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The map label for this gene is oprM [H]

Identifier: 66045208

GI number: 66045208

Start: 2281105

End: 2282493

Strand: Reverse

Name: oprM [H]

Synonym: Psyr_1968

Alternate gene names: 66045208

Gene position: 2282493-2281105 (Counterclockwise)

Preceding gene: 66045209

Following gene: 66045206

Centisome position: 37.46

GC content: 65.44

Gene sequence:

>1389_bases
ATGAAGCCGAGTCTCTCCGTTTTGACTGCCTGTCTGTTGCTGAGCGCCTGCCAGACACCCGCCCCGCCGCCGGACAGCGG
CATCCAGCCACCGGCAAGCTGGGCGTTTGCCGAAAACGCCGCCGCGCAGCGCAGCGACGTGCGTTGGTGGCAGCAGTTCG
GCAGCCCGCAGCTGGATCGCCTGATCGAACAGGCCAGCCGCGACAGCCACGAAGTCGCTGCGGCGATGGCGCGGGTTCGT
CAGGCACAGGCCAGCGCCATCATCGGCGGCGCGCCCTTGCTGCCGGAACTGGCCTTTGACCTGCGCGGCGAACGTAACCG
GTTCCTGCGCAACAGCGACCGCAAGGCCAACCCTTCCGACGACAACAACACCAACAATTTCACCAGCGACCTGACCGCCA
GCTATGAAGTGGATTTCTGGGGCGGCATCGCAGCGGGACGCGACAGCGCACTGCAAGGCCTGCGCGCCAGCCAGTTCGAT
CAGGCGACCGTGGAGCTGACCCTGCTGGCCAACGTCGCCGACCGCTATGCGCAAACCCTGGCGGCGCGCGAGCAAGGGCG
GATTGCCGAGCTGAATCTGGCCAACGCGCAAGACGTGCTGCGCCTGGTGCAGACCCGCTATGAATCGGGCTCGGCAACGG
CACTGGAGCTGGCACAGCAGAAGAATCTGGTTGCAACCCAGCAGCGTGAACTGCCCCGCGTTCAGCAACTGGCCAACGAG
CAGTTGATCACCCTGGCGGCCTTGCTCGGTCAGCCGGTGCAGAATCTGCACCTCGCGGATCAGCCCTTCGACACCCTGAA
CTGGCCGGACATCGGCGCAGGTGTACCGAGCGAGTTGCTGACCCGCCGCCCTGATCTGGCCAAGGCCGAGGCTGACCTGG
CAGCGGCACAGGCCAATGTCACCGTCGCCCGCGCCGCCATGCTGCCGAAACTGACCCTGAGCGCCAGGCTGGGCACCGAA
AACACCCGCGCCGAAGACTGGCTGCGTGCACCGTTCAGCAGCCTCGCTGCCGGGCTGGTGGGGCCGATCTTCAACAATGG
CCGCCTGGCTGCCGAGCGCGACAAGGCCACAGGGCGTCAGGAGGAACTGCTGGAAACCTATCGCGGCGCGATCATCAACA
GCTTCGCCGACGTGGAAAAAGCCCTGAACAGCATTGGCGGCCTGGATCAACAGCGCTACTGGCACGAAGAAGAACTCAAA
CAGGCACAAACTGCCTTCCGCATCGCCCAGAGCCGCTATCAGGAAGGCGCCGAGGACCTGCTCACCGTACTGGAAACCCA
GCGCACGCTGTACCTGGCGCAGGATGTCAGTGTGCAACTGCGGCTGGCGCGCCTGCAGGCGAGCATTGCGCTGTACAAGG
CACTGGGTGGGAGCTGGCAGGTGCACTGA

Upstream 100 bases:

>100_bases
GCCTGCGCCCTGATCACCGGCGTGATCTTCGGTTTCATGCCGGCCCGCAAGGCTGCCCGCCTCGACCCGGTCGCGGCCCT
CACCAGTGAATGATCGATCA

Downstream 100 bases:

>100_bases
GCGCCACGCAAGGAACGATAATCCCGACTACCGTGCTGAAGCACTGCGTCGGTCTGCGCGAACAACTCATTATCGTGCGA
CGCTCTGCGTCGCATGCCGT

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKPSLSVLTACLLLSACQTPAPPPDSGIQPPASWAFAENAAAQRSDVRWWQQFGSPQLDRLIEQASRDSHEVAAAMARVR
QAQASAIIGGAPLLPELAFDLRGERNRFLRNSDRKANPSDDNNTNNFTSDLTASYEVDFWGGIAAGRDSALQGLRASQFD
QATVELTLLANVADRYAQTLAAREQGRIAELNLANAQDVLRLVQTRYESGSATALELAQQKNLVATQQRELPRVQQLANE
QLITLAALLGQPVQNLHLADQPFDTLNWPDIGAGVPSELLTRRPDLAKAEADLAAAQANVTVARAAMLPKLTLSARLGTE
NTRAEDWLRAPFSSLAAGLVGPIFNNGRLAAERDKATGRQEELLETYRGAIINSFADVEKALNSIGGLDQQRYWHEEELK
QAQTAFRIAQSRYQEGAEDLLTVLETQRTLYLAQDVSVQLRLARLQASIALYKALGGSWQVH

