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The map label for this gene is braZ [H]

Identifier: 66045255

GI number: 66045255

Start: 2338610

End: 2339923

Strand: Direct

Name: braZ [H]

Synonym: Psyr_2015

Alternate gene names: 66045255

Gene position: 2338610-2339923 (Clockwise)

Preceding gene: 66045253

Following gene: 66045257

Centisome position: 38.38

GC content: 62.33

Gene sequence:

>1314_bases
ATGAAAGTGTTGAAAGGCCAGGACATCCTGGCACTCGGTTTTATGACATTTGCCCTGTTCGTCGGGGCTGGCAACATCAT
CTTTCCCCCCATAGTGGGTCTGCAGGCCGGGCCGCATGTATGGATGGCGGCGCTGGGCTTTCTGGTCACCGCGGTTGGCT
TGCCGGTCATTACCGTGATCGCTCTGGCCAGGGTCGGGGGCGCGATGGATGCCCTGAGCAGTCCGATTGGCAAGATCGCC
GGCGGGGCGCTCGCTGCCGTCTGCTACCTGGCGGTCGGCCCTTTGTTCGCCACTCCGCGTACCGCTACGGTCTCGTTCGA
AGTCGGTCTGGCGCCGCTGACCGGCGACACCCCGATGGCATTGTTCCTCTACAGTCTGGTGTATTTTCTGGTGGTGTTCT
GGGTTTCGCTGTACCCGGGGCGCTTGCTCGATACCGTCGGACGCTTTCTGGCGCCGCTCAAGATCATCGCGCTGGCGGTA
TTGGGCATCGCGGCATTCGCCTTGCCGGCAGGCGGAATAGGGGAGGCGGAGCCCGCGTACGCCGTAGCACCGTTTTCCCA
GGGCTTCATCAATGGTTACCTGACCATGGATACGCTGGGGGCGCTGGTCTTCGGTATCGTCATCGTCAACGCGATCCGCT
CTCGCGGTGTCGAATCGCCCCGTCTGATCACGCGTTACGCGATCATTGCCGGGCTGATTGCCGGAGTCGGGCTGGCGCTG
GTGTATGTCAGTCTGTTTCGCCTCGGTTCTGGCAGCCACGCCGTGGCGGCAGGTGCCAGCAACGGAGCGGCGGTTCTGCA
TGCCTACGTACAGCACACCTTCGGTTCGCTGGGCAGCGGATTCCTGGCCGTGCTGATTTCCCTGGCGTGTCTGGTCACTG
CAGTAGGCCTGACCTGTGCCTGCGCCGAATACTTTGCCAAGGTGCTGCCGCTGTCCTACAGAACGCTGGTGATCATCCTC
GCTGTGTTCTCGTTGCTGGTGTCCAATCTCGGGTTGACCAAACTGATCCAGTTTTCGATTCCGGTGCTCACGGCGATTTA
CCCGCCGTGCATCGTTCTGGTCGCGCTGAGCTTCTGCAAGGAGCTCTGGCAGAGCCAGGGACGCGTGGTGGCGCCTGTGA
TGCTGGTTTCGCTGGTGTTCGGCCTGATCGATGCGCTGAAGGGCGCCGGTTTCGGCGACGCACTGCCCGATGTTCTGAGC
CATCTGCCGCTGGGTGATCAGGGGCTTGCATGGCTGGTGCCCGCTGTTGTCACGCTGGCAGTTGCTGTGGTGGTAGACAG
GGTCATGGGCAAGCGCAGCGAGGCGCTGGCTTGA

Upstream 100 bases:

>100_bases
GTTTCGCAAGGTACTGCCACGTCTTACGCCGACGGGCTGCGTCTGCCCCATTCATGGGTGACGTCCCATCCCTTATTTCG
ATTCCGTGTGGTCCCTCGTA

Downstream 100 bases:

>100_bases
AGGCCGTGCTTTAGACGATTGCGCAGGAAAAAACGTGTTGCATGAAGAGGCCCGCTATAAGCGGGCCTCTTTAGTTCTGC
GAGCGTCTCAGCCTTACTTG

Product: branched chain amino acid ABC transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-III [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQAGPHVWMAALGFLVTAVGLPVITVIALARVGGAMDALSSPIGKIA
GGALAAVCYLAVGPLFATPRTATVSFEVGLAPLTGDTPMALFLYSLVYFLVVFWVSLYPGRLLDTVGRFLAPLKIIALAV
LGIAAFALPAGGIGEAEPAYAVAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPRLITRYAIIAGLIAGVGLAL
VYVSLFRLGSGSHAVAAGASNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCACAEYFAKVLPLSYRTLVIIL
AVFSLLVSNLGLTKLIQFSIPVLTAIYPPCIVLVALSFCKELWQSQGRVVAPVMLVSLVFGLIDALKGAGFGDALPDVLS
HLPLGDQGLAWLVPAVVTLAVAVVVDRVMGKRSEALA

Sequences:

>Translated_437_residues
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQAGPHVWMAALGFLVTAVGLPVITVIALARVGGAMDALSSPIGKIA
GGALAAVCYLAVGPLFATPRTATVSFEVGLAPLTGDTPMALFLYSLVYFLVVFWVSLYPGRLLDTVGRFLAPLKIIALAV
LGIAAFALPAGGIGEAEPAYAVAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPRLITRYAIIAGLIAGVGLAL
VYVSLFRLGSGSHAVAAGASNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCACAEYFAKVLPLSYRTLVIIL
AVFSLLVSNLGLTKLIQFSIPVLTAIYPPCIVLVALSFCKELWQSQGRVVAPVMLVSLVFGLIDALKGAGFGDALPDVLS
HLPLGDQGLAWLVPAVVTLAVAVVVDRVMGKRSEALA
>Mature_437_residues
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQAGPHVWMAALGFLVTAVGLPVITVIALARVGGAMDALSSPIGKIA
GGALAAVCYLAVGPLFATPRTATVSFEVGLAPLTGDTPMALFLYSLVYFLVVFWVSLYPGRLLDTVGRFLAPLKIIALAV
LGIAAFALPAGGIGEAEPAYAVAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPRLITRYAIIAGLIAGVGLAL
VYVSLFRLGSGSHAVAAGASNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCACAEYFAKVLPLSYRTLVIIL
AVFSLLVSNLGLTKLIQFSIPVLTAIYPPCIVLVALSFCKELWQSQGRVVAPVMLVSLVFGLIDALKGAGFGDALPDVLS
HLPLGDQGLAWLVPAVVTLAVAVVVDRVMGKRSEALA

Specific function: Component of the LIV-III transport system for branched- chain amino acids. BraZ is specific for isoleucine and valine. The LIV-III transport system may be H(+)-coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=429, Percent_Identity=68.0652680652681, Blast_Score=587, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45063; Mature: 45063

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQAGPHVWMAALGFLVTAVGLPVITVI
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALARVGGAMDALSSPIGKIAGGALAAVCYLAVGPLFATPRTATVSFEVGLAPLTGDTPMA
HHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
LFLYSLVYFLVVFWVSLYPGRLLDTVGRFLAPLKIIALAVLGIAAFALPAGGIGEAEPAY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
AVAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPRLITRYAIIAGLIAGVGLAL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VYVSLFRLGSGSHAVAAGASNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCA
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CAEYFAKVLPLSYRTLVIILAVFSLLVSNLGLTKLIQFSIPVLTAIYPPCIVLVALSFCK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELWQSQGRVVAPVMLVSLVFGLIDALKGAGFGDALPDVLSHLPLGDQGLAWLVPAVVTLA
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
VAVVVDRVMGKRSEALA
HHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQAGPHVWMAALGFLVTAVGLPVITVI
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALARVGGAMDALSSPIGKIAGGALAAVCYLAVGPLFATPRTATVSFEVGLAPLTGDTPMA
HHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
LFLYSLVYFLVVFWVSLYPGRLLDTVGRFLAPLKIIALAVLGIAAFALPAGGIGEAEPAY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
AVAPFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPRLITRYAIIAGLIAGVGLAL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VYVSLFRLGSGSHAVAAGASNGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCA
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CAEYFAKVLPLSYRTLVIILAVFSLLVSNLGLTKLIQFSIPVLTAIYPPCIVLVALSFCK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELWQSQGRVVAPVMLVSLVFGLIDALKGAGFGDALPDVLSHLPLGDQGLAWLVPAVVTLA
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
VAVVVDRVMGKRSEALA
HHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1900503; 10984043 [H]