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The map label for this gene is prpB [C]

Identifier: 66045161

GI number: 66045161

Start: 2193153

End: 2193968

Strand: Reverse

Name: prpB [C]

Synonym: Psyr_1920

Alternate gene names: 66045161

Gene position: 2193968-2193153 (Counterclockwise)

Preceding gene: 66045170

Following gene: 66045160

Centisome position: 36.0

GC content: 56.37

Gene sequence:

>816_bases
ATGAACACCAGCCAGGCATTCAGAAAGTTACATGAACACGATGAGTTATTCGTCATGCCCAATGCTTGGTGTGAGGGAAG
CGCGCGCCTCATTCAGCAGTTGGGCTACGCCAGCCTCGGCACCACCAGCGCGGGGATTGCCTATGCACAGGGCTACCGCG
ATTCATCTCCCTCGATGGACAATGAAGATCGCTTTGCGGCGATTGAGCGCATCGTTCGGGCAGTGGACATACCCGTGACG
GCAGACCTGGAGAACGGTCTGGCGAAAACACTGGGCAAGGTCGAGGAGCACTTCAGAAGAGCCCTGACCGTCGGCTGCGC
CGGTGCGTCCATTGAGGATATATCCGACTATCATGACCCGGACACCCCGCTGTATTTCTCCATCGAAGAAGCGTGCGATC
GGGTGCGCGCTGCCAGTGAGGCCGCCCACGCGCTCGATGCCGATTTCATCGTCACCGCCAGGACCGACTATCTGCTTGGA
GCGCACGCGTATTCGTTGACCGAGGTTATTAAACGGCTGGTCGCGTTTGAAGAAGCTGGAGCCGATTGTCTGTTCGCACC
CGGCGTGAAGTCGATCGAGGACCTTGAGGCGATTCAGAGAGCGATCAGCAAACCTCTCAACGTCCTGCCTGTTTTCGGTA
TGACGATTGACAGACTCAGGACAGTCGGCGTTCGCCGGATCAGCCTGGGCAGTTCATTGTTTCGTAACGCCTACCGGCAG
ATCTCCACTGTTTTACTTGGATTGGAAGAACCAGAGGGGCTGGATTTTCTACAATCGGGACTGAGTCTGAATCAGCTCGA
TGAAGTGATGCGTTAG

Upstream 100 bases:

>100_bases
GGGCTGCTTGTTGTCTGTTCAAACTTTTTTAAGCCCCTTCACTTTTTTTTAATCATGCGTGCTGCGAAGAGGACTATGCT
TGGCCATTCAGGGAGGCGCC

Downstream 100 bases:

>100_bases
CCAGGATCGAGTGTTTGCATGGAGCATACATGTGATATCCGATAGAAACCGAAATCCCGTTATTGGTCTCGAGAACGGCG
TGACGATCAACCGTAGCTCC

Product: carboxyphosphonoenolpyruvate phosphonomutase-like protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNTSQAFRKLHEHDELFVMPNAWCEGSARLIQQLGYASLGTTSAGIAYAQGYRDSSPSMDNEDRFAAIERIVRAVDIPVT
ADLENGLAKTLGKVEEHFRRALTVGCAGASIEDISDYHDPDTPLYFSIEEACDRVRAASEAAHALDADFIVTARTDYLLG
AHAYSLTEVIKRLVAFEEAGADCLFAPGVKSIEDLEAIQRAISKPLNVLPVFGMTIDRLRTVGVRRISLGSSLFRNAYRQ
ISTVLLGLEEPEGLDFLQSGLSLNQLDEVMR

Sequences:

>Translated_271_residues
MNTSQAFRKLHEHDELFVMPNAWCEGSARLIQQLGYASLGTTSAGIAYAQGYRDSSPSMDNEDRFAAIERIVRAVDIPVT
ADLENGLAKTLGKVEEHFRRALTVGCAGASIEDISDYHDPDTPLYFSIEEACDRVRAASEAAHALDADFIVTARTDYLLG
AHAYSLTEVIKRLVAFEEAGADCLFAPGVKSIEDLEAIQRAISKPLNVLPVFGMTIDRLRTVGVRRISLGSSLFRNAYRQ
ISTVLLGLEEPEGLDFLQSGLSLNQLDEVMR
>Mature_271_residues
MNTSQAFRKLHEHDELFVMPNAWCEGSARLIQQLGYASLGTTSAGIAYAQGYRDSSPSMDNEDRFAAIERIVRAVDIPVT
ADLENGLAKTLGKVEEHFRRALTVGCAGASIEDISDYHDPDTPLYFSIEEACDRVRAASEAAHALDADFIVTARTDYLLG
AHAYSLTEVIKRLVAFEEAGADCLFAPGVKSIEDLEAIQRAISKPLNVLPVFGMTIDRLRTVGVRRISLGSSLFRNAYRQ
ISTVLLGLEEPEGLDFLQSGLSLNQLDEVMR

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=247, Percent_Identity=27.9352226720648, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 29757; Mature: 29757

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSQAFRKLHEHDELFVMPNAWCEGSARLIQQLGYASLGTTSAGIAYAQGYRDSSPSMD
CCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHCHHHHCCCCCCCCCCC
NEDRFAAIERIVRAVDIPVTADLENGLAKTLGKVEEHFRRALTVGCAGASIEDISDYHDP
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
DTPLYFSIEEACDRVRAASEAAHALDADFIVTARTDYLLGAHAYSLTEVIKRLVAFEEAG
CCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
ADCLFAPGVKSIEDLEAIQRAISKPLNVLPVFGMTIDRLRTVGVRRISLGSSLFRNAYRQ
CCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISTVLLGLEEPEGLDFLQSGLSLNQLDEVMR
HHHHHHCCCCCCCHHHHHHCCCHHHHHHHHC
>Mature Secondary Structure
MNTSQAFRKLHEHDELFVMPNAWCEGSARLIQQLGYASLGTTSAGIAYAQGYRDSSPSMD
CCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHCHHHHCCCCCCCCCCC
NEDRFAAIERIVRAVDIPVTADLENGLAKTLGKVEEHFRRALTVGCAGASIEDISDYHDP
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
DTPLYFSIEEACDRVRAASEAAHALDADFIVTARTDYLLGAHAYSLTEVIKRLVAFEEAG
CCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
ADCLFAPGVKSIEDLEAIQRAISKPLNVLPVFGMTIDRLRTVGVRRISLGSSLFRNAYRQ
CCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISTVLLGLEEPEGLDFLQSGLSLNQLDEVMR
HHHHHHCCCCCCCHHHHHHCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]