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The map label for this gene is yeiE [C]

Identifier: 66045222

GI number: 66045222

Start: 2301706

End: 2302578

Strand: Reverse

Name: yeiE [C]

Synonym: Psyr_1982

Alternate gene names: 66045222

Gene position: 2302578-2301706 (Counterclockwise)

Preceding gene: 66045232

Following gene: 66045221

Centisome position: 37.79

GC content: 61.17

Gene sequence:

>873_bases
ATGGACCTGGCCAACCTCAATGCCTTCATCGCCATTGCCGAGACCGGCAGTTTCTCCGGGGCAGGCGAACGCCTTTACCT
GACCCAACCGGCCATCAGCAAGCGCATCGCCGGTCTGGAGCAGCAACTCAACGTGCGCCTGTTCGATCGCCTGGGCCGCG
AAGTCAGTCTGACCGAGGCCGGACGCGCCCTGCTGCCACGCGCCTACCAGATTCTCAACGTGCTGGATGACACCCGCCGG
GCACTGACCAACCTGACCGGGGAAGTCACCGGGCGGCTGACTCTGGCGACCAGCCATCACATCGGCCTGCACCGTTTGCC
GCCGATATTACGGGCCTTTACCCGCACTTACCCGAAGGTTGCACTGGACATTCAGTTCCTGGATTCGGAAATCGCTTATG
AAGAAATTCTTCACGGGCGCGCCGAGTTGGCAGTCATTACACTGGCGCCCGAGCCGCATGCACTGATCCGCGCCGTGCCC
GTATGGGACGATCCGCTGGATTTCGTCGCTGCACCCGAGCACCCGCTGGCCAACAGCGGCCCGATGAAGCTGGAGGATAT
TGTGCGCTATCCGGCGGTATTTCCCGGCGGTAACACGTTTACCCATCACATTGTCAGCGGCTTGTGCGAGGCCCAGGGCC
TCAAGCCCAACATCGCCATGAGCACCAACTATATGGAGACCATCAAAATGATGGTTTCCATAGGCCTGGCCTGGAGCGTT
CTGCCGCGCACCATGCTCGACGAGCAGGTGGCGCGCATTCCGCTGCCGGGCGTACAGCTCAATCGCCAGCTAGGCTACAT
CCTGCACACCGAACGCACGCTATCGAATGCGGCACGGGCATTCATGAAACTGCTCGATGCGCAGACACAGTGA

Upstream 100 bases:

>100_bases
TGGCGATGACCCTTTGGCTTGTGCGGTCATCCTATGGAATATGCTTAAATAACTCAAATTCATAATTTTCATACTTTGGA
TAACCAACTGGAATCTGACC

Downstream 100 bases:

>100_bases
ATAGAGGCTGCAGGCCGCAGACAGATCGCTCTGGCGGCTCCATCGATCAAAGGCTCGGATACCATGCCCAACCCACCGGA
TAACGCCGCGCCGAAAACCA

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDLANLNAFIAIAETGSFSGAGERLYLTQPAISKRIAGLEQQLNVRLFDRLGREVSLTEAGRALLPRAYQILNVLDDTRR
ALTNLTGEVTGRLTLATSHHIGLHRLPPILRAFTRTYPKVALDIQFLDSEIAYEEILHGRAELAVITLAPEPHALIRAVP
VWDDPLDFVAAPEHPLANSGPMKLEDIVRYPAVFPGGNTFTHHIVSGLCEAQGLKPNIAMSTNYMETIKMMVSIGLAWSV
LPRTMLDEQVARIPLPGVQLNRQLGYILHTERTLSNAARAFMKLLDAQTQ

Sequences:

