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The map label for this gene is metZ [H]

Identifier: 66044914

GI number: 66044914

Start: 1873984

End: 1875195

Strand: Direct

Name: metZ [H]

Synonym: Psyr_1669

Alternate gene names: 66044914

Gene position: 1873984-1875195 (Clockwise)

Preceding gene: 66044913

Following gene: 66044915

Centisome position: 30.75

GC content: 62.29

Gene sequence:

>1212_bases
ATGACTCAGGAATGGGATGCAGGTCGGCTGGACAGCGACCTTGAAGGCGTAGGTTTCGATACCCTAGCCGTACGTGCCGG
GCATCACCGCACACCGGAAGGAGAGCATGGCGAGCCCATGTTCTTCACCTCCAGTTACGTATTTCGCACCGCCGCCGACG
CGGCTGCGCGTTTCTCCGGTGAGGTGCCGGGCAACGTCTACTCGCGTTACACCAACCCGACGGTGCGTTCGTTCGAAGAG
CGCATTGCCGCACTGGAAGGTGCCGAGCAGGCCGTCGCTACGGCGACCGGCATGGCGGCAACCCTGGCTGTGGTGATGAG
CCTGTGCAGCGCCGGTGATCATGTGCTGGTGTCACGCAGCGTCTTCGGCTCGACCATCAGCCTGTTCGACAAGTACTTCA
AGCGCTTTGGTGTCGAGGTGGATTACGTTCCTCTCGCCGATCTGAGCGGCTGGGACGCGGCGATCAAGCCCAACACCAAA
ATGCTCTTCGTCGAATCGCCGTCCAATCCCCTGGCTGAGCTGGTCGACATCGCGGCGCTGGCCGAGATCGCCCACGCCAA
GGGCGCGATGCTGATCGTCGACAACTGTTTCTGCACGCCGGCCTTGCAGCAGCCGCTGTTGCTGGGCGCTGATATTGTCG
TGCATTCGGCCACCAAGTTCATCGACGGCCAGGGCCGTTGCATGGGCGGCGTGGTTGCCGGGCGCAGCGAGCAGATGAAA
GAAGTGGTCGGCTTCCTGCGTACTGCCGGGCCGACGCTCAGCCCGTTCAACGCGTGGATCTTCCTTAAGGGCCTCGAAAC
CCTGAACCTGCGGATGCGCGCTCACTGTGCCAATGCTCAGGCACTGGCCGAGTGGCTCGAGCAGCAGGACGGCATCGAAA
AAGTCCATTACGCCGGACTGAAGAGTCACCCGCAGCACGAGTTGGCCCAGCGTCAGCAAAAGGGTTTCGGCGCTGTGGTG
AGCTTCGAGGTCAAGGGCGGCAAGGACGGCGCATGGCGCTTCATCGATGCCACACGACTGATTTCGATCACTGCCAACCT
GGGTGACAGCAAAACCACGATCACTCACCCGAGTACCACCTCTCACGGTCGCCTGGCACCTCAGGAGCGTGAAGCCGCAG
GTATTCGCGACAGCCTGATTCGGGTTGCAGTGGGGCTGGAAGACCTGGCTGACCTGCAGGCTGACCTGGCTCGTGGTCTG
GCGGCGTTGTGA

Upstream 100 bases:

>100_bases
GGATCGAGAGCGCGCGTAATGACGCCTCCAAGATCAAGACTCAGGCAGTGAGCGCGATCATCGATCTGTACAACAACTAA
TTGGGCAAGGAGTGGGCGGC

Downstream 100 bases:

>100_bases
GTAGCGAGGCCAGCAATCCGGCCAGTGGCAAGCCGGGCGAGGAGTGGGCGTTGAAATTTGCCAGTGTCCACAACGGTCGT
GTCGCTCTGGTGACCGGTGC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTQEWDAGRLDSDLEGVGFDTLAVRAGHHRTPEGEHGEPMFFTSSYVFRTAADAAARFSGEVPGNVYSRYTNPTVRSFEE
RIAALEGAEQAVATATGMAATLAVVMSLCSAGDHVLVSRSVFGSTISLFDKYFKRFGVEVDYVPLADLSGWDAAIKPNTK
MLFVESPSNPLAELVDIAALAEIAHAKGAMLIVDNCFCTPALQQPLLLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMRAHCANAQALAEWLEQQDGIEKVHYAGLKSHPQHELAQRQQKGFGAVV
SFEVKGGKDGAWRFIDATRLISITANLGDSKTTITHPSTTSHGRLAPQEREAAGIRDSLIRVAVGLEDLADLQADLARGL
AAL

