCCGCCGTGGCCG
GCATACGCTCGCTGGGGATTCGTGGCAGCGCAGTTTCGATGCCCTTCAAGGAGGCCTGCATCGAACTGGTCGACGAACTG
GACGCCTCGGCTGCCGCTATCCAGTCGATCAATACCATCGTCAACACCGACGGGCATCTGAAGGCGTTCAACACCGATTA
CATCGCCGTCGCTCAACTGATGGAGAGCCATGCGGTGCCGCGCAGCGCGTTTGCCCTGCGTGGAAGCGGGGGGATGGCCA
AGGCAGTGGCGTTCGCGCTGCGCGATGCGGGTTTCACCGAGGGGCTGATCGTGGCCCGCAACGAGCAGGCCGGCCGGCGT
CTGGCAGAGGCGTGCGGCTTTCGCTGGCAAGCGGAGCTGGGCAGTGCGCGCCCCCCCTTGCTGGTCAACGTCACTCCGTT
AGGCATGCAGGGCGCGCAAGCTGACGAACTGTCGTTTGACCTGCAAGCCATCGACGCTGCTGAAACGGTTTTCGATGTGG
TGGCTACCCCCGCCGACACGCCGCTGATCCAGGCTGCACGTCGTTTGGGCAAGCGTGTGGTGAGTGGTGATGAAGTGGCG
GCGATCCAGGCACTGGAGCAGTTTGTGCTCTATACCGGCGTGCGCCCGACCGACGAGCAGTATCAGCAGGCTGCAGCCTT
CGCAAGAGCCGGCTAG

Upstream 100 bases:

>100_bases
ATTTTATGTAAGGTGTGCCGCGTATGTCCTCGCGCTCACTGGTGCGTAGCATACGCTTCTATCGAAGATTCAAGCCATTA
CCGTCTGGAGGAAACACCCG

Downstream 100 bases:

>100_bases
CCGTCAGGTCGGGCAGGGCGCGTTACTCCTCGGCGTGCTCCAGTCTGGCCAGGCGTTCTTCAAGGGCCGCGACCCGTGCC
TCCAGTTCATCGATACGCGA

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSVTANRETLLCMSMAARPGNFGVRFHNHLYQQLGLNFYYKTFAPNDLAAAVAGIRSLGIRGSAVSMPFKEACIELVDEL
DASAAAIQSINTIVNTDGHLKAFNTDYIAVAQLMESHAVPRSAFALRGSGGMAKAVAFALRDAGFTEGLIVARNEQAGRR
LAEACGFRWQAELGSARPPLLVNVTPLGMQGAQADELSFDLQAIDAAETVFDVVATPADTPLIQAARRLGKRVVSGDEVA
AIQALEQFVLYTGVRPTDEQYQQAAAFARAG

Sequences:

>Translated_271_residues
MSVTANRETLLCMSMAARPGNFGVRFHNHLYQQLGLNFYYKTFAPNDLAAAVAGIRSLGIRGSAVSMPFKEACIELVDEL
DASAAAIQSINTIVNTDGHLKAFNTDYIAVAQLMESHAVPRSAFALRGSGGMAKAVAFALRDAGFTEGLIVARNEQAGRR
LAEACGFRWQAELGSARPPLLVNVTPLGMQGAQADELSFDLQAIDAAETVFDVVATPADTPLIQAARRLGKRVVSGDEVA
AIQALEQFVLYTGVRPTDEQYQQAAAFARAG
>Mature_270_residues
SVTANRETLLCMSMAARPGNFGVRFHNHLYQQLGLNFYYKTFAPNDLAAAVAGIRSLGIRGSAVSMPFKEACIELVDELD
ASAAAIQSINTIVNTDGHLKAFNTDYIAVAQLMESHAVPRSAFALRGSGGMAKAVAFALRDAGFTEGLIVARNEQAGRRL
AEACGFRWQAELGSARPPLLVNVTPLGMQGAQADELSFDLQAIDAAETVFDVVATPADTPLIQAARRLGKRVVSGDEVAA
IQALEQFVLYTGVRPTDEQYQQAAAFARAG

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=293, Percent_Identity=31.3993174061433, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789675, Length=244, Percent_Identity=28.2786885245902, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 28923; Mature: 28792

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTANRETLLCMSMAARPGNFGVRFHNHLYQQLGLNFYYKTFAPNDLAAAVAGIRSLGI
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCC
RGSAVSMPFKEACIELVDELDASAAAIQSINTIVNTDGHLKAFNTDYIAVAQLMESHAVP
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCC
RSAFALRGSGGMAKAVAFALRDAGFTEGLIVARNEQAGRRLAEACGFRWQAELGSARPPL
CHHEEEECCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCEEEHHCCCCCCCE
LVNVTPLGMQGAQADELSFDLQAIDAAETVFDVVATPADTPLIQAARRLGKRVVSGDEVA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
AIQALEQFVLYTGVRPTDEQYQQAAAFARAG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVTANRETLLCMSMAARPGNFGVRFHNHLYQQLGLNFYYKTFAPNDLAAAVAGIRSLGI
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCC
RGSAVSMPFKEACIELVDELDASAAAIQSINTIVNTDGHLKAFNTDYIAVAQLMESHAVP
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCC
RSAFALRGSGGMAKAVAFALRDAGFTEGLIVARNEQAGRRLAEACGFRWQAELGSARPPL
CHHEEEECCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCEEEHHCCCCCCCE
LVNVTPLGMQGAQADELSFDLQAIDAAETVFDVVATPADTPLIQAARRLGKRVVSGDEVA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
AIQALEQFVLYTGVRPTDEQYQQAAAFARAG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]