Sequences:

>Translated_462_residues
MKPSLSVLTACLLLSACQTPAPPPDSGIQPPASWAFAENAAAQRSDVRWWQQFGSPQLDRLIEQASRDSHEVAAAMARVR
QAQASAIIGGAPLLPELAFDLRGERNRFLRNSDRKANPSDDNNTNNFTSDLTASYEVDFWGGIAAGRDSALQGLRASQFD
QATVELTLLANVADRYAQTLAAREQGRIAELNLANAQDVLRLVQTRYESGSATALELAQQKNLVATQQRELPRVQQLANE
QLITLAALLGQPVQNLHLADQPFDTLNWPDIGAGVPSELLTRRPDLAKAEADLAAAQANVTVARAAMLPKLTLSARLGTE
NTRAEDWLRAPFSSLAAGLVGPIFNNGRLAAERDKATGRQEELLETYRGAIINSFADVEKALNSIGGLDQQRYWHEEELK
QAQTAFRIAQSRYQEGAEDLLTVLETQRTLYLAQDVSVQLRLARLQASIALYKALGGSWQVH
>Mature_462_residues
MKPSLSVLTACLLLSACQTPAPPPDSGIQPPASWAFAENAAAQRSDVRWWQQFGSPQLDRLIEQASRDSHEVAAAMARVR
QAQASAIIGGAPLLPELAFDLRGERNRFLRNSDRKANPSDDNNTNNFTSDLTASYEVDFWGGIAAGRDSALQGLRASQFD
QATVELTLLANVADRYAQTLAAREQGRIAELNLANAQDVLRLVQTRYESGSATALELAQQKNLVATQQRELPRVQQLANE
QLITLAALLGQPVQNLHLADQPFDTLNWPDIGAGVPSELLTRRPDLAKAEADLAAAQANVTVARAAMLPKLTLSARLGTE
NTRAEDWLRAPFSSLAAGLVGPIFNNGRLAAERDKATGRQEELLETYRGAIINSFADVEKALNSIGGLDQQRYWHEEELK
QAQTAFRIAQSRYQEGAEDLLTVLETQRTLYLAQDVSVQLRLARLQASIALYKALGGSWQVH

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=459, Percent_Identity=28.5403050108932, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI2367348, Length=440, Percent_Identity=27.0454545454545, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50494; Mature: 50494

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSLSVLTACLLLSACQTPAPPPDSGIQPPASWAFAENAAAQRSDVRWWQQFGSPQLDR
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LIEQASRDSHEVAAAMARVRQAQASAIIGGAPLLPELAFDLRGERNRFLRNSDRKANPSD
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCC
DNNTNNFTSDLTASYEVDFWGGIAAGRDSALQGLRASQFDQATVELTLLANVADRYAQTL
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AAREQGRIAELNLANAQDVLRLVQTRYESGSATALELAQQKNLVATQQRELPRVQQLANE
HHHHCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
QLITLAALLGQPVQNLHLADQPFDTLNWPDIGAGVPSELLTRRPDLAKAEADLAAAQANV
HHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
TVARAAMLPKLTLSARLGTENTRAEDWLRAPFSSLAAGLVGPIFNNGRLAAERDKATGRQ
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHCCCHH
EELLETYRGAIINSFADVEKALNSIGGLDQQRYWHEEELKQAQTAFRIAQSRYQEGAEDL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTVLETQRTLYLAQDVSVQLRLARLQASIALYKALGGSWQVH
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPSLSVLTACLLLSACQTPAPPPDSGIQPPASWAFAENAAAQRSDVRWWQQFGSPQLDR
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LIEQASRDSHEVAAAMARVRQAQASAIIGGAPLLPELAFDLRGERNRFLRNSDRKANPSD
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCC
DNNTNNFTSDLTASYEVDFWGGIAAGRDSALQGLRASQFDQATVELTLLANVADRYAQTL
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AAREQGRIAELNLANAQDVLRLVQTRYESGSATALELAQQKNLVATQQRELPRVQQLANE
HHHHCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
QLITLAALLGQPVQNLHLADQPFDTLNWPDIGAGVPSELLTRRPDLAKAEADLAAAQANV
HHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
TVARAAMLPKLTLSARLGTENTRAEDWLRAPFSSLAAGLVGPIFNNGRLAAERDKATGRQ
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHCCCHH
EELLETYRGAIINSFADVEKALNSIGGLDQQRYWHEEELKQAQTAFRIAQSRYQEGAEDL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTVLETQRTLYLAQDVSVQLRLARLQASIALYKALGGSWQVH
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]