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The map label for this gene is lgrE [H]

Identifier: 66045184

GI number: 66045184

Start: 2216917

End: 2217675

Strand: Direct

Name: lgrE [H]

Synonym: Psyr_1944

Alternate gene names: 66045184

Gene position: 2216917-2217675 (Clockwise)

Preceding gene: 66045182

Following gene: 66045185

Centisome position: 36.38

GC content: 62.19

Gene sequence:

>759_bases
ATGAGCACGCCGGTGAGCCTGACGCTGTTCTGTCTGCCTTATTCCGGGGCCAGCGCGATGTTCTACAGCCCCTGGCGGCG
CAAATTGCCGGAGTGGCTGAACGTGCGCCCGCTGGAGCTTCCCGGCCGTGGCATGCGCATGGACGAGCCCTTGCAGACCG
ACATCGTGCAGCTGGCCAGTCATCTGGCCGACGAGATCAGTGCCGAACTGGACAAGCCCTATGCGCTGTTTGGCCACAGT
TTGGGCGGGCTGCTGGCCTTTGAGCTGGCGCACGTCTTGCGCGAGCGCGGTCTGCCTGCGCCGCTGGCACTGTTCGCATC
GGCGACTGCCGGCCCGGTGCGTCGCGATGTCAGCGAATACGCCACTGCGAAGACTGATGCGCAACTGCTCGACCGATTGC
GCACGTTAAAAGGCACCAGCGAAAACGTCATCGCCAACCAAGAGCTCATGCAGTTGATGCTACCGATCCTGCGCGCCGAT
TTCCTGCTCTGCGGCAGCTTTGTGTATGGCAAGCGCGAGCCGCTCAGCGTGCCGATCCATGTATTTGGCGGCAAGCAGGA
CAGCATCAGCGTCGAGCAACTGCTCGACTGGCAGGAAGAGACCTGCACCGGCTTCTCTCTAGACATGTTCGAGGGCCATC
ACTTTTATCTGGTCGACGAGCAAACGCAGCTGCTCCGTCTGCTGCGACGCTATTGCGAACAGCACCTGACCCGCTGGCGC
AACAGCGCATCACGGCAAATGAACCGGGCCGCCGGTTGA

Upstream 100 bases:

>100_bases
ACCACGGTTGCGGCCAGGGAATACCTCAGGAGAACCCCATGCGTTCCGATCACGCGCATCGCCGCTTTTACATCCGCGCC
CACAGCCGGACGCTTTCGAT

Downstream 100 bases:

>100_bases
CCCTGTTTGCCCCACGCGCGGCCTCCTTGCAGGGCGCGCGTCTTTTTTGAAATTCAGATTTTATCTGGCACGCCGAATCT
AGCAGGAACCCCATCATGGA

Product: thioesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSTPVSLTLFCLPYSGASAMFYSPWRRKLPEWLNVRPLELPGRGMRMDEPLQTDIVQLASHLADEISAELDKPYALFGHS
LGGLLAFELAHVLRERGLPAPLALFASATAGPVRRDVSEYATAKTDAQLLDRLRTLKGTSENVIANQELMQLMLPILRAD
FLLCGSFVYGKREPLSVPIHVFGGKQDSISVEQLLDWQEETCTGFSLDMFEGHHFYLVDEQTQLLRLLRRYCEQHLTRWR
NSASRQMNRAAG

Sequences:

>Translated_252_residues
MSTPVSLTLFCLPYSGASAMFYSPWRRKLPEWLNVRPLELPGRGMRMDEPLQTDIVQLASHLADEISAELDKPYALFGHS
LGGLLAFELAHVLRERGLPAPLALFASATAGPVRRDVSEYATAKTDAQLLDRLRTLKGTSENVIANQELMQLMLPILRAD
FLLCGSFVYGKREPLSVPIHVFGGKQDSISVEQLLDWQEETCTGFSLDMFEGHHFYLVDEQTQLLRLLRRYCEQHLTRWR
NSASRQMNRAAG
>Mature_251_residues
STPVSLTLFCLPYSGASAMFYSPWRRKLPEWLNVRPLELPGRGMRMDEPLQTDIVQLASHLADEISAELDKPYALFGHSL
GGLLAFELAHVLRERGLPAPLALFASATAGPVRRDVSEYATAKTDAQLLDRLRTLKGTSENVIANQELMQLMLPILRADF
LLCGSFVYGKREPLSVPIHVFGGKQDSISVEQLLDWQEETCTGFSLDMFEGHHFYLVDEQTQLLRLLRRYCEQHLTRWRN
SASRQMNRAAG

Specific function: In the final step of gramicidin biosynthesis, reduces the pentadecapeptide-aldehyde intermediate, that is released from the terminal module of the non-ribosomal peptide synthetase LgrD, to the final product ethanolamine-containing gramicidin [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=237, Percent_Identity=31.6455696202532, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI8922871, Length=205, Percent_Identity=33.1707317073171, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 28464; Mature: 28333

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPVSLTLFCLPYSGASAMFYSPWRRKLPEWLNVRPLELPGRGMRMDEPLQTDIVQLAS
CCCCCEEEEEEEECCCCCCCEECHHHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
HLADEISAELDKPYALFGHSLGGLLAFELAHVLRERGLPAPLALFASATAGPVRRDVSEY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHH
ATAKTDAQLLDRLRTLKGTSENVIANQELMQLMLPILRADFLLCGSFVYGKREPLSVPIH
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VFGGKQDSISVEQLLDWQEETCTGFSLDMFEGHHFYLVDEQTQLLRLLRRYCEQHLTRWR
EECCCCCCCCHHHHHCHHHHHCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
NSASRQMNRAAG
HHHHHHHHHCCC
>Mature Secondary Structure 
STPVSLTLFCLPYSGASAMFYSPWRRKLPEWLNVRPLELPGRGMRMDEPLQTDIVQLAS
CCCCEEEEEEEECCCCCCCEECHHHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
HLADEISAELDKPYALFGHSLGGLLAFELAHVLRERGLPAPLALFASATAGPVRRDVSEY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHH
ATAKTDAQLLDRLRTLKGTSENVIANQELMQLMLPILRADFLLCGSFVYGKREPLSVPIH
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VFGGKQDSISVEQLLDWQEETCTGFSLDMFEGHHFYLVDEQTQLLRLLRRYCEQHLTRWR
EECCCCCCCCHHHHHCHHHHHCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
NSASRQMNRAAG
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA