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The map label for this gene is tfdA [H]

Identifier: 66045590

GI number: 66045590

Start: 2729900

End: 2730739

Strand: Direct

Name: tfdA [H]

Synonym: Psyr_2354

Alternate gene names: 66045590

Gene position: 2729900-2730739 (Clockwise)

Preceding gene: 66045589

Following gene: 66045592

Centisome position: 44.8

GC content: 59.64

Gene sequence:

>840_bases
ATGTTAGCCACTTCGCTCGTTCAGCTGTCGCAGGAACCCATAAAGCCAGGTATTGGCTCGCGTATTTTCAACGGCAAGCA
GGAACTGCTGTCGGGTCGATTCGCTGGGGAGATCCGGGAACTGCTGGAGCAGCGAGGGGTGCTGGTGTTCCCGCAGATTC
ATTTTTCCGACGCAGAGCAGATTGCCTTTACTCGCACCCTGGGTACGTTCTGCCCTGAACCCGGCGATGGGCAGAACATC
ACCCGGATCAGCCTGGATGTGAGAGAGAATCCGGCAGGTGCGGAGTTTCTCAAAGGTTCGCTCTACTGGCACATCGACGG
TACGAGCAGCGATTCACCCATTCTGGCGTCGTTGTTGTCCTGCAAGGTGCGCGCCAGCTGGGGAGGCAACACGGGTTTCT
GTAACACTTACGCGGCGTACGAGGGGTTATCGAGCGCTGACCAACAGCATTACGACGGTCTGCGCGTCATTCATGCCCCA
TGGGCATCGTTACTCTATTACAATCCGGAGCCTGGCCTTGCAATGCTTGAGGCCATGCAGGTGATCGGCGAGAAGGAGTT
GCCGCTGGTCTGGAAGCACCGATCCGGTCGCAAGTCGCTGATTCTGGGCTGCACCGCGCAGCAGGTCGTTGGCCTCAGCC
TGGCGCAAAGTGCGCGGATTCTGGTCGGCCTTCGCGAGTGGGCCACGTCCGAGGCGTTCAGTTACAGCCATGCGTGGCAG
GTCGGTGATCTGGTGATCTGGGACAACACCGGCACCATGCACCGGGCCGAAGCCTATGACCCCGAGTGCGGGCGGATGAT
GCATCGTACCAAGTTGCAGGGCGAAGAGCCCTTCGCCTGA

Upstream 100 bases:

>100_bases
TACCTGCATTCCCTTTAAAACGTCCCGCGGCAAAACAGGGCTATTGCTTCCATTTCCTCATAAGTGTACCGTTGTGTTCA
CTTATGAAGAGGGTCGCACC

Downstream 100 bases:

>100_bases
TGCGCCGACTGCGAGAGCCGGTTGACGCCCAGGCTTTTATACTGAATGGCTCATTGTGAGCCGGATCGTTTTGCCGGATT
GGAGAAACGAGAATGTGCAG

Product: taurine catabolism dioxygenase TauD/TfdA

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLATSLVQLSQEPIKPGIGSRIFNGKQELLSGRFAGEIRELLEQRGVLVFPQIHFSDAEQIAFTRTLGTFCPEPGDGQNI
TRISLDVRENPAGAEFLKGSLYWHIDGTSSDSPILASLLSCKVRASWGGNTGFCNTYAAYEGLSSADQQHYDGLRVIHAP
WASLLYYNPEPGLAMLEAMQVIGEKELPLVWKHRSGRKSLILGCTAQQVVGLSLAQSARILVGLREWATSEAFSYSHAWQ
VGDLVIWDNTGTMHRAEAYDPECGRMMHRTKLQGEEPFA

Sequences:

>Translated_279_residues
MLATSLVQLSQEPIKPGIGSRIFNGKQELLSGRFAGEIRELLEQRGVLVFPQIHFSDAEQIAFTRTLGTFCPEPGDGQNI
TRISLDVRENPAGAEFLKGSLYWHIDGTSSDSPILASLLSCKVRASWGGNTGFCNTYAAYEGLSSADQQHYDGLRVIHAP
WASLLYYNPEPGLAMLEAMQVIGEKELPLVWKHRSGRKSLILGCTAQQVVGLSLAQSARILVGLREWATSEAFSYSHAWQ
VGDLVIWDNTGTMHRAEAYDPECGRMMHRTKLQGEEPFA
>Mature_279_residues
MLATSLVQLSQEPIKPGIGSRIFNGKQELLSGRFAGEIRELLEQRGVLVFPQIHFSDAEQIAFTRTLGTFCPEPGDGQNI
TRISLDVRENPAGAEFLKGSLYWHIDGTSSDSPILASLLSCKVRASWGGNTGFCNTYAAYEGLSSADQQHYDGLRVIHAP
WASLLYYNPEPGLAMLEAMQVIGEKELPLVWKHRSGRKSLILGCTAQQVVGLSLAQSARILVGLREWATSEAFSYSHAWQ
VGDLVIWDNTGTMHRAEAYDPECGRMMHRTKLQGEEPFA

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=286, Percent_Identity=29.3706293706294, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 30797; Mature: 30797

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATSLVQLSQEPIKPGIGSRIFNGKQELLSGRFAGEIRELLEQRGVLVFPQIHFSDAEQ
CCCHHHHHHHHCCCCCCCCCHHHCCHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCHHH
IAFTRTLGTFCPEPGDGQNITRISLDVRENPAGAEFLKGSLYWHIDGTSSDSPILASLLS
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHCCEEEEEECCCCCCCHHHHHHHH
CKVRASWGGNTGFCNTYAAYEGLSSADQQHYDGLRVIHAPWASLLYYNPEPGLAMLEAMQ
CEEEECCCCCCCCCCHHHHHCCCCCCCHHHCCCEEEEECCHHHEEEECCCCCHHHHHHHH
VIGEKELPLVWKHRSGRKSLILGCTAQQVVGLSLAQSARILVGLREWATSEAFSYSHAWQ
HHCCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHEEEHHHHHCHHHHCCCCCEE
VGDLVIWDNTGTMHRAEAYDPECGRMMHRTKLQGEEPFA
ECCEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLATSLVQLSQEPIKPGIGSRIFNGKQELLSGRFAGEIRELLEQRGVLVFPQIHFSDAEQ
CCCHHHHHHHHCCCCCCCCCHHHCCHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCHHH
IAFTRTLGTFCPEPGDGQNITRISLDVRENPAGAEFLKGSLYWHIDGTSSDSPILASLLS
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHCCEEEEEECCCCCCCHHHHHHHH
CKVRASWGGNTGFCNTYAAYEGLSSADQQHYDGLRVIHAPWASLLYYNPEPGLAMLEAMQ
CEEEECCCCCCCCCCHHHHHCCCCCCCHHHCCCEEEEECCHHHEEEECCCCCHHHHHHHH
VIGEKELPLVWKHRSGRKSLILGCTAQQVVGLSLAQSARILVGLREWATSEAFSYSHAWQ
HHCCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHEEEHHHHHCHHHHCCCCCEE
VGDLVIWDNTGTMHRAEAYDPECGRMMHRTKLQGEEPFA
ECCEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036764; 10777523; 11457355; 11955067 [H]