>Translated_290_residues
MDLANLNAFIAIAETGSFSGAGERLYLTQPAISKRIAGLEQQLNVRLFDRLGREVSLTEAGRALLPRAYQILNVLDDTRR
ALTNLTGEVTGRLTLATSHHIGLHRLPPILRAFTRTYPKVALDIQFLDSEIAYEEILHGRAELAVITLAPEPHALIRAVP
VWDDPLDFVAAPEHPLANSGPMKLEDIVRYPAVFPGGNTFTHHIVSGLCEAQGLKPNIAMSTNYMETIKMMVSIGLAWSV
LPRTMLDEQVARIPLPGVQLNRQLGYILHTERTLSNAARAFMKLLDAQTQ
>Mature_290_residues
MDLANLNAFIAIAETGSFSGAGERLYLTQPAISKRIAGLEQQLNVRLFDRLGREVSLTEAGRALLPRAYQILNVLDDTRR
ALTNLTGEVTGRLTLATSHHIGLHRLPPILRAFTRTYPKVALDIQFLDSEIAYEEILHGRAELAVITLAPEPHALIRAVP
VWDDPLDFVAAPEHPLANSGPMKLEDIVRYPAVFPGGNTFTHHIVSGLCEAQGLKPNIAMSTNYMETIKMMVSIGLAWSV
LPRTMLDEQVARIPLPGVQLNRQLGYILHTERTLSNAARAFMKLLDAQTQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=31.0580204778157, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=26.7361111111111, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI157672245, Length=225, Percent_Identity=31.5555555555556, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787879, Length=301, Percent_Identity=30.5647840531561, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787530, Length=255, Percent_Identity=28.6274509803922, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788748, Length=303, Percent_Identity=28.0528052805281, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788296, Length=218, Percent_Identity=31.1926605504587, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082132, Length=173, Percent_Identity=30.635838150289, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790399, Length=242, Percent_Identity=23.9669421487603, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786401, Length=305, Percent_Identity=23.6065573770492, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367136, Length=299, Percent_Identity=27.0903010033445, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=25.6849315068493, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788887, Length=251, Percent_Identity=27.0916334661355, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=27.2357723577236, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786713, Length=200, Percent_Identity=32.5, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787589, Length=193, Percent_Identity=28.4974093264249, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787601, Length=247, Percent_Identity=23.0769230769231, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081904, Length=288, Percent_Identity=22.5694444444444, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787128, Length=132, Percent_Identity=34.8484848484849, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=26.6932270916335, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788626, Length=184, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=27.2108843537415, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789440, Length=246, Percent_Identity=23.9837398373984, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082024, Length=190, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=128, Percent_Identity=33.59375, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786508, Length=177, Percent_Identity=28.2485875706215, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787949, Length=203, Percent_Identity=26.6009852216749, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788706, Length=124, Percent_Identity=35.4838709677419, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789506, Length=194, Percent_Identity=25.2577319587629, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI48994962, Length=80, Percent_Identity=42.5, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1 [H]

EC number: NA

Molecular weight: Translated: 32027; Mature: 32027

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLANLNAFIAIAETGSFSGAGERLYLTQPAISKRIAGLEQQLNVRLFDRLGREVSLTEA
CCCCCCEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
GRALLPRAYQILNVLDDTRRALTNLTGEVTGRLTLATSHHIGLHRLPPILRAFTRTYPKV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCHHHCCHHHHHHHHCCCCC
ALDIQFLDSEIAYEEILHGRAELAVITLAPEPHALIRAVPVWDDPLDFVAAPEHPLANSG
EEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEECCCCCCHHHCCCCCCCCCCCC
PMKLEDIVRYPAVFPGGNTFTHHIVSGLCEAQGLKPNIAMSTNYMETIKMMVSIGLAWSV
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHH
LPRTMLDEQVARIPLPGVQLNRQLGYILHTERTLSNAARAFMKLLDAQTQ
HHHHHHHHHHHHCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLANLNAFIAIAETGSFSGAGERLYLTQPAISKRIAGLEQQLNVRLFDRLGREVSLTEA
CCCCCCEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
GRALLPRAYQILNVLDDTRRALTNLTGEVTGRLTLATSHHIGLHRLPPILRAFTRTYPKV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCHHHCCHHHHHHHHCCCCC
ALDIQFLDSEIAYEEILHGRAELAVITLAPEPHALIRAVPVWDDPLDFVAAPEHPLANSG
EEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEECCCCCCHHHCCCCCCCCCCCC
PMKLEDIVRYPAVFPGGNTFTHHIVSGLCEAQGLKPNIAMSTNYMETIKMMVSIGLAWSV
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHH
LPRTMLDEQVARIPLPGVQLNRQLGYILHTERTLSNAARAFMKLLDAQTQ
HHHHHHHHHHHHCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9108283 [H]