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The map label for this gene is dgoD [H]

Identifier: 66045229

GI number: 66045229

Start: 2309056

End: 2310261

Strand: Direct

Name: dgoD [H]

Synonym: Psyr_1989

Alternate gene names: 66045229

Gene position: 2309056-2310261 (Clockwise)

Preceding gene: 66045228

Following gene: 66045230

Centisome position: 37.89

GC content: 61.11

Gene sequence:

>1206_bases
ATGCCGCCGTGCAGTAGCCGCTATCCAATAAGAGAATCAACAAGAGAAACCGCACCCATGAAAATCACCAAACTCACCAC
GTTCATCGTTCCGCCGCGCTGGTGCTTCCTCAAGGTCGAGACCGACGAGGGCGTTGTCGGCTGGGGCGAGCCAGTGGTCG
AAGGCCGCGCGCATACCGTCGCCGCTGCCGTGGAAGAGCTGTCCGATTACCTGATCGGCAAAGACCCGCGCAACATCGAA
GACATCTGGACGGTGCTGTATCGCGGCGGCTTCTACCGCGGCGGCGCGATCCACATGAGTGCGCTGGCCGGCATCGATCA
GGCGTTGTGGGACATCAAGGGCAAGGCGCTGGGGGTGTCGGTCAGCGACCTGCTCGGCGGTCAGGTGCGTGACAAGATTC
GCGTCTATTCGTGGATTGGCGGTGATCGTCCTGCCGACACGGCACGCGCCGCCAAGGAAGCGGTCGAGCGCGGTTTTACG
GCGGTGAAAATGAATGGCACCGAAGAGCTGCAGTTTCTCGATACCTTCGACAAGGTCGACCTGGCGCTGGCCAACGTGGC
CGCTGTGCGTGATGCGGTGGGGCCGAATGTCGGCATCGGTGTGGACTTCCACGGCCGGGTTCACAAGCCGATGGCCAAGG
TGCTGATGAAAGAGCTGGATCCGTACAAGCTGATGTTCATCGAAGAGCCGGTGCTCAGCGAGAACTATGAAGCACTGAAG
GAACTGGCGCCGCTGACCAGTACGCCGATTGCGCTGGGCGAGCGGTTGTTCTCGCGCTGGGACTTCAAGCGCGTATTGAG
CGAAGGCTACGTCGACATCATCCAGCCGGATGCCTCGCACGCCGGTGGCATTACCGAAACCCGCAAGATCGCCAACATGG
CTGAAGCCTACGACGTCGCACTGGCGTTGCACTGCCCGCTGGGGCCTATCGCGCTGGCGGCGTGCCTGCAACTGGACGCG
GTTTGCTACAACGCCTTCATCCAGGAGCAGAGCCTGGGTATCCACTACAACGAAAGCAACGACCTGCTCGATTACGTCAA
ACACCCGGAAGTCTTCGACTACGACAAAGGCATGGTCAAGATCCCCAACGGCCCCGGCCTGGGCATCGAAATCAACGAAG
AGTACGTCATCGAACGCGCCGCCATCGGCCACCGCTGGCGCAACCCGATCTGGCGTCATGCGGATGGCAGTTTTGCCGAG
TGGTAG

Upstream 100 bases:

>100_bases
ACCTGGCATGAGCGTCGCAGACGTCACCGAGCGTGCCCAGGCCTATGTGGCGGCCTGGAAGCAGCACAGCGCAGCGCTTG
CAGACTGACCGGCGCAGCAG

Downstream 100 bases:

>100_bases
AACAGCAAATCCGCTAGCCATCTTTTCGGCGCTCTGCGCTCATTGCCATACATAAGTCCTGAAGAGCCTGGAAAATGGGC
GTTCAGGCTTCTGCCCGCAG

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MPPCSSRYPIRESTRETAPMKITKLTTFIVPPRWCFLKVETDEGVVGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNIE
DIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVERGFT
AVKMNGTEELQFLDTFDKVDLALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALK
ELAPLTSTPIALGERLFSRWDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLDA
VCYNAFIQEQSLGIHYNESNDLLDYVKHPEVFDYDKGMVKIPNGPGLGIEINEEYVIERAAIGHRWRNPIWRHADGSFAE
W

Sequences:

>Translated_401_residues
MPPCSSRYPIRESTRETAPMKITKLTTFIVPPRWCFLKVETDEGVVGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNIE
DIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVERGFT
AVKMNGTEELQFLDTFDKVDLALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALK
ELAPLTSTPIALGERLFSRWDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLDA
VCYNAFIQEQSLGIHYNESNDLLDYVKHPEVFDYDKGMVKIPNGPGLGIEINEEYVIERAAIGHRWRNPIWRHADGSFAE
W
>Mature_400_residues
PPCSSRYPIRESTRETAPMKITKLTTFIVPPRWCFLKVETDEGVVGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNIED
IWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVERGFTA
VKMNGTEELQFLDTFDKVDLALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKE
LAPLTSTPIALGERLFSRWDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLDAV
CYNAFIQEQSLGIHYNESNDLLDYVKHPEVFDYDKGMVKIPNGPGLGIEINEEYVIERAAIGHRWRNPIWRHADGSFAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=62.82722513089, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1787864, Length=417, Percent_Identity=32.3741007194245, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI226510960, Length=297, Percent_Identity=30.6397306397306, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44453; Mature: 44322

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPCSSRYPIRESTRETAPMKITKLTTFIVPPRWCFLKVETDEGVVGWGEPVVEGRAHTV
CCCCCCCCCCHHCCCCCCCEEEEEEEEEEECCCEEEEEEECCCCCEECCCHHHCCCHHHH
AAAVEELSDYLIGKDPRNIEDIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVS
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECEE
VSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVERGFTAVKMNGTEELQFLDTFDKVD
HHHHHCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
LALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALK
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHH
ELAPLTSTPIALGERLFSRWDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVA
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHEE
LALHCPLGPIALAACLQLDAVCYNAFIQEQSLGIHYNESNDLLDYVKHPEVFDYDKGMVK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHCCCCCEECCCCEEE
IPNGPGLGIEINEEYVIERAAIGHRWRNPIWRHADGSFAEW
CCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PPCSSRYPIRESTRETAPMKITKLTTFIVPPRWCFLKVETDEGVVGWGEPVVEGRAHTV
CCCCCCCCCHHCCCCCCCEEEEEEEEEEECCCEEEEEEECCCCCEECCCHHHCCCHHHH
AAAVEELSDYLIGKDPRNIEDIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVS
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECEE
VSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVERGFTAVKMNGTEELQFLDTFDKVD
HHHHHCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
LALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALK
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHH
ELAPLTSTPIALGERLFSRWDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVA
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHEE
LALHCPLGPIALAACLQLDAVCYNAFIQEQSLGIHYNESNDLLDYVKHPEVFDYDKGMVK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHCCCCCEECCCCEEE
IPNGPGLGIEINEEYVIERAAIGHRWRNPIWRHADGSFAEW
CCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA