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The map label for this gene is pitA [H]

Identifier: 66045018

GI number: 66045018

Start: 2018928

End: 2020544

Strand: Reverse

Name: pitA [H]

Synonym: Psyr_1773

Alternate gene names: 66045018

Gene position: 2020544-2018928 (Counterclockwise)

Preceding gene: 66045019

Following gene: 66045015

Centisome position: 33.16

GC content: 61.47

Gene sequence:

>1617_bases
ATGGCTACAAACGCACTGTCTCCAGACGCTCTCGGCAAGGCGAGCCTGTCAGCGAGTCAATTCGGTCGCAAGCCCAGCCG
AATGACCATGATACTTTTCTTCACGATACTCATCGCAGGCTTGCTGTTCACCGGCTACAGCCTCAAACAGGACGTTGACG
ACATGGGCACGGTGGTCACCACCTGGACCCCGTTCGTGCTGTTGGGCGTAGCGTTGCTGATCGCCTTGGGCTTCGAGTTC
GTCAACGGCTTTCATGACACGGCCAACGCGGTCGCCACCGTGATCTACACCCACTCCCTGCCGCCCGGCTTTGCAGTGGT
CTGGTCAGGCTTCTTCAATTTTCTCGGCGTGATGTTTTCCAGCGGCGCGGTGGCATTCGGCATCATTGCCCTGCTGCCGG
TGGAACTGATCCTGCAAACCGGCTCATCGGCGGGCTTCGCGATGATCTTCGCGCTGTTGATCGCGGCGATCATCTGGAAC
CTCGGCACCTGGTGGCTGGGTCTGCCGGCGTCCTCGTCACACACGCTGATCGGTTCGATCATCGGCGTTGGCATCGCCAA
CGCCCTGATGCACGGGCGGGACGGCACCAGTGGCGTGGACTGGTCGCAGGCCACCAAGATCGGTTACTCACTGCTGCTCT
CGCCCCTGGTGGGCTTCGGCTGTGCGGCGTTGCTGTTGCTGGCCATGCGCATGTTCATCAAGAACCGCGCGCTGTACAAG
GCTCCGGAAGGCAATGCGCCGCCACCGTTGTGGATTCGCGCCATGCTGATCGTGACCTGCACGGGCGTGTCGTTCGCGCA
TGGCTCCAACGATGGGCAGAAAGGCATGGGCCTGATCATGCTGATTCTGGTCGGCACACTGCCGATGGCTTATGCGCTGA
ACCGCACCATGCCCGCCGAACAGGCTACCCAGTTCGCTGCCGTGGCTGAAGTAACCCAGCAGGCGCTGATCCGCAGCGTC
CCTCAACCGGCCCCCGGGGATGCGCGCAAGACGCTGTCGGATTACCTGGTCAGCAAGGAAGCGAGTCCTGAGCTGATCCC
TGCGCTGGCGGTCATGGCTGGCAAGATCGGCGATCAGGTCGCCAGTTATGGCTCGCTGTCTAAGGTGCCTGCCGAAGCCG
TGACCAACGTGCGTAACGACATGTATCTGACCTCGGAAAGCATCCGCCTGATGGAGAAAAACAAGGTCGGCGATTTCGAC
GCGGACACCAAGACCAAGCTTGAGGCGTTCAAGGGGCAGATCGACAGTGCTACGCGGTTCATTCCGACCTGGGTCAAGAT
TGTCGTGGCCATCGCCTTGGGTCTGGGCACGATGGTCGGCTGGAAGCGTATCGTGGTGACGGTGGGCGAGCGCATCGGCA
AGACGCACATGACTTACGCCCAGGGCGCGTCGGCAGAGGTCGTGGCGATGTTCACGATCGGCGCGGCGGACATGTATGGG
CTGCCGGTATCCACTACGCATGTGCTGTCGTCCGGCGTGGCCGGTTCGATGGTCGCCAATGGCTCGGGGCTGCAAATGCG
TACCCTGCGTAATCTGGCCACTGCCTGGGTGCTGACCCTGCCGGCGGCGATCCTCTTGTCAGGTTCGTTGTATTGGGTGT
TCAGCAAGCTGTTCTGA

Upstream 100 bases:

>100_bases
CAGAAAACCGGCGCAAAAGCGCAATCAATTGTTACATGTCTCACCGCTGTCACATTTTGCAATGACCATGTCAATATTCT
TTCAATCCAGGATTGCGAAC

Downstream 100 bases:

>100_bases
TGCCTGCTGCGCCCGCCCCCGCTTTGACTATCGTGCCGACGCTCTGCGTCGGCATGCAGTTCGTGACGCTCTGCGTTAGC
AATCTCTGATGCGAAACAAT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MATNALSPDALGKASLSASQFGRKPSRMTMILFFTILIAGLLFTGYSLKQDVDDMGTVVTTWTPFVLLGVALLIALGFEF
VNGFHDTANAVATVIYTHSLPPGFAVVWSGFFNFLGVMFSSGAVAFGIIALLPVELILQTGSSAGFAMIFALLIAAIIWN
LGTWWLGLPASSSHTLIGSIIGVGIANALMHGRDGTSGVDWSQATKIGYSLLLSPLVGFGCAALLLLAMRMFIKNRALYK
APEGNAPPPLWIRAMLIVTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPAEQATQFAAVAEVTQQALIRSV
PQPAPGDARKTLSDYLVSKEASPELIPALAVMAGKIGDQVASYGSLSKVPAEAVTNVRNDMYLTSESIRLMEKNKVGDFD
ADTKTKLEAFKGQIDSATRFIPTWVKIVVAIALGLGTMVGWKRIVVTVGERIGKTHMTYAQGASAEVVAMFTIGAADMYG
LPVSTTHVLSSGVAGSMVANGSGLQMRTLRNLATAWVLTLPAAILLSGSLYWVFSKLF

Sequences:

>Translated_538_residues
MATNALSPDALGKASLSASQFGRKPSRMTMILFFTILIAGLLFTGYSLKQDVDDMGTVVTTWTPFVLLGVALLIALGFEF
VNGFHDTANAVATVIYTHSLPPGFAVVWSGFFNFLGVMFSSGAVAFGIIALLPVELILQTGSSAGFAMIFALLIAAIIWN
LGTWWLGLPASSSHTLIGSIIGVGIANALMHGRDGTSGVDWSQATKIGYSLLLSPLVGFGCAALLLLAMRMFIKNRALYK
APEGNAPPPLWIRAMLIVTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPAEQATQFAAVAEVTQQALIRSV
PQPAPGDARKTLSDYLVSKEASPELIPALAVMAGKIGDQVASYGSLSKVPAEAVTNVRNDMYLTSESIRLMEKNKVGDFD
ADTKTKLEAFKGQIDSATRFIPTWVKIVVAIALGLGTMVGWKRIVVTVGERIGKTHMTYAQGASAEVVAMFTIGAADMYG
LPVSTTHVLSSGVAGSMVANGSGLQMRTLRNLATAWVLTLPAAILLSGSLYWVFSKLF
>Mature_537_residues
ATNALSPDALGKASLSASQFGRKPSRMTMILFFTILIAGLLFTGYSLKQDVDDMGTVVTTWTPFVLLGVALLIALGFEFV
NGFHDTANAVATVIYTHSLPPGFAVVWSGFFNFLGVMFSSGAVAFGIIALLPVELILQTGSSAGFAMIFALLIAAIIWNL
GTWWLGLPASSSHTLIGSIIGVGIANALMHGRDGTSGVDWSQATKIGYSLLLSPLVGFGCAALLLLAMRMFIKNRALYKA
PEGNAPPPLWIRAMLIVTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPAEQATQFAAVAEVTQQALIRSVP
QPAPGDARKTLSDYLVSKEASPELIPALAVMAGKIGDQVASYGSLSKVPAEAVTNVRNDMYLTSESIRLMEKNKVGDFDA
DTKTKLEAFKGQIDSATRFIPTWVKIVVAIALGLGTMVGWKRIVVTVGERIGKTHMTYAQGASAEVVAMFTIGAADMYGL
PVSTTHVLSSGVAGSMVANGSGLQMRTLRNLATAWVLTLPAAILLSGSLYWVFSKLF

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=477, Percent_Identity=42.9769392033543, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789360, Length=482, Percent_Identity=42.1161825726141, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 56932; Mature: 56800

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNALSPDALGKASLSASQFGRKPSRMTMILFFTILIAGLLFTGYSLKQDVDDMGTVVT
CCCCCCCCCCCCHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCHHH
TWTPFVLLGVALLIALGFEFVNGFHDTANAVATVIYTHSLPPGFAVVWSGFFNFLGVMFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
SGAVAFGIIALLPVELILQTGSSAGFAMIFALLIAAIIWNLGTWWLGLPASSSHTLIGSI
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHH
IGVGIANALMHGRDGTSGVDWSQATKIGYSLLLSPLVGFGCAALLLLAMRMFIKNRALYK
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
APEGNAPPPLWIRAMLIVTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPAE
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
QATQFAAVAEVTQQALIRSVPQPAPGDARKTLSDYLVSKEASPELIPALAVMAGKIGDQV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ASYGSLSKVPAEAVTNVRNDMYLTSESIRLMEKNKVGDFDADTKTKLEAFKGQIDSATRF
HHCCCCCCCCHHHHHHHHHCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHH
IPTWVKIVVAIALGLGTMVGWKRIVVTVGERIGKTHMTYAQGASAEVVAMFTIGAADMYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCHHHCC
LPVSTTHVLSSGVAGSMVANGSGLQMRTLRNLATAWVLTLPAAILLSGSLYWVFSKLF
CCCCHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
>Mature Secondary Structure 
ATNALSPDALGKASLSASQFGRKPSRMTMILFFTILIAGLLFTGYSLKQDVDDMGTVVT
CCCCCCCCCCCHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCHHH
TWTPFVLLGVALLIALGFEFVNGFHDTANAVATVIYTHSLPPGFAVVWSGFFNFLGVMFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
SGAVAFGIIALLPVELILQTGSSAGFAMIFALLIAAIIWNLGTWWLGLPASSSHTLIGSI
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHH
IGVGIANALMHGRDGTSGVDWSQATKIGYSLLLSPLVGFGCAALLLLAMRMFIKNRALYK
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
APEGNAPPPLWIRAMLIVTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPAE
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
QATQFAAVAEVTQQALIRSVPQPAPGDARKTLSDYLVSKEASPELIPALAVMAGKIGDQV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ASYGSLSKVPAEAVTNVRNDMYLTSESIRLMEKNKVGDFDADTKTKLEAFKGQIDSATRF
HHCCCCCCCCHHHHHHHHHCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHH
IPTWVKIVVAIALGLGTMVGWKRIVVTVGERIGKTHMTYAQGASAEVVAMFTIGAADMYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCHHHCC
LPVSTTHVLSSGVAGSMVANGSGLQMRTLRNLATAWVLTLPAAILLSGSLYWVFSKLF
CCCCHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]