Sequences:

>Translated_403_residues
MTQEWDAGRLDSDLEGVGFDTLAVRAGHHRTPEGEHGEPMFFTSSYVFRTAADAAARFSGEVPGNVYSRYTNPTVRSFEE
RIAALEGAEQAVATATGMAATLAVVMSLCSAGDHVLVSRSVFGSTISLFDKYFKRFGVEVDYVPLADLSGWDAAIKPNTK
MLFVESPSNPLAELVDIAALAEIAHAKGAMLIVDNCFCTPALQQPLLLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMRAHCANAQALAEWLEQQDGIEKVHYAGLKSHPQHELAQRQQKGFGAVV
SFEVKGGKDGAWRFIDATRLISITANLGDSKTTITHPSTTSHGRLAPQEREAAGIRDSLIRVAVGLEDLADLQADLARGL
AAL
>Mature_402_residues
TQEWDAGRLDSDLEGVGFDTLAVRAGHHRTPEGEHGEPMFFTSSYVFRTAADAAARFSGEVPGNVYSRYTNPTVRSFEER
IAALEGAEQAVATATGMAATLAVVMSLCSAGDHVLVSRSVFGSTISLFDKYFKRFGVEVDYVPLADLSGWDAAIKPNTKM
LFVESPSNPLAELVDIAALAEIAHAKGAMLIVDNCFCTPALQQPLLLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMKE
VVGFLRTAGPTLSPFNAWIFLKGLETLNLRMRAHCANAQALAEWLEQQDGIEKVHYAGLKSHPQHELAQRQQKGFGAVVS
FEVKGGKDGAWRFIDATRLISITANLGDSKTTITHPSTTSHGRLAPQEREAAGIRDSLIRVAVGLEDLADLQADLARGLA
AL

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=37.1951219512195, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI299473759, Length=332, Percent_Identity=33.433734939759, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=31.8042813455657, Blast_Score=175, Evalue=7e-44,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=37.2395833333333, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1789383, Length=358, Percent_Identity=27.6536312849162, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=336, Percent_Identity=40.1785714285714, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17560056, Length=325, Percent_Identity=40.6153846153846, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=34.4086021505376, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=396, Percent_Identity=35.3535353535354, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=33.8709677419355, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=26.5700483091787, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=328, Percent_Identity=27.7439024390244, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=346, Percent_Identity=28.3236994219653, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=36.9186046511628, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43313; Mature: 43181

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEWDAGRLDSDLEGVGFDTLAVRAGHHRTPEGEHGEPMFFTSSYVFRTAADAAARFSG
CCCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCC
EVPGNVYSRYTNPTVRSFEERIAALEGAEQAVATATGMAATLAVVMSLCSAGDHVLVSRS
CCCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
VFGSTISLFDKYFKRFGVEVDYVPLADLSGWDAAIKPNTKMLFVESPSNPLAELVDIAAL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEECCCCEEEEEECCCCHHHHHHHHHHH
AEIAHAKGAMLIVDNCFCTPALQQPLLLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
HHHHHCCCCEEEEECCCCCHHHCCCHHHCCHHHHHHHHHHCCCCCCEEECEECCCHHHHH
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMRAHCANAQALAEWLEQQDGIEKVHYAGL
HHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCC
KSHPQHELAQRQQKGFGAVVSFEVKGGKDGAWRFIDATRLISITANLGDSKTTITHPSTT
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCC
SHGRLAPQEREAAGIRDSLIRVAVGLEDLADLQADLARGLAAL
CCCCCCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQEWDAGRLDSDLEGVGFDTLAVRAGHHRTPEGEHGEPMFFTSSYVFRTAADAAARFSG
CCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCC
EVPGNVYSRYTNPTVRSFEERIAALEGAEQAVATATGMAATLAVVMSLCSAGDHVLVSRS
CCCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
VFGSTISLFDKYFKRFGVEVDYVPLADLSGWDAAIKPNTKMLFVESPSNPLAELVDIAAL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEECCCCEEEEEECCCCHHHHHHHHHHH
AEIAHAKGAMLIVDNCFCTPALQQPLLLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
HHHHHCCCCEEEEECCCCCHHHCCCHHHCCHHHHHHHHHHCCCCCCEEECEECCCHHHHH
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMRAHCANAQALAEWLEQQDGIEKVHYAGL
HHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCC
KSHPQHELAQRQQKGFGAVVSFEVKGGKDGAWRFIDATRLISITANLGDSKTTITHPSTT
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCC
SHGRLAPQEREAAGIRDSLIRVAVGLEDLADLQADLARGLAAL
CCCCCCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]